Preparing Solutions - Part 3: Dilutions from stock solutions
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- เผยแพร่เมื่อ 20 ส.ค. 2024
- This tutorial describes how dilutions are made from stock solutions, and how to calculate the volume of stock solution required for a given final concentration. The rules here apply equally whether concentrations are being expressed as molarity, %, mg/ml or other.
thanks for restoring my confidence, i was the most confused person in lab regarding calculations. again thank you so much Mam
same situation.
You're not alone 🙂
concentrations have always come so hard to me compared to other parts of chemistry, no idea why besides that i'm just less math oriented
This video has got me through my degree
I really need to thank you for this tutorial, a total of about 6 years in school and counting. I lost confidence in myself until I came across your video today. Thank you so much.
I usually forget these calculations again and again but I thank her because every time I knew where I need to learn it again when I do forget.
This is hands down the clearest explanation ever! Better than any website!!!
I'm currently doing my second year in Biomed, I suffer from dyscalculia. This video has saved my grades! I've never felt so comfortable in labs and exams working out buffers etc. Thank you.
Thank you very much.. never knew how to do it before; never will forget how to do it from now on
Thankyou mam i have watched all three of your videos and my concepts are clear like never before thankyou so much v apprenticted your work now i am able to solve any type of sims
This triples your work. Use M1V1 = M2V2 and you'll solve these in less than 15 seconds.
Example: Stock solution of 70% ETOH. How to make 100 ml of 10% ETOH?
(70%) x (volume) = (10%) x (100 ml) --> Volume = ((10 x 100)/(70)) --> Volume = 14.3
Thank you so much. I've been searching for too long for how to do the dilution method and this helped me quite alot.
Can you please make more videos? I could listen to your voice all day
I am so glad to study in Glasgow Uni and waiting to meet you in person, I would like to know what is written in the board in the back side about Phd, this is what I am planning to do next step, Thanks a lot for your lovely explanation
The equation made my life much easier. Thank you
A very good step-wise presentation. Please, more its kind.
Just made my life so much simpler
Very well explained mam. very helpful video.thanks for sharing
Please keep making videos! Very helpful. :)
Great trick
Excellent explanation
Thank you!!! Great explanations for all videos
thank you very much, best explanation ever
by the way you are so pretty
Very helpful
Can you please do a video for serial dilutions please?
Thank you so much! The concept is clear for me now.
i should study in glasgow.. u made it so easy to understand, thanks alot !
Plz make more videos mam.don't stop
400ml to make 2%.
1 part in 5 = 200ml
Half of a part = 100ml
So to make 1.5% solution
I would need 300ml per 1000ml
I think.
You can do C1*V1=C2*V2 in doing so you get X*2%= 1.5%*1000ml and if you solve for x you get 750ml of 2% stock soln in 250 of water.
but according to this video you would want to divide (1.5%/2%)*1000ml= 750ml of 2% stock soln in 250 of water.
Please which University are you a professor at you are a good teacher keep up the good work
OMG, Sophie Marceau doing molecular biology!!!
Hey, I still do not understand how can I apply this when for ex. I have to make a solution 1:1000 for 50 mikro M fiinal working solution, when I have no other information like volume.
That was super useful, thanks.
So helpful, thanks alot
Thank you very much!!! incredibly useful
Thank you so much for your help!
Your videoi is made so simple,Thank u so much ma'am,Can you please help me with serial dilutions and back calculation
im in love. thank you sooo much for this
so theoretically, if I wanted to pippet a sample in a lab of 5.56microL, I would use a P20 with a 055 reading but what about the remaining .01microL, or would a "pipet W/ bulb and cylinder " be the only way to accurately draw a measurement in the hundreds place?
Thank you so much!
Hi ma'am, very helpful thank you. Does this kind of dilution (ie. starting from a stock) count as serial dilution? Or is serial dilution only when you keep decreasing the concentration by a set factor.
Thanks in advance.
It was very helpful. Thank you so much :)
brilliant video thank you
Mam u teach sooooo welll thank u
Thanks you so much clear , and usefull
best ever..Thankyouu!!
Please explain the calculations for enzymatic digestion of dna
amazing !!!
thanks for explaination
i get it now. Thank you
please help me out of this: I need to use 100ng/ml of activinA(26 kDa mol. wt ) in 40 ml media.
what concentration of activin A i need to use to make it?
Thank you so much...
thank you so much!!
Many thanks
Thanks so much
Stock concentration of IL-1 beta = 25 ug/ml
Need to treat cells with 1ng/ml IL-beta
Each well of cells in a 96 well plate is treated with 100 ul of the IL-1beta solution
You need to make up sufficient volume to treat 6 wells, using cell medium as the diluent.
Questions
Q1: what total volume of IL-1beta solution do you need to make up
Q2: how much of the stock solution do you need?
Q3: how much of the cell medium do you need for the dilution?
CAN ANYONE HELP ME? I DO NOT KNOW HOW TO DO THIS AND HAVE BEEN GIVEN IT FOR TOMMOROW
Thank you
I am always afraid when it comes to calculations in biology 🥲
Solve That
0.01 M sodium phosphate buffer in 20% sucrose: dissolve 0.095 g of acid (NaH2PO4), 0.054 g of salt (Na2HPO4), and 20 g of sucrose and make volume up to 100 mL.
Babe.
Thanks so much
Thank you very much