Yeast-two-hybrid screen (Y2H)

[@shemaiahmoore5904]

Y2H is so much easier to comprehend now. Thank you so much for covering this topic!

[@marwaatef4898]

Thank you so much for your clear and unambiguous explanations.

[@matthewmangus6929]

This video is a national treasure.

[@LeonEllisZ]

This is the best Y2H video I've come across that actually makes sense!

[@themoodz9490]

Easy to understand explanation. Thanks Henrik's lab!

[@ajahan9105]

Brief but clear enough to understand!

[@femiadepoju1389]

This is one of the best videos on Y2H. Thank you for making this.

[@blountyhunter1966]

Great intro into Y2H, clear and concise! Thanks!

[@medielijah]

Clear, concise, helpful! Thanks!!

[@grayceseeto]

Helped in my understanding thank you

[@alpaslanaksoy533]

Thank you very much Henrik! You are doing a wonderful job with your videos.

[@henrikslab]

Thanks!

[@sinamt2982]

Vielen Dank, das war richtig gut erklärt!!

[@peerzadazubairahmad464]

clear very much

[@alexandertomlinson4517]

This was so helpful as I am doing a project right now. Thank you!!

[@Unokatziba]

This is the best Y2H video on youtube - so easy now to understand, thanks a lot!

[@swativerma6983]

You are a life saver. Thank you

[@hadileghaddar]

So clearly explained! Thank you!!

[@pallavikamble2931]

Very well explained!!!thank you

[@weightelk]

Awesome, thank you for the clear explanation

[@bioxplaining_by_anuska]

Very nicely explained.

[@ChinchillaHappyLife]

Very clear explaination! Big thanks

[@nagylottii]

Thank you so much, it helped me so much!

[@ilknurargum3326]

Thank you so much, ths video helps me a lot!

[@graemelaubach3106]

Very helpful, friend. Thank you muchly!

[@wahahacoke]

Best explanation ever!

[@kki5061]

Thank you. Wonderful explanation.

[@user-oi1ds3hk2n]

It’s very helpful, thank you so much.

[@popitrock20]

thank you so much, so clear and concise!

[@Kaffeetasse9]

danke! endlich mal ein verständliches video :D

[@ponktiaddrita6274]

Thank you so much.

[@Ashu-hy7br]

Just amazing👍👍👍👍

[@purplesloth94]

Thanks so much for this helpful video!

[@thathzu2961]

Thank you ❤

[@juliariordan6082]

Great video thanks!

[@suriethaesterhuizen1802]

This is a great explanation, thank you so much!

[@lillininaw]

Richtig gutes Video, vielen Dank!! 🙏🏼

[@henrikslab]

Freut mich wenns geholfen hat!

[@lanaamin3605]

Thanks alot, it was very useful

[@sajasamy8891]

Well explained thanks ❤❤❤

[@aliilchizadehkavgani6663]

very good explanation, thanks

[@killianpapail9324]

thank you very much bro

[@jacobkiriakos8321]

Thank you

[@vinodsinghbhojak7383]

amazing

[@55prasenjit]

Excellent explanation ❤

[@Rosessansepine]

Thank you it's really clear !

[@satyavadlamani9420]

Nicely explained. Thanks a lot! Is there a particular transcription factor used for this? Or a criterion for choosing the transcription factor?

[@henrikslab]

Good question! So the main criteria is that you have to use a strong transcrption factor which initiates transcription of your reporter gene. In that case here they used Gal4p transcription factor: link.springer.com/protocol/10.1385/1-59259-762-9:247

[@zalim2productions]

Very clear thank you so much

[@swayambasu8597]

Hey thanks a lot for this brilliant video but I have a doubt here.... we take 2 components of the transcription factor AD and DBD and bind the 2 proteins one to each.......how can we say the function of the 2 components does not get altered when one of the proteins is attached to it? Pl help me understand

[@sevodar]

finally i got it .
thank uuuu .
Allah bless you ❤

[@cloudtheavegner1000]

RNA Polymerase binds to the promoter and transcription factors bind upstream of the promoter.

[@futurogelatina4395]

Thank you so much!

[@qny1286]

clear explanation

[@subhankarsinha448]

Nice presentation, thank you, make more videos on rdt

[@achmadsyarifudin2976]

Easy to understand good job

[@meghanadhbanavath8850]

Good explanation

[@Kaur_2343]

Thnx a lot sir...it helped me a lot in undrstanding this concpt💖💖💖

[@henrikslab]

Perfect!

[@Jake_M_Warren]

wow that's a very elegant system. Does it mean then that the proteins need to be taken up into the nucleus in order to be tested, is there limitations in terms of size and charge?

[@Biochem656]

Thanks a lot

[@annarauchberger7815]

Thank you!!!

[@user-er6mu5se6m]

U r great

[@b.a.sbadassugar5007]

Every other source i found doesn't mention anything about Gal4AD recruiting the polymerase, they just mention that transcription happens. Do you happen to have any sources that show that GAL4AD does indeed recruit the polymerase?

[@marinbelz3448]

GJ!

[@pablonomas]

love u

[@nekoschneko7145]

danke Bruder ich küss dein Auge fürs video

[@matevzkosutnik7573]

OK so i have 2 questions i hope im not to late
1. how can you bind a protein to DBD or AD
2. what are the usses of this
also i very much like your channel

[@henrikslab]

Matevz Kosutnik thanks for your question
1. you synthesize a DNA fragment where both sequences are connected and encode a protein with is ´chimericˋ meaning a novel protein out of two individual proteins which are linked via peptide bonds.
2. you can test whether protein A is interacting with protein B (if so, you generate a reporter gene expression)

[@aaronb2547]

Hi nice video!
Hab eine Frage
Also dient dieses System lediglich für die Erkenntnis ob Protein a und Protein b interagieren?

[@henrikslab]

Ist im Normalfall wirklich ein Verfahren um Interaktionen von zwei Proteinen zu checken, ja!

[@londonberry2180]

Does the bacterial adenylate cyclase two-hybrid (BACTH) system also utilise this concept?

[@anupamunnikrishnan8727]

i love you

[@henrikslab]

You can discuss this with my girlfriend :D

[@tanvirshovon8246]

Ooooooossaaaammm

[@matteobarca1050]

Thank you so much! I liked your video and it was at 666 likes, so sorry if i ruined it!

[@henrikslab]

You did not! The goal is definitely a 1000 likes :D

[@deepakmalik3746]

Thank you

[@suhailahmad4120]

Thank you