Lac Operon

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  • เผยแพร่เมื่อ 24 ธ.ค. 2024

ความคิดเห็น • 113

  • @Amiinerz
    @Amiinerz 11 ปีที่แล้ว +22

    this is easily the best, and most informative, video on the lac operon

  • @gauravgirishsadhnani
    @gauravgirishsadhnani 11 ปีที่แล้ว

    Probably the best Lac Operon explanation on youtube. Thank you!

  • @the_jacked_doc
    @the_jacked_doc 11 ปีที่แล้ว

    Best explanation out there on lac gene expression. Covers both positive and negative control. Thanks bruh.

  • @SpyralDC1
    @SpyralDC1 12 ปีที่แล้ว

    best video for students to understand the operation of the lac operon. thank you.

  • @VyNguyen-gl4ws
    @VyNguyen-gl4ws 10 ปีที่แล้ว +1

    This is the best video explains how Lac Operon operates. Thank you for your work!

  • @armandohasudungan
    @armandohasudungan 12 ปีที่แล้ว +1

    nicely presented~

  • @iamgweedo
    @iamgweedo 13 ปีที่แล้ว

    I like the simple animation! It helps to see it in motion!

  • @AsbatHasanChannel
    @AsbatHasanChannel 12 ปีที่แล้ว

    After cAMP becomes bound to the CAP to form a complex, it goes on to attach to a specific site on the lac operon of the gene called the "CAP site". This enhances the affinity of the gene to the RNA polymerase, which can now attach and transcribe the mRNA much more efficiently.

  • @Sree4uall
    @Sree4uall 13 ปีที่แล้ว

    this really gave me a quick review of lac operon model i surely suggest this one over manyother videos on this topic!! thanx a lot!!

  • @MsKievStar
    @MsKievStar 12 ปีที่แล้ว

    Great detailed explanation with no distractions. Thank you

  • @BonanzAA
    @BonanzAA 12 ปีที่แล้ว

    I'm studying for a test and this video helped me A LOT! THANKS Johnny!

  • @reyescristy1988
    @reyescristy1988 11 ปีที่แล้ว

    Sigh...videos like this make me so relieved after studying for hours and being hours away from a midterm :o

  • @lisagomez3613
    @lisagomez3613 14 ปีที่แล้ว

    This is a great video!!! Great for explaining what my genetics teacher can't.

  • @03wychaidar
    @03wychaidar 13 ปีที่แล้ว

    I have an exam in 2 hours and this has probably just saved me!!!

  • @johnnyclore1571
    @johnnyclore1571  12 ปีที่แล้ว

    Although B-galactosidase transcription will not be upregulated, it is true that a very small amount of transcription will occur even in the presence of an active repressor. This is because repressor binding is governed by kinetics describing the probability of binding or falling off the promoter. The bound & unbound states will be in an equilibrium determined by the equilibrium dissociation constant, which favors binding, but allows for low level transcription even with an active repressor.

  • @candy4lifeeeeee
    @candy4lifeeeeee 10 ปีที่แล้ว

    God bless your teaching skills. Saved me from my test. Well, nearly a quarter of it.

  • @poincareconjecture5651
    @poincareconjecture5651 7 ปีที่แล้ว

    Well done Mr. Clore!---especially the positive control of the CAP protein triggered by cAMP when glucose levels are low---i get the picture now:)

  • @vesrangej934
    @vesrangej934 2 ปีที่แล้ว +1

    Great 👌 information...

  • @Longhairbeware86
    @Longhairbeware86 11 ปีที่แล้ว

    Thank you so much for this. You are better than my college professor.

  • @Jonesle90
    @Jonesle90 12 ปีที่แล้ว

    Just for clarity, does CAP (with cAMP bound to it) bind to the RNA polymerase which then binds to the promoter and starts transcription? or in other words, what exactly does CAP bind to that facilitates transcription?

  • @joeramirez2156
    @joeramirez2156 5 ปีที่แล้ว

    So simplistic to understand. Very well done. Thank you.

  • @avinashthumma
    @avinashthumma 13 ปีที่แล้ว

    that was to the point!! absolutely specific...awesome...it has relived my confusion of a week in jus 4 min..thank you sir..

  • @rgmac1993
    @rgmac1993 12 ปีที่แล้ว

    Bravo! Excellent information! It's not as pretty as other videos, but it has the info others seem to forget(Cap protein and cAMP molecule)!

  • @schoi567
    @schoi567 11 ปีที่แล้ว

    I know this might be unrelated to the video,but I have a question regarding bio 142.
    I wonder about lac and trp repressor mutants. When lac operon repressor or trp operon repressor gets mutated, does it mean they those repressors will not be able to bind to the operon? Or does it mean that lactose trp will not be able to bind to the repressors? Or can it mean both?

  • @katiedidier479
    @katiedidier479 9 ปีที่แล้ว

    Isn't glucose the molecule on the right at 33 seconds?
    The 4' spots are reversed for galactose and glucose, right?

  • @DirectorAntify
    @DirectorAntify 8 ปีที่แล้ว +1

    Best explanation I heard so far, thank you!

  • @TheLuchador1988
    @TheLuchador1988 10 ปีที่แล้ว

    This really clarified a seemingly difficult concept, thank you very much.

  • @winner322
    @winner322 14 ปีที่แล้ว

    Hey can i ask a question?
    What's the difference between the lac promoter and the lac operator. Kinda confused about the uses for each of them.

  • @xlaunamx
    @xlaunamx 8 ปีที่แล้ว +1

    Best video on Lac Operon

  • @keroro28
    @keroro28 12 ปีที่แล้ว

    THANKYOU SO MUCH. I WAS STRUGGLING FOR HOURS.

  • @venster552
    @venster552 14 ปีที่แล้ว

    Thanks a million!! Perfect summary just before my test!
    God bless!

  • @zombiesniperify
    @zombiesniperify 12 ปีที่แล้ว

    Great video man, it really clears things up. Go ahead, have my thumbs up.

  • @bekahshupe
    @bekahshupe 9 ปีที่แล้ว +3

    Love this video. It was short and sweet. My kind of video.

  • @TeamFresh182
    @TeamFresh182 13 ปีที่แล้ว

    this video saved my ASS!! this was the best video on the lac operon!

  • @johnnyclore1571
    @johnnyclore1571  14 ปีที่แล้ว

    @crazychic0009 with no lactose and plenty of glucose, B-galactosidase would NOT be produced; it would be unnecessary given that glucose will be used as the primary sugar source. Glucose is a monosaccharide (unlike lactose which is a disaccharide) and therefore does not need to be cleaved by an enzyme like B-galactosidase for it to be used by the cell.

  • @yjaygallardo361
    @yjaygallardo361 11 ปีที่แล้ว

    excellent video. TH-cam makes studying so much easier.

  • @dadipoli
    @dadipoli 12 ปีที่แล้ว

    I thought i was the only one looking up biochem videos. Biochem is the worst, doctors in training goes through it so fast. Good luck on yours.

  • @drsaniavlogs
    @drsaniavlogs 9 ปีที่แล้ว

    Video for transcription nd translation are available

  • @tedboo
    @tedboo 12 ปีที่แล้ว

    Thank you thank you thank you! Exam today and I forgot to bring my notes for quick revision.

  • @hollyflowerrose
    @hollyflowerrose 13 ปีที่แล้ว

    @theauk
    This is not an entirely true response. Remember that even though the repressor molecule prevents the lac operon from being transcribed, it sometimes "pops" off the operator (in reality the repressor is mostly bound, pops off, rebinds, pops off, rebinds, etc..... it does NOT just stay bound the entire time). During the small amount of time it pops off, RNA polymerase transcribes a small amount of genes- hence there is always a small amount of B-galactosidase and permease in the cell.

  • @StreitMark
    @StreitMark 12 ปีที่แล้ว

    I heard that even without lactose, so with an active repressor, a small transcription occured. Anyone heard of that? Can someone explain? thank you

  • @benjaminjonik55
    @benjaminjonik55 11 ปีที่แล้ว

    Great, concise video. Really helped me grasp the topic. Thank you

  • @ballr41
    @ballr41 14 ปีที่แล้ว

    this is a very good video man, its helped my understanding alot
    Thanks

  • @CHRISTINAAx345
    @CHRISTINAAx345 12 ปีที่แล้ว

    What about when only glucose is present?

  • @Briony28
    @Briony28 11 ปีที่แล้ว

    just helped me through a chapter of revision :) thanyou so much i actually understand it so much better now!!! :)

  • @aainaanadiabintimohammedha957
    @aainaanadiabintimohammedha957 7 ปีที่แล้ว

    May I know what app do you use to make this video?

  • @xrayspex7341
    @xrayspex7341 11 ปีที่แล้ว +5

    I'm not trying to be dense here....But i'm struggling to understand what is happening in thre presence of glucose. I understand that its preferable but it seems like the structural genes are being produced even in its presence....

    • @johnnyclore1571
      @johnnyclore1571  11 ปีที่แล้ว +14

      Regardless of glucose levels, if lactose is not present, the transcription of the structural genes is inhibited by the binding of the repressor to the operator. Assuming lactose is present and the repressor inactive, transcription of structural genes will depend on the presence or absence of glucose. If glucose is present, then cAMP levels are low and therefore CAP remains inactive and RNA polymerase doesn't efficiently bind the promoter, so transcription of the structural genes is low. If glucose is absent, cAMP levels increase, activating CAP and enhancing RNA polymerase binding. This leads to transcription of the structural genes at high levels only if lactose is present and glucose is not.

    • @Hehehihi76
      @Hehehihi76 6 ปีที่แล้ว

      +Johnny Clore y r u here
      ....like u know every thing...😶

  • @MsBubblyshe
    @MsBubblyshe 11 ปีที่แล้ว

    Thank you :) saved me tons of hours in reading!

  • @utoobluver4ever
    @utoobluver4ever 12 ปีที่แล้ว

    Thank you so so so so so so so much this was an amazing explanation.

  • @geandily
    @geandily 13 ปีที่แล้ว

    Some of this CAP stuff is apparently wrong according to my Bio Professor, glucose apparently blocks LacY(the transport protein for lactose) and so lactose is unable to enter the cell.
    CAP is only part of why lactose is not transcribed in the presence of glucose.
    Also: remember that basal(very low) levels of the lac operon are being transcribed at all times, even when the repressor is bound to the operator.(lacI)

  • @alirocksize
    @alirocksize 10 ปีที่แล้ว +1

    thank you! but why dont u make i video about the trip operon

  • @deepikayadav141
    @deepikayadav141 11 ปีที่แล้ว

    ohhh bt in presence of glucose ,the level of cAMP decreases but u r saying that cAMP level increases in presence of glucose.plz let me sure about it .

  • @marymlak12
    @marymlak12 11 ปีที่แล้ว

    I hated lac op topic for the MCAT before, but this video really helped me make sense out of that sucker

  • @HES7183
    @HES7183 10 ปีที่แล้ว +1

    Very well explained. Thank you.

  • @Mounika1128
    @Mounika1128 14 ปีที่แล้ว

    Thank you, very well explained and demonstrated!

  • @kamatmehbro
    @kamatmehbro 10 ปีที่แล้ว

    Champion! thanks so much! very clear and concise :) Much appreciated!

  • @xdabomx
    @xdabomx 13 ปีที่แล้ว

    This was sooo helpful. Thank you for the upload!

  • @firstclassartists
    @firstclassartists 11 ปีที่แล้ว

    wow, awesome video man!!!

  • @2012farfar
    @2012farfar 6 ปีที่แล้ว

    Great explanation, thank you!

  • @Nosa423
    @Nosa423 12 ปีที่แล้ว

    very good job

  • @TheToxicMegacolon
    @TheToxicMegacolon 6 ปีที่แล้ว

    Awesome animation

  • @mattd3377
    @mattd3377 11 ปีที่แล้ว

    RNA polymerase can't even bind the promoter at all when the repressor is attached to the operator due to steric inhibition**

  • @saktivelann
    @saktivelann 11 ปีที่แล้ว

    Great video man!

  • @kmsr007
    @kmsr007 11 ปีที่แล้ว

    good concept

  • @talaldubi7071
    @talaldubi7071 9 ปีที่แล้ว

    great video !!! thank you it helped me a lot

  • @PalestineVioletRose
    @PalestineVioletRose 12 ปีที่แล้ว

    Thank you very much!!!! This video helped me lots!

  • @TheJavoFlava
    @TheJavoFlava 13 ปีที่แล้ว

    Thank you. This video really helped.

  • @zacheryryenrobinson523
    @zacheryryenrobinson523 9 ปีที่แล้ว

    Thank you very much! great video!!

  • @mattymx21
    @mattymx21 12 ปีที่แล้ว

    i wish you were my molecular genetics lecturer..

  • @kookaaw
    @kookaaw 11 ปีที่แล้ว

    YOU ARE AWESOME ! NOTHING TO ADD

  • @ImNicoEh
    @ImNicoEh 10 ปีที่แล้ว

    You are the best! That helped a ton!

  • @lexa4391420
    @lexa4391420 13 ปีที่แล้ว

    very helpful video!

  • @TheGreenHoodieGirl
    @TheGreenHoodieGirl 11 ปีที่แล้ว

    *Falls to knees*
    IT MAKES SENSE!!! Now I won't fail genetics.

  • @TheFoshizzler
    @TheFoshizzler 12 ปีที่แล้ว

    Great appreciated, man. Thank you very much.

  • @zombiesniperify
    @zombiesniperify 12 ปีที่แล้ว

    No, he meant even though lactose is present. The structural genes aren't produced unless lactose is present. But there ALSO needs to be low levels of glucose, which is the whole point of this video. So he's saying yes even though lactose is present, the genes won't be produced BECAUSE there are high levels of glucose (low cAMP) :)

  • @erGurpreetsnanda
    @erGurpreetsnanda 14 ปีที่แล้ว

    nice work thanx

  • @7la557
    @7la557 12 ปีที่แล้ว

    God Bless You ..YOU SAVED ME!!

  • @MrAntihumanism
    @MrAntihumanism 11 ปีที่แล้ว

    brilliant

  • @kimmyfly52
    @kimmyfly52 11 ปีที่แล้ว

    Very good thank you

  • @Mojoelmo
    @Mojoelmo 14 ปีที่แล้ว

    Thanks, very informative

  • @michellekraus5937
    @michellekraus5937 11 ปีที่แล้ว

    Thank you for this video!!!

  • @omarfawzi8802
    @omarfawzi8802 12 ปีที่แล้ว

    شكرا لك :)

  • @nsrgsp6225
    @nsrgsp6225 8 ปีที่แล้ว

    Thank you very much sir

  • @TGIFtomorrow
    @TGIFtomorrow 12 ปีที่แล้ว

    THANK YOU

  • @nrazdan8
    @nrazdan8 12 ปีที่แล้ว

    GOD BLESS YOU.

  • @elisabethpancakes
    @elisabethpancakes 12 ปีที่แล้ว

    Dudeeeeeeeeeeeeee you are like the best ever!

  • @winner322
    @winner322 14 ปีที่แล้ว

    @marinarina113
    Thanks a lot!! I understand how it works now.
    =]

  • @kalpana142
    @kalpana142 13 ปีที่แล้ว

    thanks!

  • @GameGatsby
    @GameGatsby 10 ปีที่แล้ว

    thank you for the video it helped me a lot

  • @rupeshkumar2020
    @rupeshkumar2020 13 ปีที่แล้ว

    thank u very much,

  • @bigdon1281
    @bigdon1281 11 ปีที่แล้ว

    Thanks so much. Really helpful :)

  • @redon781
    @redon781 13 ปีที่แล้ว

    thank you so much!!!

  • @saresaurus
    @saresaurus 10 ปีที่แล้ว

    THANK YOU!

  • @uberbeamerman
    @uberbeamerman 12 ปีที่แล้ว

    Legend, thank you so much!

  • @dianelam6607
    @dianelam6607 11 ปีที่แล้ว

    thank you very much! this was very helpful! :D

  • @amymay16
    @amymay16 8 ปีที่แล้ว

    Thank you this helped!!

  • @MEHAKMT
    @MEHAKMT 13 ปีที่แล้ว

    gud video

  • @bfurl69
    @bfurl69 12 ปีที่แล้ว

    ok, i got it now. thanks

  • @ko88mm
    @ko88mm 13 ปีที่แล้ว

    thank u thank u thank u.....sooooo much...

  • @farahkhashan3479
    @farahkhashan3479 11 ปีที่แล้ว

    THANK YOUUUU!!