We're really glad that you enjoyed the webinar! We don't have current webinars planned to talk about other PPIs, but will definitely keep the request in mind :)
This is an interesting seminar. I have a question. In the case where I only have a protein X as the bait and I am interested in just any other proteins which protein X interacts with. How do I design this experiment with respect to the TAD?
Hi, just in case you haven't found the answer yet, you could create a Y2Y cDNA library system that could act as the prey. Details construction of the Y2Y cDNA library system could be found in this article (doi.org/10.5423/PPJ.OA.01.2015.0001 ). You may adapt this technique accordingly.
![[FULL EP.73] เปิดบ้าน 70 ล้าน "แป้ง Zbing" ยกมาทั้งญี่ปุ่น l เคหสถานบานปลาย l One Playground](http://i.ytimg.com/vi/YBnJhTt07Pg/mqdefault.jpg)
I am really impressed with the presentation!!! that's how a lecture should get delivered instead of a long boring presentation.
This was awesome, came looking for a quick answer but stayed for the whole lecture. Really well explained, thanks!
Such a good explanation! Thank you so much Alan and keep going! Greetings from Germany
I really appreciate how excited the presenter is. This was a great explanation of a complicated process.
Very simple and easy to understand. Thank you.
The start was from a very basic level. That gave a kick to go through the entire concept. Nice.. ☺☺☺
The lecture is very interactive even through it is remote, and the concepts are well explained. 🤩🤩
You teach very well, thx for the upload.
more clear that water,excellent !!!
awesome thank you! i needed the background
Wonderful webinar.. thank you.. can you explain other PPIs too...
We're really glad that you enjoyed the webinar! We don't have current webinars planned to talk about other PPIs, but will definitely keep the request in mind :)
ugh, the ONLY good PPI video I've EVER SEEN
Awesome webinar ❤❤❤
Couldn’t we use a phosphatase treatment to answer the question in 33:46?
how can we bind protein with the nucleotide sequence of the transcription factor ?
This is an interesting seminar. I have a question. In the case where I only have a protein X as the bait and I am interested in just any other proteins which protein X interacts with. How do I design this experiment with respect to the TAD?
Hi, just in case you haven't found the answer yet, you could create a Y2Y cDNA library system that could act as the prey. Details construction of the Y2Y cDNA library system could be found in this article (doi.org/10.5423/PPJ.OA.01.2015.0001
). You may adapt this technique accordingly.
Shout outs my bro Alan
what kind of detection system is used to find positive hits?
What is the false positive in this assay