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Excellent explanation!! I was having lots of doubts regarding restriction digestion this video cleared my doubts completely!! You are a very good teacher! Thanks a bunch!
First time doing this experiment and I totally understand very, your explanation was fantastic
wonderful explanation
I have some questions:-Can you tell me the exact volume of all the reagents u used in this experiment including water and buffer?What is buffer 2.0?
Can you please help with the calculations; I have 20000 unit/ml restriction enzyme, how to calculate for 20 unit out of it?
what is the percentage of your agarose gel? I read that we need to have it at a higher concentration to see the separation
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Excellent explanation!! I was having lots of doubts regarding restriction digestion this video cleared my doubts completely!!
You are a very good teacher! Thanks a bunch!
First time doing this experiment and I totally understand very, your explanation was fantastic
wonderful explanation
I have some questions:-
Can you tell me the exact volume of all the reagents u used in this experiment including water and buffer?
What is buffer 2.0?
Can you please help with the calculations; I have 20000 unit/ml restriction enzyme, how to calculate for 20 unit out of it?
what is the percentage of your agarose gel? I read that we need to have it at a higher concentration to see the separation