Urine Protein Estimation: Simple and Effective Heat Coagulation Test

Heat coagulation Test is routinely used to screen for proteinuria in pregnancy. Small quantities of albumin are normally filtered at the glomerulus and reabsorbed by the proximal tubule. The protein that escapes reabsorption does not exceed 150 mg/24 hr so that proteinuria in excess of this amount is regarded as pathologic.
Test Methods:
Qualitative tests
Heat coagulation test
Hellers nitric acid test
Sulphosalicylic acid test
Quantitative test
Esbach’s albuminometre test
Other tests
Protein reagent strip test
Biuret test
Urine protein electrophoresis
Heat coagulation Test:
Advantage - cheap test which does not require technical expertise
Disadvantage - specific type of proteinuria cannot be judged