Plasmid Extraction from E.coli Bacteria

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  • เผยแพร่เมื่อ 22 ธ.ค. 2024

ความคิดเห็น • 2

  • @parks7165
    @parks7165 ปีที่แล้ว

    This is very helpful. I have been using NEB's kit with JM109 cells and always have floating particles (cell debris) with the pellet after the neutralization of the lysis. Any suggestions to avoid this, or cause? Do your debris pellet well with this Qiagen kit? I am always getting genomic DNA contamination

    • @virologylab5956
      @virologylab5956  11 หลายเดือนก่อน

      Try to remove the growth medium (LB or other liquid media you used) perfectly. Then add extraction kit. Sometimes growth media remains in the tube (after centrifuze) and reduces the efficiency of kit. Hope you will not find floating cell debris.