Determination of Crude Fat Content (Soxhlet Extraction) _ A Complete Procedure (AOAC 2003.05)
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- เผยแพร่เมื่อ 19 ก.ค. 2020
- Determination of Crude Fat content is a common proximate analysis. This parameter is very important for the analysis of food and feed samples. This video represents the detailed procedure of Crude Fat analysis using Soxhlet Apparatus according to the Method of AOAC 2003.05.
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#Fat
#Proximate
#Crude
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It was very helpful in the experiment learning and making a good understanding! Thank you sir!
It's my pleasure. Thanks for being with me.
Thank you so much it was very well explained 😄
Nice Video and Clarity of the Analysis.. Thanks...
You are welcome
Great thanks for clearing my concept
Thanks
Love from India 😍 thanks for this explanation 🙏
Thanks
IT WAS HEALP FULL AND GOOD UNDERSTANDING EXPERIMENT
Thanks
Your videos are really very helpful to understand the process thanku for it🍫🍫🍫😊😊
Most welcome 😊
Thank you so much, keep going
It's pleasure. Thanks for being with us.
Thankyou sir it helped me lot for my test...
You are most welcome. Stay with us.
Thank you sir,
Very detailed
Thank you for being with us.
Woow this explained greatly thanks you very much
You are welcome
It's very useful for me....Thanks
Stay with us
very nice video sir i fully understood it thank you
Glad to hear that
very nice video thanks for the help....
Krishna Gorkha, Thanks. Be with us.
Its very helpful for me thank you so much 💞
Thank you
A well-organized illustration. But I would definitely suggest you slow the background music. It makes enough contrast with your own voice. Thank you.
That you so much.
Hi, thanks for the nice video, it is very illustrative. Nevertheless, I have a question: the method actually calls for the Randall modification of the standard Soxhlet extraction to perform the assay, which implies submerging the sample into boiling solvent, (in this case n-hexane). Is it correct to perform the assay with a regular Soxhlet extractor, as shown in the video? Any comment will be helpful. Thanks!!!
Yes. We followed the AOAC 2003.05 official method strictly to make this video which is applicable for regular Soxhlet extraction of different solid samples.
Anyways, thanks for your comment. Stay with us.
clear explanation 👌👌
Vidya Ammu, thank you for your comment. Be with us.
Nice video
Please add explanation of chemical reagents why we use it
And make another video related to food chemistry
Thank you
Thank you so much for your observations. We are already working to make videos explaining the reaction mechanism of every test we did. Our new video will be uploaded very shortly. Stay with us.
Weight of sample is onlyy the weight of powder or also the weight of filter thimble?????
Fantastic Bro .... S helful for viva
Thank you so much. Stay with us.
Nice explanation
Thank you.
Thank you i benefit this vedio more experience and knowledge
Thank you so much. Stay with us.
@@MicroChemsExperiments ok always I'm with you to get knowledge
@@fadumoladan Thanks
Good explanation
Keep watching
Thanks for the video. Would like to ask isn't the weighing of the sample actually including the weight of a paper-made thimble even though you tared the weight of the thimble prior to weighing?
No. If you tare the thimble weight, then the thimble weight will not be counted. Only sample weight will show in the balance machine
@@MicroChemsExperiments hi goodday! i would like to ask, you said in the video that "sample weight varies depending on the expected fat %", Can you explain further? and I would like to ask what would be the sample weight for rice grains? thank you for answering
it was a great one, easy to understand, thank you.
Stay with us.
Well explained
Thanks
Thank you so much
I have one question. What temperature do I set the heating mantle at for n hexane?
130 to 150 degree Celsius.
This is a really good video with clear content. But I have to add the music choice was way too catchy. Anyway loved both. Thank you very much for uploading this.
Thank you so much for being with us.
clear explanation
Nada Naji Tawfeeq,
Thank you for your observations. Be with us.
👍👍👍👍👍welllllll explained💯💯💯💯💯💯💯
Glad you liked it
Thank you for this video. Can I re-use the wastage n-hexane and thimble holder?
Yes. You can re-use wastage hexane and thimble holder.
@@MicroChemsExperiments thank you very much for your quick reply.
@@princessasuelimen8358 you are most welcome. Stay with us.
Thanku so much Sir ✨🔥
Anytime
well done
Thank you
hello sir. can i extract chicken feet skin using this apparatus? or any related animal waste?
How do you calculate/double-check the fat content with the nutritional information at the back of a products packaging?
Take help from other sources
thanks nice video
Thanks to you too
Thank u soo much sir u r video proved to be a helpful mean for me 😊😊
You are welcome.
Great video as well! Can I know does this method suitable for determination of fat in liquid goat milk?
No. This method is not for milk sample. Watch this video for milk fat determination: th-cam.com/video/muswKsqvGnM/w-d-xo.html
Thank you for a video
But i have a question instead of hexan i use petroleum either what temperature i should set it on for solvent, and for how long , and how many circles is better for extraction sesame oil?
You can use petroleum ether. Same temperature and time should be followed as shown in this video.
@@MicroChemsExperiments i used it today but i don't know why in 200 c just 3 cycle in hour happened
@@sheraz_zuher It should not be happened because the boiling point of petroleum ether is 60-80 degree Celsius.
Please use better grade of petroleum ether from a reliable source. I hope your problem will be solved.
Can i use this method for coconut milk
I never miss watching your new video. You do a good job. However, I have a query. Could you please clarify for me the parameter name of Oil Content in oilseed and Total Fat/Crude Fat in oilseed are the same or not? If it is the same then can I follow your procedure? And if it is not the same, can I get the exact procedure from you. Thank you for your earliest feedback.
Same procedure. Please follow crude fat extraction method given in this video
can the fat layer be washed?
how to perform fat analysis of semi solid food samples? like Hummus/Cheese spreads or Dips? please guide on the same
In future, we will develop video later
Please make video of beeswax extraction so that extract can be used for antimicrobial activity.
At what percentage of crude fat considered high?
hi what temp should be provided in case of using heating mantle
used to keepflask
120 degree Celsius
Hi, can we just let the moisture in the flask evaporate on its own instead of using an oven?
Thanks.
@Raghaban619, You can not evaporate the moisture and remained n-Hexane completely by keeping the flask at room temperature. Because n-Hexane and moisture will remain in the mixture form with the oil content in the flask. You must apply heat to remove misture and n-Hexane from the flask completely.
@@MicroChemsExperiments Got it! Thanks for replying.
@@Raghavan619 you are most welcome
Can you make video about DNS Assay for Determination of reducing sugar?
Ok we will make. Stay with us.
Hi, can i know how many gram of sample should i use if im using predried homogenised fish muscle as a sample? Is it still 4.5g?
Yes. Sample amount is same
Hey, i had a question, does the sample need to be moisture free before putting it in thimble ?thanks in advance
No need...
No need
Can i use the n-hexane collected from thimble again for another crude fat extraction?
Yes. You can
thanks a lot for your explanation it's very clear. Could you tell me please the temperature of the extraction process? during the 6 hours
Mahmood Hashem, Set the temperature in such a way that facilitate the reflux rate of 5 drops per second. Usually, 100-150 degree Celsius is used for the Fat extraction. Than you for being with us.
@@MicroChemsExperiments thank you so much for your rapid response , may I ask another question; can we use standard hotplate instead of heating part in the soxhelt appartus?
@@MahmoodHashem Thanks for your second question. In case of hot plate, it will heat only flat bottom part of the flask and only Hexane of the bottom layer will be evaporated at a particular time. But in the soxhlet apparatus, it heats on the maximum part of the flask, as a result evaporation of Hexane from all side of the flask will occur which is much effective way for Fat extraction.
So, if you don't have Soxhlet Apparatus facilities, you can use Hot Plate temporarily but it is not recommended for permanent use.
@@MicroChemsExperiments great illustration thanks so much🙏
@@MahmoodHashem you are most welcome. Be with us
Exelent work done .
Pls testing oil and grese in inlet etp water. Or give me a link
Mohd Shariq, thank you so much. Which test do you want? Please specify.
I am using diethyl ether solvent for crude fat extraction. But the solvent is coming out of the condenser. What is might be the issue?
Don't use diethyl ether in soxhlet extraction. You can use petroleum ether replacing the n-hexane
What is the importance of using hydrochloric acid in fat determination analysis?
Cozy Cove Hamstery, thanks for your query
Please take a look at the principle of fat extraction of a solid sample. Normally a dried, ground sample is extracted with n-Hexan which dissolves fats and oils of the sample. During extraction, in the Soxhlet Apparatus, n-Hexane dissolves fats and oils of the sample and carries into the Round bottom flask. In this way, the extraction of fat continues for 6 hours by refluxing the n-Hexane (Oil free n-Hexane goes up by evaporating and after condensation it falls on the samples and get down to the flask carrying fats & oils from the sample). After 6 hours, the n-Hexane is then evaporated from the flask to separate it from the oil. The resulting residue (Fats & Oils) is weighed and referred to as ether extract or crude fat.
So there is no need to use hydrochloric acid (HCl) in this fat extraction procedure.
Hi, can you please tell me the exact temperature..?? I'm applying this method but it doesn't boil and takes too much time.. I also used hexane and my plant is Monstera deliciosa.. Please help me with this..??
Thank you in advance..
Increase your temperature to about 130 degrees Celsius to start boiling fast.
@@MicroChemsExperiments okay thank you so much..
How much temperatute is used
Sir thanks for the video...u have used grounded powder form how can we determine if it is not in powder form eg:to determine the fat of raw spinach containing90% moisture???
Blend the sample and after that dry it using Hot Air Oven. Test the dried sample for fat analysis following this video
Is it not possible without drying it??? What if we use raw without drying??
@@ujjwalkoirala6770 You can try raw but drying is better
@@ujjwalkoirala6770 In the case of fat extraction, the sample must be dry. If you want the result from the original sample, then you have to:
- Weigh in the original sample, dry it and then extract it or,
- Weigh in the original sample, rub in sodium sulphate and then extract (* both remove the water😉).
its a nice presentation. but how much of solvent we use?
It is not the exact amount. please use enough for reflux condensation
The hexane solvent recovery will be 100% or bless after each process?
Loss of solven is occurred because of the evaporation in different steps
Good evening, why we using hexane in fat extraction?
Because fat is dissolved in hexane
Thnkuuuuu
You are welcome
What method can I use to determine the crude fat in honey samples?
You need different method of test. We will upload video later. Stay with us.
Thank you, and for reducing sugars and sucrose contents as well please
@@Julz_Naturals Noted
please, explain the greens inside the condenser...
That was algae growth because of warer flow inside of the condenser
Can u please explain what is the difference between crude fat and fat, crude protein and Protein and crude fibre and fibre?
Please find your answere from google. Thank you
HI ! can we recover the solvent using water bath method for next run? instead of hot air oven
Yes you can
@@MicroChemsExperiments but for the temp and timer is same with hot air oven or not ?
@@nursyazwani6680 same
@@MicroChemsExperiments okey thankyouu
Can we reuse the cellulose thimble after the extraction?
Yes. You can
@@MicroChemsExperiments Thank you. What kind of filter paper do you use for this analysis?
Hi, Can I ask you one doubt.For all samples the extraction time and temperature same ?What is the time and temperature for extraction if we are using petroleum ether.Kindly please reply.Thank you.
Same for all sample and petroleum ether
@@MicroChemsExperiments For sample weight 5g Temperature 110 degree and time 3 hrs is okay for extraction ?
@@sandhyaranjith427 ok
I wanted to do analysis for honey
Can I use this same method (but you mentioned only dry sample)
Plz suggest
No. You can't analysis fat of honey by this method.
Which sample ?
Instead of evaporating the solvent in Hot Air oven, can we recover the solvent using vacuum rotary evaporator for the next run
Yes. You can use rotary evaporator to evaporate the solvent.
@@MicroChemsExperiments What's the procedure for that,will you think the fat will be isolated in the receiving flask
@@SourodiptoOfficial First, extract the fat from your sample using the Soxhlet Apparatus. Then evaporate the solvent using Rotary Evaporator. Finally you will get pure Fat in the flask. Now take wright and calculate the fat% as done in this video.
@@MicroChemsExperiments Thanks for replying to my comments ,I have few doubts regarding the video
1. Whether the fat is collected in the round bottom flask, or certain portions are in the extractor too, if I have to vacuum evaporate it ,do I need to mix the both the portions, ( the Solvent in the RB as well as in the extractor)
2. What's the quantity of solvent taken for 250 ml RB ( as shown in the video), can I perform the same analysis if I have 500 ml Soxhlet extractor ,in that case what would be the amount of solvent.
Please clear this doubts,
@@SourodiptoOfficial Fat is only collected in the flask. So you have to dry & consider the flask only.
Secondly, there is no specific amount of solvent to be added. Amount of solvent should be enough to carry out the continuous reflux through the Soxhlet. You can get idea of amount of solvent from this video. So add solvent in your 500ml Soxhlet accordingly which is enough to recycle/reflux smoothly.
Could I use diethyl ether instead of n- hexane?
Yes. You can use
good video
please could you define the principle of this test? its impotent........
Vinit Sharma, thanks for your observations. I planned to explain the principle of each of the tests in separate video. Please be with use. We will work on it very soon.
I have used your method but my results when m calculating I got 20.4 instead of 2.04 following the Crude fat content for Crushing Soya beans please help me with calculation
The calculation given in this video is correct. So please calculate your result following this video.
I think that the weight of sample=weight of sample+ thimble - weight of thimble=4.7152-1.9177=2.7975 Right?
No Sir. Sample weight should be 4.7152. Because we tared (made zero) of the thimble weight on the banace machine before taking the sample in it. So, the thimble weight will not be considered.
@@MicroChemsExperiments Thank you for the clear explanation!
@@tranthanhhoang2726You are welcome
Nice background song ⚡🤗
Thanks
Sir plz share video of determination of ash and moisture from milk
th-cam.com/video/n2Qwb8Pw8YE/w-d-xo.html
th-cam.com/video/DtSH8n1jbyM/w-d-xo.html
Can we extract coconut oil by using this method?
You can
Sir plz make a video on determination of carbohydrates in food
We have already made.
Video link: th-cam.com/video/kCzY0arQkN8/w-d-xo.html
@@MicroChemsExperiments ok thank u sir
@@maahii8335 You are most welcome
Please make video for RM value of oil/fat
Noted the test perameter. We will start working on it. Be with us.
We can petroleum ether instead of hexane
Yes. You can use
Why only n hexane is used for that , and not any other solvant
You can also use Petroleum Ether
How to found percentage of oil in between rice bran?
Same method should be followed
what is the size of thimble used here?
you can use any size of thimble which fit into the condenser of Soxhlet.
Can we use liquid sample like milk?
Thanh Nguyen, No, you can't determine the fat/oil content of a liquid sample by this method. This method is valid only for the solid sample. But you can determine the oil/fat content of Milk sample following our video, link is given below...
Video link: th-cam.com/video/muswKsqvGnM/w-d-xo.html
Can I determine fat content of milk powder by this method???
Yes
@@MicroChemsExperiments Tnx
Temp??
What was the amount of n hexane u added
Not specific amount. Add enough amount to reflux
Please sir, I would very helpful if you give me some details of Soxhlet Apparatus
Take help from google...
suprrb
Thank you
👌👌👌❤️
Thanks 😊
@@MicroChemsExperiments I'm using right now ethanol and water as solvent. Can I do with light weight oil and ethanol as solvent for extraction..I want oil soluble extracts or extraction in oil. If so which oil would be best. Kindly suggest. I'm mostly into native siddha ayurveda formulations.
@@MicroChemsExperiments we generally soak the herbs for 12hrs in water alcohol mixture. And then next day make extract in soxhlet extractor. And the extraction is incorporated into base oils as per body type and heated on low temp until moisture evaporate. And the result is medicated oil. Where each herb has different weights.
Before incorporating herbal liquid extract into oil , oil is heated separately until one froth. Then only we add liquid herbal extract. This step is very important. So I was thinking can I add oil and alcohol as solvent n do extraction in soxhlet
@@theasoapsacademy Thank you for your query. If you want to go for oil extraction, you can use Organic Argon Oil (Light Weight Oil), if it does not distort your herbs or extracts. In this case, you have to extract using oil and ethanol separately because oil will not dissolve with ethanol and they both have different boiling point.
after that how can purify oil?
Hexane residue can be removed by keeping and drying it into hot air oven at 130 degree Celsius
How 2determine if it is not solid sample...how 2 determine fat of raw carrot sir??
1. First of all you need to dry your raw sample.
2. Then ground the sample homogeneously.
3. Test the sample for fat determination following this video
Sir is itnot possible without drying it??i want the value of raw for my researchworks
@@mayakoirala33 You can try raw sample. In that case take about 15g raw blended sample for testing.
Sir plz explaine how to analysis in liquid
Different method of extraction is needed. We will develop that method later.
hi sir. may I know, can I used this analysis for a food paste product (semi-liquid)?
Hello Ma'am,
May be you can.
Try it in your laboratory.
But It was recommended this method for dry powdered sample.
What about the liquid sample..plz reply sir
This method is not applicable for liquid sample. If your sample is milk or milk product then follow this video: th-cam.com/video/muswKsqvGnM/w-d-xo.html.
But if the sample is other than milk then we will develop video for it later. Stay with us.
@@MicroChemsExperiments Have you made it ?
Is the extracted fat is in oleic acid?
No. It's total fat
@@MicroChemsExperiments it's edible right?
@@ronelbanci4599 Yes. Edible oil
@@MicroChemsExperiments do you have knowledge about oleic acid? because my professor keep on asking me how i could confirm the extracted oil is in oleic acid when using Soxhlet Method.
@@ronelbanci4599 Oleic acid is a major component of oil/fat but you can not extract only oleic acid from sample seperately using soxhlet extraction method.
But you can quantify oleic acid from sample by applying two seperate steps (methods):
1. First step (1st method): Extract total fat by following this video of soxhlet extraction. After the soxhlet extraction, you will get total oil at the bottom of the flask.
2. Second step (2nd method): Take 0.2g of extracted oil (from soxhlet extracted oil) to saponify and to make the Fatty Acid Methyl Esters. Then analyze the Fatty Acid Methyl Esters in Gas Chromatography (GC) and quantify the Oleic acid from the Chromatogram of Fatty Acid Profile of that oil.
***I hope you have got my point***
What was taken in rb
rb means??
3.21 cfu/g is my reading
.: Then how many colonies are there
There are about 3 colonies in 1g of your sample.
@@MicroChemsExperiments how can you calculate sir