Hybridization (microarray) | Biomolecules | MCAT | Khan Academy

[@g2k25d94]

I can't express how much this made sense at such a crucial time. Thanks a bunch.

[@chandrashekharchauhan4120]

Just wow.... How do you guys at khan academy make such brilliant content?? Such complicated stuff explained with such clarity and made really simple, as if I was watching it happen, live in the lab.
Thanks and love from India

[@danielmillan6144]

Don't listen what the people say thanks man you make amazing videos

[@Misstigrine]

cDNA from the mRNA are used. In the wells, there are single strand DNA not RNA.

[@darrelltan7603]

thanks bro

[@shaki6500]

some of these videos are so wrong and confusing :( thank you i was like what happen to cDNA part of it

[@sabil1998]

right that makes more sense

[@rasheedolajiire5135]

Thanks a gazillion for the clarification!

[@brookecarlson9110]

what the heck is wrong with the audio? It's repeating itself, and then sometimes disappearing..

[@1992deval]

This video has made understanding so simple, and its 7 years old yet the best video

[@ruhidyusifov5106]

Thank you for helping by sharing you guys are brilliant.

[@juliedao6860]

Thanks a lot! Very thorough and clear

[@TheOnlyRero]

i love khan academy videos .. but some of them like this one has a very low audio i can barley hear it!

[@nu.wa.n]

+TheOnlyRero If you are using Windows, go to your speaker properties and make sure 'Loudness equalization' is ticked under the Enhancements tab.

[@britneymichelle4]

THANK YOU!! I can finally hear this!!

[@sylee9165]

@@nu.wa.n Thanks!!

[@lolacasielleslapeira8936]

i love you guys, this is just fantastic!

[@sciencenerd7639]

amazing!

[@EilafBadr--]

Thanks a lot .This is so helpful

[@JimRohn-u8c]

Thank You so much! God Bless!

[@DrFelix125]

His mic is bit on the quiet side, but I've heard worse. Good explanation, thx!

[@2legit2hit]

Cuts out at:
0:25
1:35

[@gustavojuantorena]

Thank you!

[@dreamerscastle]

Thank you very much !

[@FabriceTheArtist]

Great video. But, in my textbook it says that the wells are filled with DNA, not mRNA.

[@kaustek]

There are different kinds of microarrays -- gene microarrays (DNA) and gene-expression microarrays (mRNA).

[@ZAsym]

@@kaustek ahh makes sense

[@Nisia44]

omg it was sooo helpfull, thank you VERY much ;)

[@s.b.5896]

Thank you very much :)

[@merna2724]

THANK YOU SOOOOOOO MUCH

[@uroojaarif9943]

I have a confusion about the radio labeling (yellow dots & blue dots) how is it done ? How can one know whether one is a cancer cell or normal cell in the first place ?

[@cdorman11]

As to the second question, it's the same as knowing where to take the biopsy from. It's what's growing rapidly, and showing up as dense tissue in an X-ray.

[@Quolon]

The video skipped one step, that was, after fluorescence labelling, both the cDNAs are mixed together as a solution, and this solution is then poured on the chip which further starts hybridisation with the complementary bases...

[@zhansayabauyrzhanova2492]

here is my question: how to label with yellow and blue? could u explain the mechanism of it? thank you for considering!

[@Colderlarry1]

GRACIAS

[@ErinRaciell]

Jesus I’m barely awake for my biotech midterm and this guys enthusiasm it’s not helping...

[@ss-nu8xx]

Yeah he is not your keeper. His job isn’t to keep you awake. Wtf

[@Quolon]

That means the complementary sequences present in that well (which aren't labelled fluoroscently) are known to us right??

[@AnkushSharma-zv5hv]

good

[@sarahali1788]

I swear his voice is Armando hasudungan ! Biology and medicine you tuber?

[@jsantana331]

In the well, would their be complementary RNA, or ssDNA complementary to the probed cDNA ?

[@mikell.6064]

The video was very poorly explained. The well doesn't contain RNA. It contains ssDna that hybridizes with complementary DNA from sample. The sample is obtained from reverse transcribing the RNA from the cell to cDNA. This CDNa is used to hybridizes with the well.
So in short the answer to your question is ssDNA

[@atti1120]

so is each well complementary to only 1 gene each?

[@sukhpreetsingh5603]

plz get some sleep

[@عمارالاسمري-ت4ف]

Please try to raise your voice, Couldn't complete the list because of that!

[@arrankambo3665]

this video needs a far better mic. i can barely hear what he's saying

[@kamilab131]

does up regulation mean the same as duplication of a specific gene ?

[@sumitgupta7983]

Upregulation means amplification of gene

[@sundasrashid6026]

dude just go to sleep

[@isaackogan269]

its 2:24am lmfaoooo hlep me

[@Bar0n0s]

we need some good subs. good work though

[@Vencomycin]

Where is cDNA???

[@juzy1022]

😃😃

[@soumasoum2048]

I need explication in French

[@Asura6969]

non

[@rimaakter2116]

Low sound

[@sara-qz8zw]

Is there anyone here to help me? . My master asked us to find (the mRNA -DNA ybridization use for which microbes?) Pleaseeee help me

[@menaameer6848]

sorry but you didn't make me understand

[@mohammado.eshkalak3140]

This guy has no enthusiasm whatsoever to teach. He must make you wake up instead of making you sleep. Dont like it.

[@christiankorkis630]

Kinda sounds like this guy needs a nap like Bernie

[@rizaln8315]

boring

[@manlikanamkaew2220]

Thank you~

[@arpittyagi6404]

Thanks..helps a lot..

[@vuongyenxuan]

Thank you so much.