Class (20) = Cultivation & Collection of Drug of Natural Origin | Methods of Cultivaton & Collection

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Cultivation of medicinal plant
Cultivation of medicinal plants requires intensive care and management. The conditions and duration of cultivation required vary depending on the quality of medicinal plant materials required.
Methods of Propagation:
Vegetative propagation (Asexual propagation): Vegetative propagation can be defined as regeneration or formation of a new individual from any vegetative part of the plant body. The method of vegetative propagation involves separation of a part of plant body, which develops into a new plant.
Methods of vegetative propagation:
They are two types:
1. Methods of natural vegetative propagation:
2. Methods of artificial vegetative propagation.
1. Methods of natural vegetative propagation:
a. Vegetative propagation by stem:Following examples: Runner: peppermint.
Bulb:Allium, Squill.., Tuber:Potato, aconite., Rhizome:Ginger and haldi.
b. Vegetative propagation by root: examples:Asparagus
2. Methods of artificial vegetative propagation:Various parts developed for natural vegetative propagation have also been used for artificial vegetative propagation.Following methods are used:
1. Cutting:
2. Layering:
3. Grafting:
4. Micro propagation: This method consists of growing cell, tissue and organ in culture. Small pieces of plant organs or tissues are grown in a container with suitable nutrient medium, under sterilized conditions. The tissue grows into a mass of undifferentiated cells called callus which later differentiates into plantlets. These are then transferred into pots or nursery beds and allowed to grow into full plants.
Collection of drugs
1. Medicinal plant materials should be collected during the appropriate season or time period to ensure the best possible quality of both source materials and finished products.
2. The best time for collection (quality peak season or time of day) should be determined according to the quality and quantity of biologically active constituents.
3. In general, the collected raw medicinal plant materials should not come into direct contact with the soil. If underground parts (such as the roots) are used, any adhering soil should be removed from the plants as soon as they are collected.
4. Collected material should be placed in clean baskets, mesh bags, other well aerated containers or drop cloths that are free from foreign matter, including plant remnants from previous collecting activities.
5. After collection, the raw medicinal plant materials may be subjected to appropriate preliminary processing, including elimination of undesirable materials and contaminants, washing (to remove excess soil), sorting and cutting.
6. The collected medicinal plant materials should be protected from insects, rodents, birds and other pests, and from livestock and domestic animals.
7. If more than one medicinal plant part is to be collected, the different plant species or plant materials should be gathered separately and transported in separate containers. Cross-contamination should be avoided at all times.
Time of collection:
The period of growth or development at which medicinal activity is highest has been carefully determined for many plants. The proportion, of alkaloid in the leaves of Hyocyamus Niger and of belladonna is largest at the beginning of flowering.
Example:
Stromonium leaves, gathered in the morning, contain a higher proportion of alkaloids than those collected in the evening.
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Unit 3 plant tissue culture padai

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