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here is a question! if the methylation is saving the prokaryotic DNA to be cut be by the restriction endonuclease, how can we cut some DNA of interest of a prokaryote by a restriction enyzme? Will the enzyme be able to cut the DNA of interest in the presence of methylation?
Mam I saw many online lecture But your teaching is so beautiful You lecture take less time and covered all concept with better understanding Thanks ma'am your teaching help so... Thank you thank you
Because MS can recognize its cells DNA strind( bachterial ).it works for endonuclease by diffrentiat its cells dna by methylates to it. Thats how endonuclease cuts foreign DNA .
1) Mam can alkaline phoshphate enzyme remove OH also from the genetic material or does it remove poshphate only? 2) does methyl group gets attached to the N2 bases of host's genetic material only?
Visit www.neelabakoretutorials.in for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Sry for interruptions 😊... As I know... Vectors have multiple restriction sites but an endonuclease is confined to cut the chromosomal DNA only at a specific site...( like either blunt end or a staggered cut) ... Coz for this unifying feature , a particular endonuclease is allowed to cut both the gene of interest and vector...
@@MuhammadYaseen-dd1uw NO... as hydrogen bonds are present between two strands of dna ..they are not broken...because..here we are seperating the G and C ...which are present complementarily in the opposite strands...as the dna is seperating at the same place in both the strands...hydrogen bonds need not to be broken...
@@KurapatiKvsnRaju as hydrogen bonds are present between two strands of dna ..they are not broken...because..here we are seperating the G and C ...which are present complementarily in the opposite strands...as the dna is seperating at the same place in both the strands...hydrogen bonds need not to be broken...
Mam u said that ligases are needed to make phosphodiester bonds....Then why alkaline phosphatase is used to avoid bond formation......If we dint provide ligases ,they will remain circular ..... Correct me If i am wrong... Thank you
Prakash Raj Bro actually we need phosphodiester to protect its own joining again but whe have to join it with inserted DNA so first we replace it again we put it to join with the DNA that we put there... hope its clear.
Mam ek chota sa correction hoga shayad..wahan the roman no.indicates the order in which the enzyme was isolated frm the strain of bacteria..nd HindII first discoverd not first isolated hoga
*Hello Ma'am, Name the Four Nuclease Enzymes Each For Cut the Sticky End At 5prime And 3prime Ends And Four Enzymes That Cut At The Blunt End With Their Recognition Site*
Mam but why can't the methylated group be attached to the A and T of foreign organism while the endonuclease can't differentiate between self and non-self
Your lectures are very clear , easily understandable and without scribbling anywhere on the white board. After the end of the lecture if one sees the white board it your lecture when read should be followed. Sorry to point out one flaw when you draw a straight line below the Binomial nomenclature of any plant or animal or prokaryotic bacteria.Separate line should have been drawn below the generic name and a separate line for a specific epithet. I really enjoyed listening to your lectures.
I think you switched between first isolated and first discovered...because it’s clearly written in ncert that Roman numbers are based on order of isolation
even though u r speaking english im feeling that as u r speaking my mother tongue.u r telling in that easy wayy.... hatsoff 2 u madam jeeeee.....
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for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
The real teacher on you tube for biology
Mam your teaching style is awesome.
Thank you very much Madam you make our basic ,stay bless
Mame way of teaching is really overwhelming and elegant .you clear the concepts like a crystal
U r explaination is really awesome Ma'am..... Thanks a lot.... It's really helpful...
Thanks from side of ma'am
Polimer news live
Hi Nancy
Thanks for teaching the most difficult chapter...in such a simple way....
Mam ji its supr sai bhi upr really too much easy to undrstand 😇😇😇
Your effort is amazing
For all of us premedicos.
THANKS MAM
THIS VIDEO MADE MY CONCEPTS STRONG
Mam u r very good teacher I have ever seen
I am very grateful to you ma'am ❤🙏...excellent teaching ..everything is clear now
the way u draw diagramss do beautifully and in a very understandable way. YOU ARE LITERALLY AN ANGEL SENT FROM ABOVE FOR NEET ASPIRANTS😭😭
A clear explanation comes only when person himself has clear concepts... u r da best exmpl mam...
Amazing mam. I hope you would be awarded for best teaching. Really very well understood. Thank you.
The revolutionary teacher🤗...neela mam🙏
thank u so much mam....ur teaching is awesome ...hoping more n more videos from u....loved ur teaching way 😍😍😍😍☺😊😊
You are Excellent mam..Thank you so much for enhancing our knowledge by making such online platform.
Maam u r an angel in this lockdown period to all kids of class 12
really mam i wish .. u were my teacher 🤗🤗🤗
ur lectures are awsome mam... thanks a lot mam..
THINK WITHOUT InK
here is a question! if the methylation is saving the prokaryotic DNA to be cut be by the restriction endonuclease, how can we cut some DNA of interest of a prokaryote by a restriction enyzme? Will the enzyme be able to cut the DNA of interest in the presence of methylation?
Best teacher 😘😘😘
The best teacher on you tube for bio.
13 Dec 2020
Hello ma’am!
Why is it easier to add insert on sticky end than on blunt end?
@@kalendersheriff8744 oh ok thanks for the info
@@kalendersheriff8744 thanks a lot
Best way of teaching in the most simple way
Mam I saw many online lecture
But your teaching is so beautiful
You lecture take less time and covered all concept with better understanding
Thanks ma'am your teaching help so...
Thank you thank you
Mam, Why methylating system do. not add methyl group in the virus genetic material? Is it differentiated by MS?
Same doubt
Because MS can recognize its cells DNA strind( bachterial ).it works for endonuclease by diffrentiat its cells dna by methylates to it. Thats how endonuclease cuts foreign DNA .
In short ,MS have ability to recognize its cell's DNA.
@ANUSHKA MISHRA thank you so much.... Anushka mishra
@@surendrasharma5677 and u too thanks
Thank you so much mam ❤️❤️
thanx mam MaY ALLAH give u long life full of hapiness....Ameen
Thank you mam for making the concepts more clear.
Thankew mam fr helping mee sty always blessed 🤗😊
mam bacterial DNA have Uracil in place of Thymine mam
1) Mam can alkaline phoshphate enzyme remove OH also from the genetic material or does it remove poshphate only?
2) does methyl group gets attached to the N2 bases of host's genetic material only?
Thank you so much for these awesome videos!
I have a question, at 8:00 is there a mechanism that allows the cell recognize which A-T to methylate?
Restriction endonuclease have Restriction-Modification system.
Just watching 👀 lectures...feels good 👍
Thanks ma'am ❣
Apkai padha bilkul apki andaz se match karta hai ❤
Amazing. .. lecture maam...soo helpfull. ... ! 😊
Best biotechnology teacher you clarify all my doubts of this topic 🥺 we love you Ma'am ❤️
Visit www.neelabakoretutorials.in
for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Too gr8 teaching madam jiii🙏🙏🙏🙏👍
ma'am ur teaching is excellent....thank you for providing such amazing videos!!
Thank you Mam for teaching things in an easy way
Thanku
Thank you Neela mam
PERFECT EXPLANATION !!
THANKS MAM
Thanku very much mam ur teaching skills are ossum I was very much confused with this topic but after watching ur session I got a clear picture of it
Same here
nice teaching....
tnq maam...u r vdo hlps me a lot
Mam how will endonuclease correctly adds the methylating group to its own dna it can even add it to phage dna right ?
endonuclease does not add methyl grp, methylating agent adds methyl group
In NCERT, it is written that the Roman numbers indicate the order in which the enzymes were "Isolated". Please check.
THANK YOU MAM ❤️
mam use orange marker instrede of red marker orange colour looks clear then red colour please nice teaching u are having mam
your class is soo super and soo understanding one ...thank you soo much mam
Does each endonuclease have a unique property of cutting at different sites and how does this done?
Kindly clarify my doubt, ma'am.
Sry for interruptions 😊... As I know... Vectors have multiple restriction sites but an endonuclease is confined to cut the chromosomal DNA only at a specific site...( like either blunt end or a staggered cut) ... Coz for this unifying feature , a particular endonuclease is allowed to cut both the gene of interest and vector...
Thnku mam ur teaching style is very nyc it helped me very much to clear the concepts thnks a lot mam☺
Thank you ! :)
Mam so much thanks ...bhot ache se aap ne mere concpet clear kar diye ...so much thanks mam..........
Amazing lecture mam.... Thank u
Thank you mam for this amazing session
In second case of palidrome sequence, why hydrogen bonds are not broken. Pls reply
Mam is gonna wrong in that case there is hydrogen bond between c and g which is cutter while the line shows phosphodiester bond
Can you plz explain it mam...??Even btwn G nd C there exists hydrogen bonds right??
@@MuhammadYaseen-dd1uw
NO... as hydrogen bonds are present between two strands of dna ..they are not broken...because..here we are seperating the G and C ...which are present complementarily in the opposite strands...as the dna is seperating at the same place in both the strands...hydrogen bonds need not to be broken...
@@KurapatiKvsnRaju as hydrogen bonds are present between two strands of dna ..they are not broken...because..here we are seperating the G and C ...which are present complementarily in the opposite strands...as the dna is seperating at the same place in both the strands...hydrogen bonds need not to be broken...
Excellent.....👌👌👍
I have a doubt about, in sticky cut two types of bond are broken and in blunt cut only one type of bond phosphodiester bond broken how?
Great teacher . Thumbs up !
ur teaching is awsm......,mam
I am also a biology teacher yet you are my mentor ma'am...i heartily salute you 🙏..hope one day i would also be helpful as you are for the kiddoss
Visit www.neelabakoretutorials.in
for a well planned road map to help students score 360 marks in NEET
GREAT JOB mAM THANK U SOOO MUCH
Mam u said that ligases are needed to make phosphodiester bonds....Then why alkaline phosphatase is used to avoid bond formation......If we dint provide ligases ,they will remain circular .....
Correct me If i am wrong...
Thank you
Prakash Raj Bro actually we need phosphodiester to protect its own joining again but whe have to join it with inserted DNA so first we replace it again we put it to join with the DNA that we put there... hope its clear.
Underdog thank u....
Ur teaching is really awesome
all lectures are very good
Your way of teaching is simply fantastic
Mam ek chota sa correction hoga shayad..wahan the roman no.indicates the order in which the enzyme was isolated frm the strain of bacteria..nd HindII first discoverd not first isolated hoga
Mam if that lysozyme enzyme kills the bacteria then could we isolate the DNA???
excellent mam.......
Awesome lecture. Cant thank you enough 💗💋🙏
😂😂😂2nd emoji of urs lol
Yaa 😂🤣😹
Thank you mam .
Great lesson ❤️
thank-you mam your lectures are osm
THANK YOU MAM
mam your teaching is just awesome..can understand concepts very easily...btw mam can you tell me how we dissolve the nuclear membrane
Superb explanation👌👌mam
your classes r indeed helpful
Are these lectures for Neet aspirants mam?
*Hello Ma'am, Name the Four Nuclease Enzymes Each For Cut the Sticky End At 5prime And 3prime Ends And Four Enzymes That Cut At The Blunt End With Their Recognition Site*
thanks a lot mam .. 💐💐💐
mam i have a doubt on pallin drop u gave a example on gaattc by using any word we have to use the same method?????
Mam, how would we get to know about the discovery of bacteria???? 🤔🤔🤔🤔🤔🤔
Plzz tell............if some other one know the answer guyz!!!!
Thank you so much ma'am 😄
Pls allah! keep her alive for 200 years.. coz this earth needs her contribution..
Superb lecture s maaam.....
Thanks madam 👍
Mam you're too good
Mam I have a doubt
How do you giving the code name for E. coli and haemophilous influenzae?
Mam but why can't the methylated group be attached to the A and T of foreign organism while the endonuclease can't differentiate between self and non-self
u r lectures are awsome mam
Excellent work
Tq so much mam😃
very nice teaching style, thank uuuuuuu mam......................
Your lectures are very clear , easily understandable and without scribbling anywhere on the white board. After the end of the lecture if one sees the white board it your lecture when read should be followed. Sorry to point out one flaw when you draw a straight line below the Binomial nomenclature of any plant or animal or prokaryotic bacteria.Separate line should have been drawn below the generic name and a separate line for a specific epithet.
I really enjoyed listening to your lectures.
Pandian Tt I love you
I love your style
Very good videos
mam you teaches very well
I think you switched between first isolated and first discovered...because it’s clearly written in ncert that Roman numbers are based on order of isolation
Mam 1 thing i could not understand is that from what is the endonuclease been isolated from ??? From the virus ??
Bacteria dude
Thanks mam for concept