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Microscopy: Fourier Space (Bo Huang)

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  • เผยแพร่เมื่อ 8 ส.ค. 2024
  • Learn more: www.ibiology.org/talks/fourie...
    The Fourier transform is intimately associated with microscopy, since the alternating planes occurring in the microscope (focal plane - back-focal plane, etc.) are related to each other by a function very similar to the Fourier transform. This lectures explains the Fourier transform in terms understandable to non-mathematicians, and explains the relations with microscopy.

ความคิดเห็น • 91

  • @jackknight1899
    @jackknight1899 4 ปีที่แล้ว +46

    10/10, by far the most intuitive and simple Fourier transform explaination applied in image processing

  • @aatt3209
    @aatt3209 4 ปีที่แล้ว +4

    I cannot believe how amazing this lecturer is to connect the lens and fourier transform, I have learned so much!

  • @elenadoering3562
    @elenadoering3562 5 ปีที่แล้ว +6

    Seriously thank you so much for this. I've been trying to understand FT for days and now it finally clicked!

  • @MdNasirUddinLaskarBD
    @MdNasirUddinLaskarBD 7 ปีที่แล้ว +4

    Best lecture to understand the signal processing perspective and Fourier transform of image.

  • @majedkikhia8614
    @majedkikhia8614 5 ปีที่แล้ว +3

    WoW! This is the best explanation of FFT one can find online!
    Thanks a lot!

  • @AllanMedeiros
    @AllanMedeiros 5 ปีที่แล้ว +2

    Awesome! Very clear the relationship between focal plane image and Fourier representation! Thank you!

  • @nobdan123
    @nobdan123 3 ปีที่แล้ว +2

    This video and the one on Fourier transform by 3blue1brown made my understanding of FFT much clearer. The best explanation. Lots of love and keep up making such content videos!

  • @igorg4129
    @igorg4129 4 ปีที่แล้ว

    Colleague, You are great! Thank you so much, I've seen almost hundred of movies, tried to read, nothing helped. Thank you so much. You are really great teacher, please, keep teaching.

  • @Penguinurvideos808
    @Penguinurvideos808 5 ปีที่แล้ว +2

    Extremely useful for understanding the concept of cryo-EM. Thank you!

  • @CatastrophicNickName
    @CatastrophicNickName 4 ปีที่แล้ว +7

    Love it, I have been seeking a simple explanation to kickoff some intuition. Please keep on doing your amazing work :)

  • @Mojiube
    @Mojiube 7 ปีที่แล้ว +1

    Thank you very very much!
    This was an awsome practical explanation of FT I had ever seen.

  • @qinghuasong2166
    @qinghuasong2166 4 ปีที่แล้ว

    Awesome. The best explanation I find these days.

  • @ghpelectronics3405
    @ghpelectronics3405 2 ปีที่แล้ว +2

    Bo you are great!
    this is the best explanation of this topic I have come across and would absolutely recommend it.
    Well done!

  • @pussiestroker
    @pussiestroker 8 ปีที่แล้ว +1

    Pretty impressed with how much information (a collection of sine waves) can be encoded in a 2d plane via this construction.

  • @rafshantik
    @rafshantik 3 ปีที่แล้ว

    Wow...this lecture helps to visualise the concepts of FT ..such an amazing lecture.

  • @anaxprincessx
    @anaxprincessx 4 ปีที่แล้ว +1

    Great video! It was extremely informative and helpful. Thank you so much.

  • @hxong4733
    @hxong4733 3 ปีที่แล้ว +1

    Thank you for the intuitive explanation about Fourier transform! (So happy to discover this video omg

  • @nikitavladimirov5537
    @nikitavladimirov5537 3 ปีที่แล้ว

    Great explanation, very intuitive. A keeper! Thanks!

  • @user-ef3ej4pq4f
    @user-ef3ej4pq4f 5 ปีที่แล้ว +2

    This is pure gold man, good job!

  • @petertwiss356
    @petertwiss356 6 ปีที่แล้ว +1

    I agree wow, this IS the best description of 2D Fourier!

  • @balamuruganrajamanickam1262
    @balamuruganrajamanickam1262 7 ปีที่แล้ว +9

    it is really detailed FT explanation. thanks a lot.

  • @MukulKadel
    @MukulKadel 6 ปีที่แล้ว

    The best explanation of fourier transformation.

  • @chiahungmou8010
    @chiahungmou8010 3 ปีที่แล้ว

    Proud of seeing such clear explanation for 2D frequency.

  • @lamnguyentrong275
    @lamnguyentrong275 4 ปีที่แล้ว

    omg, best explanation:)) . i wish you were my professor. clear and simple

  • @andreavettiger5736
    @andreavettiger5736 5 ปีที่แล้ว +2

    Great explanation of FT! well done!

  • @waleedtahir230
    @waleedtahir230 7 ปีที่แล้ว +8

    This was awesome. Thanks!

  • @himalayansalt8385
    @himalayansalt8385 4 ปีที่แล้ว

    This is by far the best explanation I’ve found online regarding the frequency domain analysis of 2D signals. Great work!!!!!!!!!

  • @II-th7bn
    @II-th7bn ปีที่แล้ว

    Mind blowing, outstanding way of explaining ❤

  • @ElieElKhoury19
    @ElieElKhoury19 3 ปีที่แล้ว

    Such a great explanation. Thank you very much!

  • @mufcqw
    @mufcqw 2 ปีที่แล้ว

    your a king! cant belive this doesn't have 1M views

  • @thonghchau
    @thonghchau 4 ปีที่แล้ว +1

    Brilliance, thank you.

  • @1point689
    @1point689 4 หลายเดือนก่อน

    fourier transfer of fourier himself is such a nerd thing to do lol, love this lecture Thanks!

  • @tes8771
    @tes8771 3 ปีที่แล้ว

    Thank you for the clear explanation!

  • @irisobobo
    @irisobobo 2 ปีที่แล้ว

    Very nice explanation! Good job!

  • @deebadubbie
    @deebadubbie 7 ปีที่แล้ว +3

    Top class!

  • @alexandrevaliquette3883
    @alexandrevaliquette3883 9 หลายเดือนก่อน

    Let me guess... this kid learned proper English and proper physic. Congrat, another science hero sharing his knowledge with us!

  • @aminegourram2736
    @aminegourram2736 8 ปีที่แล้ว +3

    Very helpful!

  • @ryangeorge346
    @ryangeorge346 7 ปีที่แล้ว +1

    very well explained!

  • @formtwo3017
    @formtwo3017 5 ปีที่แล้ว +1

    this video is golden.

  • @coemgeincraobhach236
    @coemgeincraobhach236 3 ปีที่แล้ว

    fantastic explanation! thanks!

  • @lin2jack2
    @lin2jack2 7 ปีที่แล้ว +1

    Thanks it help me understand what is k-space

  • @mophy85
    @mophy85 5 ปีที่แล้ว

    Really helpful video~ Thanks

  • @jedt3d
    @jedt3d 2 ปีที่แล้ว

    Very clear and precise explanation of Fourier Transformation so far. Subscribe!

  • @taherimohsen2000
    @taherimohsen2000 5 ปีที่แล้ว

    Best video in Fourier transform 👍

  • @sharonwolf4019
    @sharonwolf4019 5 ปีที่แล้ว

    Great explanation!

  • @yangpu1043
    @yangpu1043 4 ปีที่แล้ว

    Very clear concept in physics to connect to maths

  • @jairotorregrosa
    @jairotorregrosa 5 ปีที่แล้ว +1

    It's a magnificent explanation, than you!

  • @mitchellsteindler
    @mitchellsteindler 4 ปีที่แล้ว

    Incredible video

  • @manepallysrikanth1383
    @manepallysrikanth1383 4 ปีที่แล้ว

    simply awesome sir

  • @shreyasgulhane5625
    @shreyasgulhane5625 6 ปีที่แล้ว

    Simply clever... keep it up...

  • @raymondzhao9557
    @raymondzhao9557 2 ปีที่แล้ว

    very well explained!

  • @nishantgoyal6657
    @nishantgoyal6657 3 ปีที่แล้ว

    Great job

  • @aaryaonlyone1067
    @aaryaonlyone1067 6 ปีที่แล้ว

    Thank you

  • @tdh5121994
    @tdh5121994 3 ปีที่แล้ว

    very intuitive!

  • @sara98habte97
    @sara98habte97 2 ปีที่แล้ว

    Love this

  • @das250250
    @das250250 3 ปีที่แล้ว

    A thorough understanding of the amazing fourier transform. The F.T in time will become the most poeerful tool in explaing quantum computing and construction fabric of the universe. It has yet to go in terms of its contibution to mankind.

  • @WinstonHo7
    @WinstonHo7 6 ปีที่แล้ว

    对我所听过的傅里叶变换的最佳解释!

  • @ERol-du3rd
    @ERol-du3rd 4 ปีที่แล้ว

    Good explanation

  • @dibakarpal2020
    @dibakarpal2020 2 ปีที่แล้ว

    💯/💯 Thank you so much sir

  • @amalalabarat3579
    @amalalabarat3579 3 ปีที่แล้ว

    thank you

  • @ronennenga9471
    @ronennenga9471 7 ปีที่แล้ว

    Thanks!

  • @MrGreen_N
    @MrGreen_N 3 ปีที่แล้ว

    Brilliant.

  • @jianjiejing4394
    @jianjiejing4394 4 ปีที่แล้ว

    really thanks

  • @WilliamAndregg
    @WilliamAndregg 9 ปีที่แล้ว +1

    Should it be a cosine at 14:27 (bottom equation) instead of a sine?

    • @mightbin
      @mightbin 6 ปีที่แล้ว +1

      i agree with you.
      if the phase delay is 2 WAVELENGTH, SIN will give a result 0, NOT A INCREASE OF IN TENSITY.

  • @sadikachoudhury7210
    @sadikachoudhury7210 2 ปีที่แล้ว

    Great!

  • @anshushaw3942
    @anshushaw3942 2 ปีที่แล้ว

    is the fourier plane the same as the back focal plane of a lense?

  • @CalebDiT
    @CalebDiT 8 ปีที่แล้ว +8

    First of all, this is a great video! Very insightful. I have a question about x, though. At 12:15, it's said that x=f sin a. Wouldn't this be x=f tan a? For small angles, I understand they'd be approximately equal. Is this done for convenience, then?

  • @ArtAutumn
    @ArtAutumn หลายเดือนก่อน

    Isn't there a mistake on 9:27? I think the Fourier image should be rotated on 90 degrees, coz in the direction of x there are many freaquences due to sharp steps.

  • @joehsiao6224
    @joehsiao6224 2 ปีที่แล้ว

    I might have missed it. Intensity of a point on the Fourier plane represents amplitude, but which visual feature represents phase?

    • @shirazkaderuppan3279
      @shirazkaderuppan3279 ปีที่แล้ว

      The phase plot isn't shown in this context. The FT plot you see here represents the magnitude; the phase is not easily interpretable.

  • @taoliu6334
    @taoliu6334 4 ปีที่แล้ว +1

    And at 15:25 I dont think it is Fourier Transform, it is Fourier series!! They are related but not the same!

  • @drmksu38
    @drmksu38 2 ปีที่แล้ว

    thank you very much . you dont have any idea how is that help me

  • @manudehanoi
    @manudehanoi 6 ปีที่แล้ว

    wow at 10:50 he shows the darkfield image !!!!

  • @peterpan8132
    @peterpan8132 5 ปีที่แล้ว

    振幅A可以通过点的亮暗在图中表示,那相位phi是怎么在图中表示的呢?

  • @luoboisme
    @luoboisme 11 หลายเดือนก่อน

    can anyone tell me why x = fsinα. is i miss something?

  • @kinsonl9015
    @kinsonl9015 7 ปีที่แล้ว +1

    Good job, man

  • @CL-sj5xl
    @CL-sj5xl 2 ปีที่แล้ว

    I had a high expectation at the beginning of this video, but when it goes to Fourier transform into frequency and inverse, I am not learning anything

  • @nadiaw1512
    @nadiaw1512 2 ปีที่แล้ว

    cool

  • @biomedicalscientistmuhammad
    @biomedicalscientistmuhammad ปีที่แล้ว

    kodous

  • @dimanabdullah8772
    @dimanabdullah8772 7 ปีที่แล้ว

    very useful

  • @Tigris_14
    @Tigris_14 3 ปีที่แล้ว

    9:32

  • @taoliu6334
    @taoliu6334 4 ปีที่แล้ว +2

    And at 17:36, he said "from previous slide, k would be f times sine of alpha" but previously he wrote "x = f sin(alpha)"...... listen to yourself professor!

  • @peili3936
    @peili3936 3 ปีที่แล้ว

    Despite the good parts, flaws are serious to me. In the beginning, the light propagates from the right to the left, but then the opposite in the slide with the portrait of Fourier. The meaning of sample and back focal plane is made confusing. And the definition of alpha is messed up.

  • @onescientist8468
    @onescientist8468 4 ปีที่แล้ว

    K_max has units of 1/length, by definition, and f*NA has units of length, you cant compare both of them, k_max=k*NA

  • @taoliu6334
    @taoliu6334 4 ปีที่แล้ว

    And and at the very end, he labeled the "sample" with "point spread function", how on earth could a psf be formed on the sample, instead of the image plane, when you were talking about emission??? Dont be disguised by his seeming confidence, the underlying logic was full of holes!!!

  • @taoliu6334
    @taoliu6334 4 ปีที่แล้ว

    I dont like the video especially because he made it very confusing when talking about the image formation with two focal planes. He didnt clearly define k there and I thought it was supposed to be about emission but he made it sound like illumination......

  • @elnora1469
    @elnora1469 7 ปีที่แล้ว

    great explanation thank you!