Ligation with T4 DNA Ligase

[@tiamheydari7729]

Thanks for the great video. Would you please share the reference for the formula?

[@StandardName562]

If you would use a PCR thermocycler, why not set the temp to 4 degrees and then up to 16, and then cycle down again for a few cycles? Wouldnt that give the best results..?

[@reubennjue7848]

My DNA is in TE buffer..Must I use water in my ligation reaction?