Sir plz tell 1)why blood urea nitrogen Is a best indicator in USA than urea? 2)which is the best mathod among 3 those you have mentioned in this video? 3)and what difference can happen in blood urea and serum urea value?;
1. Chemical formula of urea is ( NH2 )2 >C=O i.e. Molecular weight of urea is 60 while nitrogen in it is 28 . 60/28 = 2.14 , so the conversion is blood urea nitrogen × 2.14 = blood urea , a calculative value . All the automatic instruments used in laboratory measure BUN which is converted to the urea by multiplying 2.14. Plus other substances like NH3 can interfere with urea estimation so its better to give value in BUN. 2. Urease is best for the estimation of urea as its kinetic method. 3. Blood urea is the misnomer term which is used from the very beginning. Actually we are estimating urea in Serum/plasma. So no such difference between Blood urea and Serum urea. Hope it helps.
Blank (B) is used for caliberation Value will come lower than other S and T Colour is lighter Standard (S) - used for identification Dark in colour compared to B Test (T) - used for finding unknown conc. Among these three T is darkest in colour
Great work. But I'd like to point out that After decimal we don't say the figure, we say the digits. E.g. 2.14 is not two point fourteen. It is two point one four.
Sir plz tell
1)why blood urea nitrogen Is a best indicator in USA than urea?
2)which is the best mathod among 3 those you have mentioned in this video?
3)and what difference can happen in blood urea and serum urea value?;
1. Chemical formula of urea is ( NH2 )2 >C=O i.e. Molecular weight of urea is 60 while nitrogen in it is 28 . 60/28 = 2.14 , so the conversion is blood urea nitrogen × 2.14 = blood urea , a calculative value .
All the automatic instruments used in laboratory measure BUN which is converted to the urea by multiplying 2.14. Plus other substances like NH3 can interfere with urea estimation so its better to give value in BUN.
2. Urease is best for the estimation of urea as its kinetic method.
3. Blood urea is the misnomer term which is used from the very beginning. Actually we are estimating urea in Serum/plasma. So no such difference between Blood urea and Serum urea.
Hope it helps.
@@Biochemistrybasics thank u Sir
🙏😊
Thank you so much sir for uploading this🙏🏻🙏🏻Its so so helpful for last minute exam revisions and hats off to your explanations 😊🙏🏻
Glad you found it useful
Lucidly explained sir thank you
My pleasure dear
It is good video and learn more about serum Urea. and technical knowledge for MLT students...
😊🙏. Spread the word.
best video sir,all doubt solve ...seen the video ,lots of thank you🙏☺
🙏😊
Thank u sir thank you so much❤❤😊😊❤❤❤❤❤
My pleasure ❤️😊
Such a great video 👏 Thanks a lot
My pleasure dear
Thankyou very much sir
🙏😊
Great sir❤
@@PremaSiriwardana-te4tq thank you
Sir ur awesome ....💐❣️
You are super awesome 😊
Sit what is the importance of 'blank ' and 'standard' in quantitative analysis.
Blank (B) is used for caliberation
Value will come lower than other S and T
Colour is lighter
Standard (S) - used for identification
Dark in colour compared to B
Test (T) - used for finding unknown conc.
Among these three T is darkest in colour
Tq
Most welcome dear
Love to listen you bro... ♥️
🙏
Keep making such videos
Definitely
How I will get this standard and Test solution for My laboratory. Please help me
For preparing Urea Standard of 30 mg% dilute 30 mg of urea in 100 ml DW
Same way for 25 mg%
@@Biochemistrybasics Thanks a lot Sir
Plz video upload on estimation of blood glucose by folin Wu method
Okay
Good job
Thanks a lot
What about patient , control..etc...
Sir I am Anjali DMLT it's Azotemia is it's DMLT class or not
Normal urea level in serum and normal level of urea in urine . please say sir
Normal urea level in urine is 8-20 mg/dl while normal blood urea is 15-45 mg/dl
How to do calculation of same by graph mathod?
Is urea reagent ready-made solution available commercially? Or you have made it conventionally?
Ready made available
@@Biochemistrybasics Thank u very much...Sir.
Hello.My urea is 19...is that ok,?
Yes it's normal
How do we prepare standard for Urea test ?
2gm Benzoic acid +1000ml water
What is blank .???
Estimation of sgot and sgpt🙏🙏please
Yes. Will upload it. 🙏😊
Sir its a informative explanation
Thank you
can we get pdf of this experiment?
Thanks dear. Please spare a few minutes and do spread the word about the channel among your batchmates and friends
Wow
👍
Great work. But I'd like to point out that
After decimal we don't say the figure, we say the digits.
E.g. 2.14 is not two point fourteen. It is two point one four.
Yes. 🙏
@@Biochemistrybasics glad to help
Sir subtitle zaroor dala kare video me.🫡❤️
Okay