How to determine Potency (EC50 value) in Prism? | Step by Step | GraphPad Prism || Dr Junaid Asghar

More such lectures on data analysis and Pharmacology are available on my other TH-cam channel. Please visit:
Junaid Asghar PhD
/ @junaidasgharphd
GraphPad Prism 8 Tutorial for Researchers; finding EC50 value of a compound/ agonist.
How to construct a Concentration/ Dose Response Curve?
PN6047 (Novel Analgesic Compound for Chronic Pain; in Clinical Trials right now):
jpet.aspetjourn...
#Prism
#GraphpadPrism
#EC50

[@bartley7231]

I am so thankful right now. It took me three days to find this. I thought this IC50 thing was hopeless as I was not understanding the videos I saw and I need to submit my manuscript. Sir you are a lifesaver. It was well explained and I totally understand. Thanks a mil

[@DrJunaidAsghar]

Glad to know it was of help. Best wishes with the manuscript submission 👍

[@bartley7231]

@@DrJunaidAsghar How do I find the SEM values for the IC50 values?

[@DrJunaidAsghar]

Teachnically, you have to make curves for each experiment separately, get SEM for each experiment and then average it.

[@bartley7231]

@@DrJunaidAsghar Thank you

[@ilkemekici7232]

You saved my life. Thank you

[@sydneydallman741]

I would prefer to use log base 2 rather than log base 10 for my graph. How would I set this for analysis? Thanks

[@jyothialvares5668]

Thank you so much for the detailed video

[@negessemekonnen1854]

Nice tutorial, thank you.

[@abhigyanbhowmick9238]

Hi sir, hope this comment finds you well. Unfortunately when trying to find the EC50, the softare was giving me the option to insert it, as it was missing the option. I was hoping you could help?

[@InfinitiDreamerAkhshu]

hi sir, wny the concentration is in negative, is it neccessary to change into negative or should I keep in positive, when i select non linear regression, the column is empty, it is asking me to define everything. Please help me

[@Fahadofficial58262]

Great videos sirr

[@andresagat3744]

Thank you so much!

[@lauranunez6486]

Sir I have another question, when you already have the response in % do you still need to normalized it?

[@DrJunaidAsghar]

My response on Y-axis was not in %, therefore, I had to normalize it. If it's already normalized, you don't need to.

[@asmatsaqib1]

Thank u so much sir!!❤❤❤

[@michaelrestasuryayanuar237]

Sir, I wanna ask you, why u choose log (inh) vs normalized response- variable sloope instead of log(inh) vs normalized response?

[@DrJunaidAsghar]

Basically, the goal is to determine the IC50 of the inhibitor - the concentration that provokes a response half way between the maximal (Top) response and the maximally inhibited (Bottom) response. Many inhibitory dose-response curves have a standard slope of -1.0. This model does not assume a standard slope but rather fits the Hill Slope from the data, and so is called a Variable slope model. This is preferable when you have plenty of data points.

[@michaelrestasuryayanuar237]

@@DrJunaidAsghar oh, thank you, sir.. May I ask you again, you said earlier "plenty data points" how many exactly is the minimum data points to use this variable slope equation?

[@muhammadwaqarali5003]

Great sir❣️❣️❣️

[@ikseniksen8205]

Dear Dr. Asghar, I would like to ask how can we see the SD for these IC50 calculation?

[@DrJunaidAsghar]

For that you will have to analyze the "Normalize of your data" tab again (in the left side of the page).
Go to "Normalize of data", Click analyze, go to XY analysis, click "Row means with SD or SEM", press ok and there you go!

[@Alinochkaaaaaa]

Thank you! Can you tell me how to calculate CC50 using this guide?

[@DrJunaidAsghar]

For the cytotoxic investigations (Cytotoxic Concentration 50; CC50), we use exactly the same GraphPad Prism template as presented in this video. CC50 is the concentration of a compound decreasing the cell viability by 50% in comparison to control cells. I would suggest to use the same "Dose-Response - Inhibition" family of equations, and choose "log(inhibitor) vs. response - Variable slope (four parameters)". The IC50 or EC50 in prism is your CC50.

[@Alinochkaaaaaa]

Dear@@DrJunaidAsghar thanks for the quick response! You helped a lot

[@supremewrites9834]

Thanks sir 💓

[@muhammadmahmood2862]

Thanks sir g

[@sawsankhudairabbas-8930]

hello sir what is the name of the program in which the results are plotted

[@DrJunaidAsghar]

It's Graphpad Prism

[@sawsankhudairabbas-8930]

@@DrJunaidAsghar thanks, sir,

[@misbahmukhtar5602]

Tnxx sir

[@sarvenazghorbanian2751]

Hi sir,
I was wondering is it possible to label EC50 on the graph and know the associated log dose of it.

[@DrJunaidAsghar]

Yes it is possible. LogEC0 is also mentioned in the results section (pink page of the prism analysis where I obtained the EC50 of the compound).

[@sarvenazghorbanian2751]

@@DrJunaidAsghar thank you

[@ermiasm4824]

your email please