How to Count Cells Using ImageJ | How to Count Cells in Imagej | Imagej Cell Counting |
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- เผยแพร่เมื่อ 29 พ.ค. 2024
- How to Count Cells Using ImageJ | How to Count Cells in Imagej | Counting Cells on Specific area | imagej cell counting
This lecture describes not only how to count total number of cells from positively stained immunohistochemistry / immunofluorescence images but also describes how to count positive cells on specific area of the image.
For Quantification of Immunohistochemistry images using ImageJ, please watch this video:
• Quantification of Immu...
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This video is for educational purposes only.Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, comment, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational or personal use tips the balance in favor of fair use.
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Image>Type>8bit
Image>Adjust>Threshold
Process>Binary>MakeBinary
Process>Binary>Convert to Mask
Process>Binary>Watershed
Analyze> Analyze Particles
Hi Bio Lectures, how would I add ICTN (Image-based Tool for Counting Nuclei) onto the windows imagej application?
Excellent description 😊
Glad you think so!
Very helpful video specially counting cells on specific area of the section if ihc image
Glad you liked it
nice and easy method. My next question is, how do I do the final calculations. I mean how to present this kind of analysis. Most of the papers about IHC, present there data as, cell count/mm2 or mm3. How to reach this type of results after following your method of call counts. Thanks in advance.
Very helpful 👍
Glad it was helpful!
thank you very much. It is very helpful. How to save the same shape , I mean the same measures of the area? I want to have the same measures for this area in all my pictures. Do have any tips?
You are welcome! If you want to maintain the same measurement scale in all your pictures in ImageJ, you can use the "Set Scale" function. This will allow you to define a known measurement for a known distance in the image, which ImageJ can then use to calculate the scale for the rest of the image.
Here are the steps to use the "Set Scale" function in ImageJ:
Open your image in ImageJ.
Use the "Measure" tool to measure a known distance in the image. For example, if you have a ruler in the image, measure the length of the ruler.
Go to the "Image" menu and select "Properties".
In the "Properties" window, click on the "Calibration" tab.
In the "Spatial Calibration" section, enter the known distance you measured in step 2 in the "Known distance" field. Enter the units of measurement in the "Unit of length" field.
Click on the "Global" button to apply the calibration to all images that are opened in ImageJ.
Click "OK" to save the calibration.
After setting the scale for your image, you can use the "Analyze" function to measure the area of your object. The measurement will be displayed in the units you specified in the "Set Scale" function and will be consistent across all your images
Excellent explanation 😁
Thank you ❤️
Can i count senescent cells with this method?
Yes, ImageJ can be used to count senescent cells.
For each counted cell I would like to determine if it is senescent or not, defining a sort of threshold.. how can I do it?