This is very helpful. I have been using NEB's kit with JM109 cells and always have floating particles (cell debris) with the pellet after the neutralization of the lysis. Any suggestions to avoid this, or cause? Do your debris pellet well with this Qiagen kit? I am always getting genomic DNA contamination
Try to remove the growth medium (LB or other liquid media you used) perfectly. Then add extraction kit. Sometimes growth media remains in the tube (after centrifuze) and reduces the efficiency of kit. Hope you will not find floating cell debris.
This is very helpful. I have been using NEB's kit with JM109 cells and always have floating particles (cell debris) with the pellet after the neutralization of the lysis. Any suggestions to avoid this, or cause? Do your debris pellet well with this Qiagen kit? I am always getting genomic DNA contamination
Try to remove the growth medium (LB or other liquid media you used) perfectly. Then add extraction kit. Sometimes growth media remains in the tube (after centrifuze) and reduces the efficiency of kit. Hope you will not find floating cell debris.