You are a life saver. I struggled very hard to produce a report for this experiment because I didn't fully understand the meaning of the color change. The outcomes confused me a lot. I am incredibly grateful for this video.
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I think something is wrong in 3:57 minute.... if the bacteria IS recombinant (that means that has a LacZ genes) the bacteria is going to produce an enzyme to degradate Lactose (B-Galactoside). This enzyme is going to produce that lactose (which is white) converts into blue... because the Enzyme is going to react with the lactose (white) to separate the sugar of the X-Gal producing a blue color.... so the bacterias that has the recombinant DNA are going to be blue and those who has no recombinant DNA are going to be white.... is that correct?
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Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
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@@animatedbiologywitharpan you are welcome, ok, I have subscribed your channel on your special request, brother I also want to know about you, plz introduce yourself.
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In order to disrupt the lacZ gene via restriction digestion, there would need to be restriction site within the lac Z gene. Do we introduce the restriction site into the lacZ gene to perform the restriction digestion? If so, how does this not perturb the lacZ gene? If it does perturb the lac Z gene, then all colonies would appear white whether it was transformed with GoI or not. Can you explain this? Is there a section of the lacZ gene that is unimportant for transcription and thus the restriction site can be added there?
I have detailed and structured courses on biochem, molbio, biology techniques, cell biology and immunology.(Total price for all of this is 25 dollars) if you Wana try it you can use the following link and you can use my code AP10 to get 10% off...unacademy.com/plus/course/crash-course-on-molecular-biology-for-iit-jam-2021-gatb2020-252/2UZB2H60
Cells which are not transformed with plasmid cant be alive in the antibiotic containing media .....so no chance of getting blue colonies which corresponds to them
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You are a life saver. I struggled very hard to produce a report for this experiment because I didn't fully understand the meaning of the color change. The outcomes confused me a lot. I am incredibly grateful for this video.
Please share my channel link among friends and help me to reach big audience......do watch my other playlists as well ...might be useful
Watching this because a little confusion came up with this particular sub-topic. Thanks, your explanation really helped!
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My textbook did a terrible job explaining this, your video is very clear. Thanks!
Thanks a lot for this complement
An excellent illustration of the concept
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i have created entire mol bio playlist as well
I wasn't able to visualize this, but your video helped me a lot. Thank you so much :))
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I have watched my video on B galactosidase but u cleared all hats off to u
U r best
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Thanks !! You are gifted with understandable language and mind
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i wassss soo sooo confused, thank you so mucj for this
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Brilliant video! God bless you!
Please share among your friends
I just found your channel, and already love your illustrations and simplified explanations! Very very helpful👍👍👍👍Thanks for making this video!
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Superb explanation thankyou so much
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check. out our molbio playlist gor many related topics
Thank you for giving best explanation....🙏
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Outstanding video! you helped me for my exame.
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Mine too
Thank you so much! you just saved me before i take my exam
Han Lam please share my channel link with your friends and help me reach out big audience....also check out my playlist for more videos relevant for your studies
Thank you sir, day after tomorrow is my exam and this video helped me alot❤️
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@@animatedbiologywitharpan sure sir ❤️ love from Assam
Very useful video. Thanks a lot
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Thank you this was clear and to the point
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You are seriously life saver
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Thanks! This helped me understand this process for my research a lot!!!
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thank you, this made so much sense!!!
Please check out the entire molecular biology playlist th-cam.com/play/PLKtiwIJ8Q7rppT2_xeMbIbrfTvAttgVcP.html&si=pyRsypxDJ7nMHT80
Top class
this is very well explained thank you soooo much
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Very nice sir ...very useful
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Amazing
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Happy Teachers Day, Sir.🥰
Thanks a lot for wishing me...please share my channel link with your friends and help me to reach big audience
Thank you very much!
Subscribed and shared!! Great video to understand screenable markers.
How do you make the powerpoint so good?
I have a detained course on how to make ppt like this . It's low cost if interested please let me know. Email us at arpanparichha1994@gmail.com
I think something is wrong in 3:57 minute.... if the bacteria IS recombinant (that means that has a LacZ genes) the bacteria is going to produce an enzyme to degradate Lactose (B-Galactoside). This enzyme is going to produce that lactose (which is white) converts into blue... because the Enzyme is going to react with the lactose (white) to separate the sugar of the X-Gal producing a blue color.... so the bacterias that has the recombinant DNA are going to be blue and those who has no recombinant DNA are going to be white.... is that correct?
Appreciate your effort 👌
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thanks !
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
It was perfeccccccct thank you
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@@animatedbiologywitharpan yeah sure
thanks
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Thank you very much! Helped a lot
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Thanks a ton . It was really helpful
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
thank you very much for this video , keep up the good work !!!
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Really helpful. Thank you so much
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very good, very informative.
Muhammad Bilal Bhatti thanks and please subscribe to my other channel th-cam.com/channels/4IpyopsGWSjaPACNTZLuqg.html
@@animatedbiologywitharpan you are welcome, ok, I have subscribed your channel on your special request, brother I also want to know about you, plz introduce yourself.
Visit the about section of my channel
@@animatedbiologywitharpan ok, thanks.
Thank youu
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Thank you sir .. it was helpful
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Plz help me what word do you used at 2:55 while talking about multiple cloning site
Perturbed
Thank youuu so much.. it is so clear..😍
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It's structure or functional based recombinant bacteria analysis sir
I could not undetstand your question , please elaborate
In this technique of recombinant analysis of bacteria... Bacterial Functional based analysis or bacterial structure based analysis
Thanks a lot! It's so helpful
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Thank you so much
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great job .
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@@animatedbiologywitharpan sure .
Very useful vedio! 👍👍
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In order to disrupt the lacZ gene via restriction digestion, there would need to be restriction site within the lac Z gene. Do we introduce the restriction site into the lacZ gene to perform the restriction digestion? If so, how does this not perturb the lacZ gene? If it does perturb the lac Z gene, then all colonies would appear white whether it was transformed with GoI or not. Can you explain this? Is there a section of the lacZ gene that is unimportant for transcription and thus the restriction site can be added there?
The restriction site which is introduced in it is called polylinker. It is so small that it doesn't really affect the lacZ gene..
Thankyou very much sir
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thankyouuu very michh🥰🥰🥰
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very nice video
thank you
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Thank you so much❤❤❤
Please share my channel link with your friends and help me to reach big audience
I have detailed and structured courses on biochem, molbio, biology techniques, cell biology and immunology.(Total price for all of this is 25 dollars) if you Wana try it you can use the following link and you can use my code AP10 to get 10% off...unacademy.com/plus/course/crash-course-on-molecular-biology-for-iit-jam-2021-gatb2020-252/2UZB2H60
Nice explanation
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Good one
Bro u don’t explain about growing those rec bacteria in agar plate in presence of x-gal
Thanks for this suggestion. It will help us to improvise our content
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Perfect explanation. Thank you Arpan! :D
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I have another channel which you can subscribe as well th-cam.com/channels/4IpyopsGWSjaPACNTZLuqg.html
Really usefull vdo😘
If you like my explanation please share among your friends and help me to reach big audience
@@animatedbiologywitharpan sure
thanks a lot, that was very helpful. 🙂🙂🙂
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Thanks from Chinese
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Amaaaazing thank you so much
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thank you !
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Arpan can u tell me important topics from analytical Techniques which is necessary for tifr and iitjam
Mendelian genetics, NMR, bacterial growth curve, enzyme kinetics, cell signaling.
Do non transformed host cells can produce blue colonies on x-gal medium???
Cells which are not transformed with plasmid cant be alive in the antibiotic containing media .....so no chance of getting blue colonies which corresponds to them
th-cam.com/channels/4IpyopsGWSjaPACNTZLuqg.html do subscribe to my other channel
Tq 😃😃
can you please share your slides?
slides are not available...sorry
@@animatedbiologywitharpan its okay...no problem
Helpful
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Good
Superb
th-cam.com/channels/4IpyopsGWSjaPACNTZLuqg.html please subscribe to my other channel
Please discuss about buffers
krishna kumar could you be bit more specific
@@animatedbiologywitharpan topic of biochemistry in IIT jam syllabus not specifically know about buffer
Pls help
X-gal bring me here
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v.nice thankyou
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Thank u bro....
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@@animatedbiologywitharpan sure