Introduction to PUMA Microscopy (the advanced 3D printed DIY microscope)

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  • เผยแพร่เมื่อ 7 พ.ย. 2024

ความคิดเห็น • 76

  • @PUMAMicroscope
    @PUMAMicroscope  11 หลายเดือนก่อน +2

    This project isn't *just* about building a DIY microscope but also education on optics and image processing. See my video on diffraction: th-cam.com/video/Ffm0A-H5ygE/w-d-xo.html or my series on Fourier analysis: th-cam.com/video/E8qWBaal6LM/w-d-xo.html and check out my playlists.

  • @TheKoodus
    @TheKoodus 2 ปีที่แล้ว +17

    Incredible. This is amongst the most shining examples of open source, both in spirit and in outcome. Thank you for your time and dedication to making a normally out of reach thing redily available!

    • @PUMAMicroscope
      @PUMAMicroscope  2 ปีที่แล้ว

      Thanks. Please tell others about the project - a lot more to come!

    • @TheKoodus
      @TheKoodus 2 ปีที่แล้ว

      @@PUMAMicroscope Amazing man I will. I don't have any real need for a microscope, but If i get myself going with a 3d printer I'll almost certainly make one of these....it's just amazing to see something of such quality at such a good price, and there are lots of kids where I live who would be fascinated I'm sure. If all of that happens, I'll be sure to let you know haha!!

  • @TheThundercow
    @TheThundercow 4 หลายเดือนก่อน +2

    Absolutely magnificent! This project inspires such wonderful good will and gratitude in me. These are the things that make the world just an objectively better place. I hope the PUMA system and open source projects like it gain the recognition and adoption it deserves. Thank you again for your hard work, generosity and candidness.

  • @aherbez
    @aherbez 3 ปีที่แล้ว +19

    Wow, this is amazing! Absolutely phenomenal work- thanks so much for putting this together and for releasing it as open-source!

    • @PUMAMicroscope
      @PUMAMicroscope  3 ปีที่แล้ว +5

      Thank you - I am hoping it will grow and develop as more people get to hear about it.

  • @oni2ink
    @oni2ink 3 ปีที่แล้ว +13

    That's a really impressive DIY microscope.
    It is truly mind blowing that you can make an epifluorescence microscope with this quality for 80 pounds.
    I'm not sure if I will try to build the microscope, but I will definitely follow the project!

    • @PUMAMicroscope
      @PUMAMicroscope  3 ปีที่แล้ว +4

      Thanks for the support. Soon I will be releasing the first full 'How to' video which shows exactly how to build the microscope stage from scratch - the single most complex component of all PUMA microscopes. Lot's more to come - stay tuned!

    • @evilkidm93b
      @evilkidm93b 3 ปีที่แล้ว +1

      I'm guessing that the majority of cost usually lies in the countless of hours spent designing it, not building it.

  • @ShopperPlug
    @ShopperPlug ปีที่แล้ว +3

    6:08 - wow, around £80 after 3D printing parts. This contribution is seriously immense, using advanced custom artificial intelligence software and hardware, can seriously automate microscopy at blazing speeds. I’m blown away with this project. Thank You.

    • @PUMAMicroscope
      @PUMAMicroscope  ปีที่แล้ว

      Thanks. Please let others know about the channel on your social media, etc. More videos are on the way - the next one will include an introduction to 3D printing for beginners.

  • @saurabhtripathi2598
    @saurabhtripathi2598 ปีที่แล้ว +2

    Just came across this DIY microscopy...great work👍

  • @mathiassmith8246
    @mathiassmith8246 3 ปีที่แล้ว +2

    This is totally cool. I know what I'm going to be doing once the snow flies this winter.

    • @PUMAMicroscope
      @PUMAMicroscope  3 ปีที่แล้ว

      Thanks for your support. Please spread the news about PUMA on your social media feeds. A new video on the Abbe condenser module will be released in the coming days so watch out for that. If you have any problems building or using a PUMA scope just post a comment and I will try to help - there are lots of practical tips, tricks and troubleshoot fixes that are just waiting to be shared.

  • @Stemfie3D
    @Stemfie3D 3 ปีที่แล้ว +9

    This is such a great project! Thank you for making this amazing microscope open-sourced!
    #PUMAmicroscope

  • @yevgeniy2005
    @yevgeniy2005 3 ปีที่แล้ว +3

    Wow, very impressive! Thank you very much for you hard work!!!

    • @PUMAMicroscope
      @PUMAMicroscope  3 ปีที่แล้ว +1

      Thank you. A new video will be uploaded soon - stay tuned!

  • @saurabhtripathi2598
    @saurabhtripathi2598 ปีที่แล้ว +1

    Can this be used for metallography ?

    • @PUMAMicroscope
      @PUMAMicroscope  ปีที่แล้ว

      Sure - see the video on 'epi-illumination' here: th-cam.com/video/cAEB10K8PqI/w-d-xo.html

  • @alexandrevaliquette3883
    @alexandrevaliquette3883 ปีที่แล้ว +3

    For the same total cost and same 1000x magnification, what combo will generally give a better image?
    25x Eyepiece + 40x Objective
    or
    10x Eyepiece + 100x Objective
    Thanks you for sharing your knowledge!!!!

    • @PUMAMicroscope
      @PUMAMicroscope  ปีที่แล้ว +4

      If, by 'better', you mean 'higher resolution' then the 100x objective scenario is better. This is because resolution is captured by the objective. The eyepiece just makes whatever the objective captures look bigger (and further away).

    • @alexandrevaliquette3883
      @alexandrevaliquette3883 ปีที่แล้ว +3

      @@PUMAMicroscope Yes, I mean "higher resolution". Your answer are like your video: you pack a lot of info in a small package! Thank you for the great explanation.

    • @alexandrevaliquette3883
      @alexandrevaliquette3883 ปีที่แล้ว +2

      @@PUMAMicroscopeSame question, but how about the field of view or other important parameter that might be modified between theses two configuration?

    • @PUMAMicroscope
      @PUMAMicroscope  ปีที่แล้ว

      Field of view will depend on the eyepiece field number (that is the field number of the x10 vs. the field number of the x20 eyepiece). @@alexandrevaliquette3883

    • @alexandrevaliquette3883
      @alexandrevaliquette3883 ปีที่แล้ว +1

      @@PUMAMicroscope So... Why don't we have one expensive 100x or 40x objective and many cheaper eyepiece with various magnification (0.1x 0.5x 1x 10x)?
      There must be a good reason we switch between objectives (and have a regular 10x eyepiece) instead of switching between eye piece (and having only one objective)?

  • @ThermalWorld_
    @ThermalWorld_ 11 หลายเดือนก่อน +1

    Wow 🤯
    Please support this project if you can..

  • @paaabl0.
    @paaabl0. 9 หลายเดือนก่อน +1

    Brilliant project!!! Thank you for sharing! Linux for microscopy

  • @WilliamRichardJames
    @WilliamRichardJames 9 หลายเดือนก่อน +1

    Very impressive ! I was intimidated at first , cause the accent makes me feel uneasy. Sort of like a gate crasher during best days at Bletchley Park facility. I'm impressed enough that maybe I will reengage my last two working brain cells and try to think of something useful to do with this instrument. Thx Much ! God Bless Bletchley Wizards ......... Nick , NavyBlueSmoke , LST -1195 , N.A.C. # 1274 , iss 20 Apr 72

    • @PUMAMicroscope
      @PUMAMicroscope  9 หลายเดือนก่อน

      Thanks. Stay tuned to the channel because in a later phase of the project I will be making applications videos. For now I am still teaching people how to build it (incl. some new modules) and Abbe's diffraction theory of microscope image formation.

  • @damiancavazos7043
    @damiancavazos7043 11 หลายเดือนก่อน +1

    Hello, could you please provide me with resources to understand schlieren phase contract. I can't find anything online

    • @PUMAMicroscope
      @PUMAMicroscope  11 หลายเดือนก่อน

      Hello. Essentially you are just unbalancing the frequency spectrum of the image. It is most commonly used in macro photography to image differences in air density (or other fluid). The Wikipedia article gives some background here: en.wikipedia.org/wiki/Schlieren_photography but I will try to explain more in some future videos.

    • @damiancavazos7043
      @damiancavazos7043 11 หลายเดือนก่อน +1

      @@PUMAMicroscope I will take a look, I have heard of schlieren photography but I don't know about the specifics. I suppose I am just wondering how it works in the PUMA scope. But I would love a video, I've learned a lot about microscopes through your work. Thanks!

    • @PUMAMicroscope
      @PUMAMicroscope  11 หลายเดือนก่อน

      I intend to make a video explaining more about Schlieren but that will be some time down the line, after I do my video series on photology (starting a couple of weeks from now) and some other stuff. Stay tuned.

  • @vijayautsarga
    @vijayautsarga 3 ปีที่แล้ว +3

    Amazing!

    • @PUMAMicroscope
      @PUMAMicroscope  3 ปีที่แล้ว

      Thanks. More videos are on the way. Stay tuned!

  • @sligit
    @sligit 2 ปีที่แล้ว +1

    Amazing work, congratulations.

  • @hjups
    @hjups 9 หลายเดือนก่อน +1

    Very cool!
    Have you considered a module to provide upper illumination for metallographic samples? I could see this being very useful for certain electronics hobbyists that have samples that would fit on the stage but are not translucent. Although that may require a different stage as well, since those microscopes usually have fine-grained X and Y adjustments.
    Having the ability to capture the objective output with a "real-time" camera would also be nice, since you could save images and view them on a monitor. If you couple that with a motorized X and Y adjust for the metallographic samples, then you could perform automated high-resolution sweeps that can be viewed on a computer afterwards.

    • @PUMAMicroscope
      @PUMAMicroscope  9 หลายเดือนก่อน +1

      Thanks. Those things are already part of the PUMA modular design. See these videos for details:
      Epi-Illumination (Dominus part 4): th-cam.com/video/cAEB10K8PqI/w-d-xo.html
      XY Vernier Slide Holder: th-cam.com/video/XtqqCiPloAQ/w-d-xo.html
      Stabiliser: th-cam.com/video/d8tMtWFhNY4/w-d-xo.html
      I also plan to make a new stage for the PUMA for more high end robotic control microscopy (for advanced university-type research) - but that is still in the pipeline. It will likely be more expensive to build than the current standard stage but is intended for people who need to go beyond manual visual observations. Other modules (e.g. for interferometry) are also under consideration - PUMA is still a developing project.

    • @communist754
      @communist754 9 หลายเดือนก่อน +1

      @@PUMAMicroscopeWow, if you do, I would have to get one for our lab! Do you have a page where I can donate to support this project?

    • @PUMAMicroscope
      @PUMAMicroscope  9 หลายเดือนก่อน

      Hello, sure. Each video description has donation links but here they are for convenience:
      1. Become a Patron: www.patreon.com/PUMAMicroscope
      2. Donate via PayPal: www.paypal.com/donate/?hosted_button_id=NPMYJKJATDLQ4
      Every donation helps - esp. when it comes to developing new hardware modules because there is a lot of 'try-fails' in the development cycle before I find the path that works well enough to become part of the PUMA canon. Thanks for any support you can provide, no matter how small.

  • @marchelandersen6839
    @marchelandersen6839 10 หลายเดือนก่อน +1

    this is amazing

  • @mamanekabirouousseini2824
    @mamanekabirouousseini2824 3 ปีที่แล้ว +1

    Amazing project

  • @thombaz
    @thombaz ปีที่แล้ว +1

    that tft filter is genius. such an obvious idea and no one used it

    • @PUMAMicroscope
      @PUMAMicroscope  ปีที่แล้ว

      Thanks. This video will show you how to build it: th-cam.com/video/yW9H66BlUjU/w-d-xo.html

  • @mikiex
    @mikiex 2 ปีที่แล้ว

    Amazing work

  • @sandorkonya
    @sandorkonya 3 ปีที่แล้ว +1

    Thank you Paul that you made this possible!
    The epi illumination is coaxial or at an angle?

    • @PUMAMicroscope
      @PUMAMicroscope  3 ปีที่แล้ว +1

      Thanks for your support. The epi-illumination is straight down (co-axial) via the 45 degree semi-silvered mirror in the advanced filter block. I got some great views of an integrated circuit with amazing resolution and clarity recently. I will try to find somewhere to share these pictures and make some more videos to show everyone how to use the various epi-illumination modes.

    • @sandorkonya
      @sandorkonya 3 ปีที่แล้ว +1

      @@PUMAMicroscope I saw the photo of a resistor in one of the videos however I was not sure whether it was coaxial, so thank you for the clarification!

    • @PUMAMicroscope
      @PUMAMicroscope  3 ปีที่แล้ว +1

      That picture was from a small CMOS camera module PCB - big PCBs won't fit on the stage. Interestingly I had to use epi-polarisation to get that clarity with the SMD components (to remove haze/glare) but the silicon chip IC dies I used give good images with plain epi-illumination (although epi-polarisation shows more as you rotate the polariser. I really need to make a video to illustrate that - it's on my list of videos to make).

  • @lotharmayring6063
    @lotharmayring6063 10 หลายเดือนก่อน +1

    why do you not use second hand professional microscops instead

    • @PUMAMicroscope
      @PUMAMicroscope  10 หลายเดือนก่อน +1

      Good question. Here is my answer.
      1. Second hand professional microscopes (SHPM) are more difficult to customise (other than minor adjustments) and most of them are much less portable (esp. when you consider the range of imaging modalities available on them. See, for example, my video on epi-illumination th-cam.com/video/cAEB10K8PqI/w-d-xo.html ).
      2. SHPM are also variable (you can make 20 PUMAs all the same for a research project or class - not so easy or cheap to do that with SHPM).
      3. Of course you can use SHPM *as well*. PUMA and SHPM are not mutually exclusive.
      4. Getting the same range of imaging modality features from a SHPM may be much more expensive (like fluoresence or epi-illumination) or simply not available (like augmented reality attachments and combinations of modalities that PUMA can do) and if they are available it is not so quick and easy (or even possible in some cases) to switch between them as with the modules of a PUMA.
      5. Also PUMA is about microscopy education - learning how various aspects of advanced microscopy work by building a scope from scratch is good practical education. My videos also emphasise theory as well as practice.
      These points are a recurring theme in the other videos in this series.

    • @lotharmayring6063
      @lotharmayring6063 10 หลายเดือนก่อน

      @@PUMAMicroscope you can never reach the mechanic qualiy of an Zeiss axiscope etc . I bought one from al university lab for a few bugs

    • @lotharmayring6063
      @lotharmayring6063 10 หลายเดือนก่อน

      @@PUMAMicroscope Viele Mikroskopiker meinen, dass die Technik des Mikroskopes und dessen Optik das Wichtigste
      am Mikroskopieren ist. Das ist ein grosser Irrtum, denn mit einen einfachen gebrauchten
      Labormikroskop der 70iger Jahre lassen sich zumindest im Hobbybereich durchaus
      brauchbare Ergebnisse erzielen. Heute versuchen sich die sog Mikrofreaks in der Perfektion ihrer
      Abbildung zu uebertreffen und veranstalten dafuer sogar Wettbewerbe. Das Wichtigste am Mikroskopieren
      ist jedoch die Arbeit draussen in der Natur und die Paeparationstechnik. Dafuer sind auch fundierte Kenntnisse
      in Biologie und Chemie sowie eine umfangreiche Literaturrecheche notwendig. Was nuetzt das beste Mikroskope, wenn das
      Praeparat schlecht ist. Dafuer ist jedoch eine ruhige Hand, Gedud und auch altes ein Stereomikroskop notwendig.
      Nur unter dem Stereomikroskop lassen sich vernuenftige Praeparate erstellen.
      Man soll sich seine Mikroskope aus alten Mikroskoper der Fa Zeiss oder Leitz zusammenstellen aber damit nicht zuviel
      Zeit verschwenden. Wer interessante Aufnahmen machen will, sollte sich den Dienst eines Elektronenmikroskopes leisten.
      Aber gerade fuer die Elektronenmikroskopie ist die Paraeparationtechnik und das vorbereiten des Objektes wichtig.
      So kann man fue etwa 50 Euro wirklich interssante Aufnamen erhalten die die Lichtmikroskopie
      in idealer Weise ergaenzen.

    • @lotharmayring6063
      @lotharmayring6063 10 หลายเดือนก่อน

      @@PUMAMicroscope ok. you are a seller of mikroskops therefore your mikroscope is the best
      but it is not th cheapest solution
      uses ergaval or axioscopes ar better

    • @lotharmayring6063
      @lotharmayring6063 10 หลายเดือนก่อน

      @@PUMAMicroscope y answers is
      1. do you think that zou are better than all zeiss developpers
      2. i simple need 3 microscops one is normal one is stereo and one is a REM oprtunity
      3. so i dont need a PUMA
      4. there is need for epi-fluorescenz. which only gives unsharp images
      epi is made by a simpe LED which can make oblique epi illmination is better
      5. as well you lean adpating many SHMPsy

  • @omarfarok5223
    @omarfarok5223 2 ปีที่แล้ว +1

    nice

  • @thecrazy8888
    @thecrazy8888 3 ปีที่แล้ว +2

    I could SWEAR I just heard a bunch of scope manufactures use curse words... the world is changing :D

    • @PUMAMicroscope
      @PUMAMicroscope  3 ปีที่แล้ว +1

      Nice. Democratisation of lab equipment should improve standards, promote accessibility and make prices fairer. 3D printing and open source are game changers.

    • @thecrazy8888
      @thecrazy8888 3 ปีที่แล้ว +1

      ​@@PUMAMicroscope After watching this... again, :P I have suggestions from lessons learned building 3D printers. To fix the vibration, you need to add MASS and Stiffness. Mass is easy: add weight OR (if you already took care of stiffness) you can screw it down on the desk or a 6x6 cement tile (which makes a nice base btw). But before all this you need to address all flexibility in the frame, make sure your parts are designed to resist flexing, add braces were required. Remember you can make internal modifications like adding round holes through a part, it adds a cylinder of stiff plastic through it. In addition to this, adding a case that attaches to all components would also fix stiffness. Maybe multiple 3D printed panels that would screw on to everything like an exoskeleton.

    • @PUMAMicroscope
      @PUMAMicroscope  3 ปีที่แล้ว +1

      Thanks, I really appreciate any efforts to help with the vibrations issue, which I consider to be due to the flexibility of the 3D printed plastic - especially joints - rather than a sheer lack of weight or flexibility of any individual component. However, if I can use a Trekism - 'I'm a doctor, not a mechanical engineer!'. All my mechanics (and 3D printing for that matter) are self taught so I am hoping someone cleverer than me can find a solution to this. The monocular tube you see is not a simple tube - if you look at the CAD file you can see it has internal baffles which strengthen it as well as block internal optical reflections. Also the QR mechanism already has internal hollow vertical tubes - as you suggested - but those can't strengthen the joint to the focus plate. My hunch is that a practical solution will be found with an external fixing frame (I did some orthopaedics so you can see how my mind works). It may be that such a frame must be made of metal (using off-the shelf fixings for practical adoption) with minimal 3D printed components. You can screw/bolt the feet to a wooden base or similar with the current design - but that won't fix the issue with the flexibility of the plastic connection to the focus plate. Also I am not sure how much the sprung focus mechanism contributes - but I have tried many variations on this during development and the solution you see is by far the strongest and most stable I was able to come up with. I am hoping that as more people build the scope they will tinker with various ideas and someone will come up with something that both works and is also practical for anyone to build (i.e. that doesn't require a fully equipped metal workshop with a 5-axis CNC lathe).

  • @perli216
    @perli216 3 ปีที่แล้ว +1

    dope

    • @PUMAMicroscope
      @PUMAMicroscope  3 ปีที่แล้ว +1

      You just taught me a new sense of the word - thanks :)

  • @testboga5991
    @testboga5991 9 หลายเดือนก่อน

    Kind of useless in the age of 200 dollar Chinese microscopes.

    • @Skunkhunt_42
      @Skunkhunt_42 8 หลายเดือนก่อน

      Still dope but yea i was shopping for a scope to today and was floored by whats avaliable for under 400. Any suggestions on bramds and where to buy?