UV effects on bacteria time-lapse
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- เผยแพร่เมื่อ 30 ม.ค. 2017
- Time-lapse footage of bacteria-covered petri plates growing after being exposed to UV light. UV light kills bacteria, with longer exposure times resulting in higher cell mortality.
The elapsed time is shown in each clip. The petri plates were incubated at about 37 degrees Celsius.
The lamp used has two 15 watt bulbs. The lamp has 110 volts and the wavelength is 254 nm, UV-C. - วิทยาศาสตร์และเทคโนโลยี
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what if all the bacteria just ran away to the other side?
E. coli can't move on a solid medium. It would take them a very long time to move to the other side as well.
Sci- Inspi Not to mention that not all strains of E. coli are motile. Perhaps you could have used a nonmotile strain.
@@sci-inspi r/woosh
@@davidgamingremastered6784 r/notawooosh
@@davidgamingremastered6784 r/plzcommitapoptosis
What if bacteria apply sunscreen..???
It should protect them. If the chemicals in the sunscreen aren't bad for them in the first place.
If the sunscreen is zinc based, they should be fine. If the sunscreen is carbon based, the UV will just heat it up and they’ll either incubate or die trying.
Absolutely brilliant 👏👏👏👏👏👏
Let’s just hope no bacteria’s read this comment or else we’ll have a real problem on our hands.
@@sci-inspi I love how you respond to these questions that were meant to be a joke. Really shows how there are no stupid questions.
'Bacteria' is already plural
Germs: 'exist'
Light: im gonna end this man's whole career
The sun is a deadly laser
Did he just write them on his death note or something?
Low Quality Shitposts not anymore there's a blanket
A bacteria isn't a humanoid, if you are gonna meme do it properly. T_T
You should spread the colonies who survived into a new petri dish and repeat the process until they all survive.
That's a good way to demonstrate adaption and evolution! Thank you.
Lol
Yep
That's a good way to create a damn superbug, don't do it!
To create a new super bacteria. No, don't do that.
Why is there so many people talkin 'bout gloves? I work in a microbiology lab on pharma and we do not wear gloves. It' s not like splashes or something. It's all about knowing how to handle them. I believe you did it just fine. Normaly people who's afraid without knolwedge of bacterias get scared.
Larissa Amores umm! maybe because...its E-coli.
@@whendeathdeclareswar7458 Do you know anything about E. coli besides "It makes you very sick"?
You are a part of the problem Larissa describes.
@@whendeathdeclareswar7458 how you shit
if he gets e coli on his hand he could just wash it off
Yeah, in school, even when I worked with piranha solution or aqua regia I didn't really wear gloves. The only time we did was for hydrogen fluoride. If I didn't need to wear gloves for aqua regia you probably don't need to wear gloves for E. coli, especially when there's already a shit ton on your skin.
love how he answer most of the comments
I try my best
When Thanos even wipes out half of bacteria
Wayy underrated
noes noes PÉGATE UN TIRO
_"To show you the power of UV light, I covered this specimen in half!"_
Protect yourself against the might of UV light with Flex Seal!
that is a laboratory UV source, more powerful than the battery UV-C wands.
@@mango251 that funny, you know Flexseal really liquid Rubber. UV light eats and destroys elastic polymer Rubber. Rubber enemy is UV light. Don't care if it's vulcanizing like tires they degrade. That why sunscreen like a Rubber protection cream lotion. You are actually protecting the dermis, elastin elastic rubbers band's fibers in our skin, from degradation and wrinkles. Our skin, flesh matter or whatever you call it is similar to a Rubber coverage, over our body's. The pigment melanin a natural sunscreen is natural built-in our DNA. But what most don't understand is, the more skin pigmentation we have the more the skin is tighter lest ability to stretch, the less pigmentation and dna of lighter skin types the more elastin rubber like the skin is. That the way DNA ,dermis etc is , but UV will destroy Flexseal🤣😃😄😄, and Rubber and skin.
Would be interesting to see the comparison between this video and other types of UV light being used
Hold up bacteria! it is i THANOS
SkiLLsZ u beat me to it,
Your profile picture make it better
upvote
The experiment is cool enough, but you provide explaination too? Perfect.
I was hoping a video like this existed. Thank you!
Thanks the video is a boon to explain community the effect of time and UV radiation on harmful ecoli found in so many water and food items we consume daily.
love these scientific demonstrations!
Excellent video! I was going to make a video showing UVC light tests using salmonella, but no need to now.
I honestly love watching bacteria colonies grow, it’s my favorite thing to watch, it’s interesting to see this though. It reminds me of my science fair project, how much bacteria does make up contain? I had a lot of fun watching the progress of the bacteria colonies and seeing them grow, it was very fun. I would like to try this one day at my schools lab, maybe with something different.
How did you quantify the amount of bacteria left over?
Like is there a way to compare the growth of the control side and experimental side?
Not sure if that makes sense, but thanks
Hi Katie,
Usually you can count the number of bacterial colonies and compare the different sides. Highly concentrated cultures often leave a "lawn" of bacteria which we can see in the control side. Hope that helps.
ImageJ is also typically used so you can measure the area of patches or lawns of bacteria
You can literally see the living bacteria with the naked eye. Couldn't you see the difference between the sides? And he was just counting how many separate groups (colonies) were remaining. Just the number of colonies and total size (area, because they're on a very flat surface) should be all we care about.... not the actual number of bacterium left over.
I don't think the OP took basic biology class >.>
*Casually pulls out E. Coli*
What if after being exposed the the UV light, you look down is shock as the E. coli takes of a pair of shades and says “Hey! Thanks for this nice, golden tan!”
Wait so that's not normal?
@U hahahahahahahahahhah
i saw u on the Lamborghini video too
Thank you for the explanation at the end
This is brilliant. Thank you.
The apocalypse: bacteria edition
Today this year it seems more like virus, but you were close my friend!
Afraid you have E. Coli on your skin!? No worries! Lay under a UV lamp for a few minutes. You'll be fine.
Thank you so much for the information!
Great video !! You are the best!!
Nice! E.coli! I like to spread that on my cheese peenut butter sammich
And how long you are eating shit?
@@4ipon4ik lmfao
*you are scaring the bacteria! Shush!*
Are you sure that it's peanut butter?
@@4ipon4ik that's a r/whooosh
Coronavirus:uhhhh why i can't infected you!!!?!
Me with full uv armor: i came prepare
Learnt a lot!
Keep posting such educational videos
स्टूडेंट की दुआ लगेगी😁
Very relevant these days! Thanks!
Wow, thanks for this! I will quit with overnight uv exposure. It was melting plastic tubes and Pipet tips
What distance was the lamp from the petri dish? Nice experiment, thank you!!
This guy answer everyone questions even silly one am subscribing
This is an awsome channel
Person: *AcHoO*
*BEGONE UNHOLY SPIRITS, I HAVE GOD AND UV ON MY SIDE*
E.coli: *Exsist*
Creator:* THE SUN IS A DEADLY LASER*
U r criminally underrated bro
Simple and nice. Thank you.
Who is here after COVID-19 ouybreak
I am! Remember to wash hands 👍
I am! I am looking to build a box to decontaminate packages before they go into the house without destroying it
SURVIVAL OUTPOST CHANNEL how do you figure? UVC rays do not pass through the ozone layer.
Seriously, can this kill viruses like covid-19 ??.
John Olson it does work, but it also kills everything else.
Using UV light to treat Covid in a person makes about as much sense as boiling them to sterilize them.
Great experiment! Will it get the same results if use UVB or UVA?
Very godd footage and good presentation. Subscribed!
Great Video!
Nice video, thanks for sharing. Would moving the light closer to the petri dish kill the bacteria faster? Just wondering...
I am not sure, but I think it would. I think bringing it closer would make it more intense I assume.
@@sci-inspi I'm not a biologist so idk if increasing light intensity would decrease disinfection time. But, I do know that uv follows the inverse square law so the light should be far more intense the closer it is.
@@Dysfunctionality15 I think it's also depend on UV wavelength, from all I know, 260-270 nm is the best to kill E.Coli. The lower is, the less effective. I'm not sure what happend if UV wavelength is higher.
I have thought of an experiment involving this method. I'll cover a plastic wrap above the petri dish after inoculation where I can apply a handful of sunscreen. In theory, this should filter, or at least some, UV rays; thus having more growth compared to those completely exposed to UV. It would be nice if you have an insight to this matter.
Plastic wrap would block the UV rays. An alternative would be a agar or clear overlay with sunscreen and see if that works.
This is also why every probiotic drink is in a black bottle, so that UV light doesn't kill the bacteria.
nail techs worldwide united with their uv lamps
Basically of reasons planets become deserts when the atmosphere is removed, constant UV bombardment.
So bacteria are mini vampires actually, themore you know.
THANK YOU!
the video is 3 years old, but I really liked it, I just missed seeing the PPE protection in your hands when handling the bacteria, or with your eyes in the ultraviolet light. I'm from Brazil and this video will help me do some research
so this is is the cure to pimples - UV. thank you for this.
That wouldn't be a good idea. UV light causes sunburn. And prolonged exposure of UV after years can cause skin cancer.
UV - C are lethal
It's great for psoriasis though because it kills all the cells that are rapidly regenerating.
@@sci-inspi if im correct, it also does give a thing called arc eye or welders eye or something? witch tends to be quite painfull.
Nice work. Can you also run that using O3 generated from 185 nm and no direct UV?
This is what I was also wondering. Ozone is heavier than air so it will fall down onto the uncovered side of the petri dish. Perhaps they could seal the dishes and remove all oxygen.
very interesting video we can learn a lot from this!
have u tried picking up the ones that grew after exposure, spread them, and re-expose? luv ur channel, btw!
Anyone else freaking out about him not using gloves while handling the E. coli? Lmao. My microbiology teacher always made sure we handled our Petri dishes with gloves.
FightingMario54321 nope
I know this may sound a bit insane, but if only we could do this same experiment but with even stronger EM waves to test how long microbes could withstand, like say, X-Ray.
A bunch of them would pass through, UV is pretty readily absorbed due to their respective photon energy levels.
Good video. Thanks
Very Nice!
What was the wavelength of your UV light? Did you use uva or uvb or uvc?
UVC is the highest energy and therefore more likely to form thymine dimers, making it most lethal.
How much E.Coli would i need for this for this experiment
one 5-milliliter 24 hour old broth culture of E. coli should be plenty.
Thank you
This was a very helpful video. Much appreciated. I'm sorry you had so many childish replies.
Your content is amazing! Can you do the same experiment with fungi instead of bacteria????
I'll add it to my list of future videos.
@@sci-inspi Thanks
can anyone tell me what is the best uv wand to get i want to make sure it's not a knock off and it kills the bacteria and i want one i can change the bulb if possible for toilet bed sheets and kitchen counter and my lazyboy. how would a person go about testing if it kills germs as it should.?
Jeannette K i believe any regular uv light will work but you can always buy some off of amazon good luck:)
it s not good to use UV light to kill bacteria in closed area like your toilet, because Uv light reacts with Oxigen O2 in the air to form ozone O3, which is toxic!
i recieved a light in the mail with no instruction it is led and it is blue it showed how what surfices needed to be cleaned I think it is a black light. Is there such thing as a led uvc light?
+Hung Tr Ozone has no smell, this thing we are feeling is nitrous oxide and this is real threat. Because ozone is hyperactive then easy oxidate nitrogen.
Those with the strongest immune systems grew up playing in the dirt, those with the weakest immune systems grew up wrapped in cotton wool.
Nice explained
Thank you
Thanks for the video, very cool. How close were the 15w bulbs to the dish? Thanks
Can you do a video on the effects of alcohol on microbes? It's kinda relevant nowadays. Thanks!
(I liked and suscribed) so if I leave something out in the sun for 2 minutes, will all the bacteria on it die?
Not all the bacteria, but some will die.
These lights are UV-C which cannot penetrate or atmosphere.
Really love your music :)
Thank you!
Isn't it weird how he handles bacteria so normally? No gloves, no equipment, just swab like there's no tomorrow.
hii,
you have got a good video.
would you do a video on Ozon air toward bacteria and mold pls ? there are lot of cheap ceramic ozone generator on ebay..
thanks
andrew
Let me check and see if I can get my hands on some Ozone.
great!! can't wait.. thanks
The real question is, where on earth did you GET E COLI??
I bought it from scientific suppliers.
its ecoli not ebola lmao
So basically bacterias are just microscopic vampires
Nah everything else is just a bigger and tougher vampire that can handle more UV.
That face when he accidentally makes a super bug that can survive UV
doubt it. Since it's destroyed at the DNA level
Is the light here uv a, uv b, or uv c light
UVC
thanks
Do the same experiment with various levels of alcohol and hydrogen peroxide.
And bleach. That should be interesting.
I love this! !
Cool guitar towards the end.
So to protect myself from bacteria I just have to stand under an extremely intense UV light source for extended periods of time. Maybe if UV kills bacteria, it can also kill skin cancer. I think I just earned myself a noble prize.
@Dušan Xmetov And that's exactly how radiotherapy works!
Except it uses x-rays instead of UV radiation. (The former is more energetic)
UV kills every cell, high UV exposition is bad for your body.
Garlic Bread that’s kinda the whole joke
@@Sir_Budginton Yeah, since UV is literally the cause of skin cancer. Don't know how garlic didn't get it. :l
is one minute too much for the skin?-how much is too much exposure?
Does UV light have any effect on viruses, and if so, how much exposure is needed to kill them, especially hard-to-kill viruses like SARS-CoV2?
Cov-sars-2 isn't "hard to kill", just use soap or any disinfectant. The problem is, it *spreads* really easily.
@@NigelTolley Billetes, mascarillas, verduras talvez
I love how he just handles potentially dangerous bacteria with no gloves on
Eh, the bacteria used in a lot of labs are non pathogenic, and if you use proper sterilization, handling, and clean up techniques it’s fine. You can use gloves for extra protection though (I generally do because I’m clumsy sometimes)
ive literally ate E.Coli its fine
a highly educating video clip with interesting comments
*Grabs E. Coli with his bare hands*
No he grabbed a test tube. The E. coli is inside the test tube
What temperature did you incubate the bacteria in??
37 degrees Celsius
Thank you. I was researching this on science buddies and they said 37 degrees Celsius but I wasn't sure and so I asked you what you did.
You're welcome Harish.
The bulbs are LP or MP?How distant are lamps from the plate? have you irradiated the plates in a room or outdoors?
Thanks!
Madlad not wearing gloves when handling E. Coli
Would a simple 3W UV LED be enough ?
I am not sure. I used a lamp that had two 15 watt bulbs for this video. My guess would be the 3 Watt lamp would require more time to kill the bacteria. If you have access to a microbiology lab, you may be able to test the 3 watt lamp it.
what if you got a 5 gal of bucket of yeast and water that would take forever?
Sci- Inspi which wavlength in nanometers was the lamp you used?
The wavelength was 254 nanometers
Yes the wavelength is 254 nanometers. Sorry for the late reply, I never got a notification for this comment.
What was the separation of the UV tube to the plate , and by 15W I assume you mean 15W of energy going in to the tubes and x% efficiency?
Great Video! Whic doses have you applied? 40mJ/cm2 for 30sec? and for 2 minutes? Thanks!
Hi EOS,
I don't know the dose but I could give you information about the conditions and maybe we can calculate it?
2:45
High level stuff
That's quite fast
6 likes only lmao
Now I’m happier knowing my local chicken restaurant has a UV lamp prominently hanging up on the kitchen wall.
rad little riffs
Whatever questions emerged, I'll answer them all as an educator. Thanks, Sci- Inspi
I try my best
Now you know that summer time is the worst time for cold and flu viruses.
Amazing
Have u ever been sick from the work u handle with ur hands. I'm cringing so hard my face is gonna get stuck like this lol. But I'm here for this
Nope, never.
I love the music in this video
They've been using that system for years in the water purification plants
Is there particular brand of UV lamp?
What are the specs?
Been thinking of using UV light to kill mold on peanuts.
Also, is there a national or international organization with information on UV lighting and its uses?
Thanks
distance to the light may also play an important factor in addition to the length of exposure.