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- เผยแพร่เมื่อ 22 ก.ย. 2024
- In this video i teardown a $24,000 Axon Instruments GenePix 4000B Microarray Scanner manufactured in 2001.
Microarray scanners are used in gene and DNA analysis and are able to scan arrays in very high resolution using lasers to make markers emit fluorescent light.
en.wikipedia.o...
dexterslab2013....
/ dexterslab2013
Really enjoyed your video. Great stuff.
I'm not a native speaker (still learning) and that's why I am very thankful about your clear pronunciation.
I always have two metric (Inbus) and two imperial (allen key) sets next to my bench.
The DAC is probably for the linear slider driver.
Subscribed to your channel. Happy to see more teardown in future.
+Max Koschuh Thank you for your kind words. I hope in the future i can provide CC closed-captions, subtitles etc which means you can watch with your native language as subtitles.
FYI - Axon in micro-array scanning is like the Coherent for lasers, Rohde and Schwarz for test gear, Mitutoyo for calipers, or Ingersoll Rand for industrial air tools. They still are the kings in modern sequencing (though this gear is the equivalent of a 386dx). Those linear actuators/controllers probably have .5 mic tolerance and absolutely no play. Ideal for precision machining on a lathe for example (you could bore/hone an engine to Formula 1 precision with that tooling).
edit: This works with GenePix Pro software on stock computers (generally dedicated computers don't come bundled with scientific machinery until you reach the 100k mark). You can download it off the manufacturers website. Some of those optics you identified as mirrors were notch filters or glan polarizers. (edit 2: mike beat me to it). Also holy shit 30GBP? If you can fix it up in its entirety you could probably get 3 or 4k for it, uni's generally love these older generation gear. Get in contact with me if you want to sell some of those parts off, especially the linear actuators, steppers, controllers, et al.
+wither8 Thanks for the additional info!
Having to wait for the end of a 9 hours long work day before being able to watch this video was extremely painfull.
+msylvain59 they say good things come to those who wait!
If it's using fluorescence, the filters will probably be to block the excitation laser wavelength and pass the flourescence wavelength. Look like very narrowband filters.
Slanted window on red may be to select prolarisation.
Change in green laser current will be peltiers stabilising diode and maybe also cavity/ktp temperature.
Would be interesting to take a closer look at that linear sensor on the voice coil.
I do wonder if they could have made it a lot simpler & cheaper by adapting CD player type mechanics & optics. But I suppose people paying $24K expect a decent amount of weight for their money....
+mikeselectricstuff Thanks mike, yes your right about the filters, i had a play after i stopped filming, they do seem to attenuate the laser light and the light from the florescent marker is slightly different frequency from the laser.
I'm not sure how dynamic the focus would have been, it's a shame i never saw it working but i dont think would need to be as fast as a CD focus, i wonder if it was just to allow a 'per-scan' recalibration for differences in thickness of the slide it was processing.
Might take a look at the sensor, i will be keeping the main x-y table bits for the moment.
[offtopic: Many years ago I've torn down a magnetooptical drive using voicecoil positioning instead of the common gear drive too, I was rather surprised, it was full of fancy optics, must have been a rather early drive.]
Fast power modulation of green frequency doubled lasers is hard, for optimum performance the laser and the KTP crystal both have to be at their optimal temperature, this operational point changes pith output power, so you wouldn't like to modulate output power by changing pump diode current in a precision instrument.
Beautiful piece of technology and great teardown!
You could likely still re-purpose the main tray as a high-resolution scanner. Incidentally, I had just been pondering making a similar mechanical arrangement for high-res scanning of film negatives -- looks like I'll need to keep my eye on the surplus market for microarray scanners! The voice coil drive on one axis is especially nice. PMTs are not too difficult to deal with, and I bet the analog electronics and power supplies for them on this thing still just work. Similarly, steppers are pretty easy to drive. The nice thing about device in this volume and price range is that they do use off the shelf modules, like the HV power supplies and laser drivers, instead of just one highly integrated and inscrutable board.
+Jonny Hall If the main board had not have been faulty i would have thought about doing this, i have some microfiches that would be nice to see how they looked scanned. But its been stripped now, the linear stage is on ebay, hopefully it can pay towards future teardowns!
That sensor is a non-absolute optical glass encoder.
They cost an absolute fortune, especially this accurate. I'd keep it in one piece and figure out how to drive it - that could make a very useful fast actuator.
The larger stage(Y?) with the stepper is also very expensive - they go in thousands, and the micron-accurate ones go into tens of thousands of dollars.
Those PMTs are tiny, have an insane amount of stages for their size, and have an amazing spectral response - keep them, preferably in a velvet gold-plated box.
Take that lens out of the tube(two tabs on the side, use a credit card or a comically oversized coin) - use it for macro on your camera! :)
I'd also try using the Y stage with a camera to create stitched gigapixel macro images. It is rather long throw, and you can have it move relatively easily with a simple stepper driver. Stick a specimen on top, put a camera somewhere stable(same plate + some bodgery?), and figure out how many steps it takes to move it out of the FOV.
+Spirit Thanks, some interesting ideas there, the mechanical bits will be going up on my 'i dont have a use for this but i could not possibly scrap it' shelf!!
+Spirit I think i tracked the linear stage down to this, but no idea of it's cost: dovermotion.com/product-catalog/linear-stages/micro-linear-stage-kv/
DextersLab2013 From what I can find it costs around $1500.
Man I find your comments on all of the cool TH-cam videos pertaining to lasers, optics, and spectroscopy stuff. I’ve checked your channel… And don’t really see that type of content in your videos. But you seem extremely knowledgeable and I would love to see this type of content from you !
@@hullinstruments I'm working on it. Stay tuned, possibly indefinitely :)
New England Affiliated Technology...NEAT!
You use the metric system & you say AluminIum. wow, i instantly subscribed!
i wouldnt know what any of that shit was but id love taking it appart
Talking with your hands is freaking me out. lol
Cool thanks
The black on the inside is probably RF shielding paint.
More to suppress photonic activity.
Niiice
can i hath the wiuers please
can i hath the lazes please
Does anyone knows anything about the drop logo on the housing ? It is now the one from Cytiva ( ge health care life science spin-out ) but I am extremely surprised to see that it existed before !!!
Hey do you still have the actuators from this I have something like it and both actuators have had significant damage to them I am willing to pay for them thanks :)
By the way I'm not Aussie50 haha
+Aussie50 The NEAT linear stage with the stepper was sold on ebay a few weeks ago i'm afraid but the other linear arm with the large voicecoil i do still have. You can get my email address through my channel about page if you want to contact me.
+DextersLab2013 hey I don't know how to get your email sorry please can you email me my address is mrdomdom3@gmail.com :) thanks
+Dominic Scott If you go here: th-cam.com/users/DextersLab2013about there is an option to view email for business enquiries
HOUSE number 14
I have one of these units, the tray is stuck and the damper won't turn. Was going to bypass the cables going to the stepper motor, but I would be helpful knowing the model # of the stepper motor. However I am sure 8 years later your unit is gone, Just asking incase someone else has such a unit.
I haven't seen one of those shitty Microsoft Optical mice in a long time.
That's cheap
Want to sell it ? Send message. Tim
Sorry, it was stripped to make the video, anything salvageable was sold on ebay. I don't have anything left.