Luciferase reporter assay | What is luciferase assay used for? | Applications of Luciferase assay

แชร์
ฝัง
  • เผยแพร่เมื่อ 20 พ.ย. 2024

ความคิดเห็น • 79

  • @jonashoffmann8225
    @jonashoffmann8225 2 ปีที่แล้ว +8

    The best Video I've found. Thanks man❤️👍

  • @williamcheng8813
    @williamcheng8813 16 ชั่วโมงที่ผ่านมา +1

    the GOAT appears when we need him most

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  16 ชั่วโมงที่ผ่านมา

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  16 ชั่วโมงที่ผ่านมา

      check out our biotechniques playlist for all technical advances in biology

  • @JaceTan-90
    @JaceTan-90 4 หลายเดือนก่อน +1

    Clear, visually simple to digest and easy to recall! Many thanks.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 หลายเดือนก่อน

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @jaimayy
    @jaimayy 7 หลายเดือนก่อน +1

    I'm writing an essay on luciferins in biomedical research, and this video has been incredibly useful! thank you so much!!!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 หลายเดือนก่อน +1

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 หลายเดือนก่อน +1

      don't forget to subscribe

    • @jaimayy
      @jaimayy 7 หลายเดือนก่อน

      @@animatedbiologywitharpan of course!

  • @percypercy4810
    @percypercy4810 ปีที่แล้ว +4

    Thank you so much. This video was extremely helpful

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว

      Please support my channel via super thanks. Your small contribution like 1$ means a lot for us. The super thanks option is present at the lower right corner of any video, a heart sign with $ in it.

  • @maziastro9932
    @maziastro9932 2 หลายเดือนก่อน +2

    5:47
    How would we know whether the promotor is NOT BEING EXPRESSED AT ALL or is a REPRESSOR?

  • @muditgupta5968
    @muditgupta5968 2 ปีที่แล้ว +4

    Hi, Thanks Arpan, wonderfully explained!!!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 ปีที่แล้ว

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Small contribution like Rs50/100 means a lot for us.

  • @mamacocolilo105
    @mamacocolilo105 2 ปีที่แล้ว +11

    So much about this bothers me. Starting with the first 7 letters of this chemical's name. Good presentation though. Thank u

    • @shawnmulberry774
      @shawnmulberry774 2 ปีที่แล้ว +8

      It is from the Latin and it means "light bearing". Luciferin is the base protein. The last three letters (-ase) have meaning as well - an enzyme. I was able to synthesize this in my organic chemistry class and it is quite fascinating. It also did not cause anyone any harm. Turn the lights down and you can see the reaction. *Very* useful tool in biology. No need for fear or superstition - it is simply a name that describes what it does. Luciferin is in fireflies - lol and many other things. Sometimes you can see glowing waves of dinoflagellates in tocean waves at night.

    • @mamacocolilo105
      @mamacocolilo105 2 ปีที่แล้ว +3

      @@shawnmulberry774 thank u for that! Yes, I was aware of that. I have a degree in a health related field and we touched upon it when i took chem. As beautiful as it is (i believe its also in bioluminescent algae), as a Christian, the name just rubs me the wrong way. Just like the telescope they have in the Vatican. 😉

    • @shawnmulberry774
      @shawnmulberry774 2 ปีที่แล้ว +2

      @@mamacocolilo105 If im not mistaken, that particular name does not show up until the King James version. Or at least not in any of the "original" versions of Gospel which would have been circulating before 300AD. Certainly isnt in the original Isaiah. It is really more of a nickname but I do understand your sentiment. Also, the Large Binocular Telescope Near-infrared Spectroscopic Utility with Camera and Integral Field Unit for Extragalactic Research requires a lot of creative choices to get the acronymn LUCIFER. Why isn't it LBTNISUCIFUER? Real astronomers just call it the LBT. This reminds me of NON-scientists calling the Higgs Boson "the God particle". It may be important but come on...

    • @mamacocolilo105
      @mamacocolilo105 2 ปีที่แล้ว +1

      @@shawnmulberry774 Agreed. I always thought that science was supposed to be secular. Maybe if the name of the element that was discovered didn't carry such negatively charged name.
      Like the number 666 what will people think of usually? But people don't know that this used to be code for Nero Caesar (the most evil man of the time). Although its just a number, I've known people who have refused license plates if the numbers are in sequence, or people who refuse to pay a bill (they will ask for a dollar discount or to add another dollar to the amount). Its crazy, but I understand
      We understandably so take down statues/flags/change the name of places named in honor of historical figures whom other individuals may find offensive or might be sensitive to because of history; and yet when it comes to religion, its not the same (both good or bad, regardless). I wonder why.

    • @shawnmulberry774
      @shawnmulberry774 2 ปีที่แล้ว +2

      @@mamacocolilo105 Well, for a long time to be educated, you were probably in the "priest class". My degree is in genetics and probably the most notable founding father of genetics is Gregor Mendel and his famous pea gardening experiments. He was a friar. I think you can be a scientist who is not secular if you have the right attitude. This is not so easy. Even "The Origin of Species" by Charles Darwin is full of homage to how incredible God's creation is. He clearly looked at natural selection and evolution as the way God creates. Often people don't know this because they don't read his work, they read about his work.

  • @Анжелика-к9э
    @Анжелика-к9э 7 หลายเดือนก่อน +1

    thank you so much from Russia

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 หลายเดือนก่อน

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @sippakorn1410
    @sippakorn1410 2 หลายเดือนก่อน +1

    Thank you for your useful video

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 หลายเดือนก่อน

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @julia-ho9yg
    @julia-ho9yg 8 หลายเดือนก่อน

    omg this explanation was so good, thank you!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  8 หลายเดือนก่อน

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @bizhenyu
    @bizhenyu 8 หลายเดือนก่อน +1

    This explanation is really good, thank you!n

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  8 หลายเดือนก่อน

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @soundaryamohan6406
    @soundaryamohan6406 ปีที่แล้ว +2

    Excellent explanation

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

  • @juthibiswas94
    @juthibiswas94 ปีที่แล้ว +1

    Nice explanation. Could you please make videos on TF profiling?

  • @amruthalakshmi2762
    @amruthalakshmi2762 2 ปีที่แล้ว +3

    Nice video and explained very well

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 ปีที่แล้ว +1

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

    • @amruthalakshmi2762
      @amruthalakshmi2762 2 ปีที่แล้ว +2

      Yes sir sure

  • @星胡-r4q
    @星胡-r4q ปีที่แล้ว +1

    hello thanks for shareing this video to explain the assay!my question is where renilla internal control gene insert to the gene of interested?tyvm

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว +1

      Various plasmids encoding Renilla luciferase under different promoter constructs are commercially available. The validity of the use of Renilla luciferase as an internal control is based on the assumption that it is constitutively expressed in transfected cells and that its constitutive expression is not modulated by experimental factors that could result in either the upregulation or the downregulation of the amounts of the enzyme produced. During the past ten years, a number of reports have appeared that identified a variety of conditions that could alter the basal constitutive expression of Renilla luciferase.

  • @mitchell186199
    @mitchell186199 ปีที่แล้ว

    Excellent video!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว

      Please share my channel link with your friends and help me to reach big audiance namaste 🙏

  • @guihuajia7696
    @guihuajia7696 ปีที่แล้ว +3

    Hello, could you please let me know how to produce dsRNA?

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว

      You mean cDNA ?

    • @guihuajia7696
      @guihuajia7696 ปีที่แล้ว +1

      @@animatedbiologywitharpan Sorry, I don't know what was it exactly, but I refferred was "dsRNA" that makes target gene silence. Do you always produce cDNA to generate the so-callled dsRNA?

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว +2

      @@guihuajia7696 may be you are talking about RNAi

    • @guihuajia7696
      @guihuajia7696 ปีที่แล้ว

      @@animatedbiologywitharpan Yes sir, i were talking about it. Thank you.

  • @luismatheu4226
    @luismatheu4226 ปีที่แล้ว +2

    Great vídeo thanks

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว +1

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว +1

      Don't forget to checkout my playlist

  • @InnovativeSaint
    @InnovativeSaint 3 หลายเดือนก่อน +1

    I know Lucifer means light, and it would seem many advances in medicine have a Latin predisposition, nevertheless, Lucifer race (how it sounds) seems quite interesting.

  • @guihuajia7696
    @guihuajia7696 ปีที่แล้ว +1

    very useful!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว

      Please share my channel link with your friends and help me to reach big audiance. namaste 🙏

  • @DrJacob-z6l
    @DrJacob-z6l 8 หลายเดือนก่อน +2

    Why it’s in a vector ?
    Why they trying to mess with human genomes ?

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  8 หลายเดือนก่อน

      This has nothing to do with a human . It's to test biological principles

    • @Hazendal777
      @Hazendal777 6 หลายเดือนก่อน +2

      to makes us slaves of the high " elite " 😢

    • @DrJacob-z6l
      @DrJacob-z6l 2 หลายเดือนก่อน

      @@animatedbiologywitharpan I got the fact sheet of the ingredients of the C shot when it first introduced and I saw this substance in it ….

  • @haiyen0325
    @haiyen0325 6 หลายเดือนก่อน +1

    Thank you

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  6 หลายเดือนก่อน +1

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @haiyen0325
      @haiyen0325 6 หลายเดือนก่อน

      @@animatedbiologywitharpan sure

  • @njm41
    @njm41 2 ปีที่แล้ว +1

    Thank you sir.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 ปีที่แล้ว

      Really glad to know it was useful. For producing high quality videos my channel needs your support.You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

  • @starzwoo
    @starzwoo 2 ปีที่แล้ว +1

    thank you!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 ปีที่แล้ว

      Watch my video on brain facts official cahnnel and don't forget to like. link th-cam.com/video/gCSKpNdJkZo/w-d-xo.html

  • @KeithMakank3
    @KeithMakank3 4 หลายเดือนก่อน +1

    THe power of christ compelss you!!

  • @AnnieO100
    @AnnieO100 ปีที่แล้ว +3

    It’s in the jab.

  • @MsMarkopolo123
    @MsMarkopolo123 2 ปีที่แล้ว +2

    It literally says lucif erase

    • @MsMarkopolo123
      @MsMarkopolo123 2 ปีที่แล้ว

      What is lucif erasing

    • @aqua6613
      @aqua6613 ปีที่แล้ว +1

      @@MsMarkopolo123 people who serve Christ

    • @IamFreefromtheWokeLeft
      @IamFreefromtheWokeLeft 6 หลายเดือนก่อน +1

      @@MsMarkopolo123 DNA

  • @AmruMagdy
    @AmruMagdy 11 หลายเดือนก่อน

    images (2) ببب«О Боже, позволь нам достичь Рамадана, достижения, которое изменит нашу ситуацию к лучшему, очистит наши души и наши внутренности, достижения милосердия, прощения и свободы от ада».

    • @DrJacob-z6l
      @DrJacob-z6l 8 หลายเดือนก่อน +1

      Jesus is God