How Community Science Experiments Can Change the Reefing Hobby |
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- เผยแพร่เมื่อ 14 ต.ค. 2024
- In this session we chat with Dr. Eli Meyer and Dr. Andrew Bouwma about the first ever Reef Builders community science experiment.
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The longer I keep my reef tanks the more I realize you just can’t beat water changes
You can also look at apigenetics. Where different genes are turned on / off depending on environmental factors.
Currently work at a LFS/Maintenance company. Am currently having an argument if using activated carbon causes HLLE. So an investigation on what causes HLLE would be cool.
You guys, or just Eli, should create a selection of sku’s dedicated to different experiments. So you could go to Aquabiomics and purchase the “carbon source experiment bundle”. Comes with the texts, carbon source, rules/directions etc… then as more experiments are started anyone can add to whichever experiment they want
Interesting ideas, I understand the need to get a sufficiently large N but normally great effort goes into making those replicates the same to avoid too much noise in the data. Even though your sample size may be large the variance within your samples is huge, tank size, fish load, coral species, salt mix the list is almost endless. Look forward to seeing the results and how you interpret the data.
I really enjoyed this episode. Thanks!
I have an answer for the protein transfer question.
Yes there is an instance of protein transfer between two separate individuals. Kinda.
In solid organ transplantation there are three mechanisms of allorecognition, allorecognition being the detection of non-self by immune cells, namely T cells.
1 - direct. Recipient T cell recognizes nonself MHC molecules on grafted organ itself. (simply put, MHC is the self-identification molecules, different MHC = non-self).
2 - indirect. Recipient T cells recognize parts and pieces of of non-self MHC presented by Recipient antigen presenting immune cells
3 - this is where the transfer comes in.
3- semi-direct. Non-self MHC is released from the graft in extracellular vesicles l, such as exosomes or apostolic bodies. These intact donor derived proteins are then acquired by Recipient antigen presenting immune cells and thus activating Recipient T cells.
Between species does this happen? Maybe... I haven't seen the xenotransplant data on exosomes and semi-direct recognition. Probably doesn't with the new GMO pig organs being used.
Back to coral
However, for the transcript factor theory to hold you have to consider it's almost like an enzyme, in that its target gene promoter region will have to be of high sequence homologous for it to transcribe it's target. That's before we even get into chromatin accessibility conversations too.
I remember Jake talking about the whole green fluorescent infection and he was hypothesizing that it was some sort of rouge prion that makes its way into the coral. But again who knows
Would like to see which Coral dips are the most effective, and how many different dips should be done throughout quarantine.
I tested what’s in every dip but I have to secure intellectual property from my school to release that information - it would be interesting to test which dips have what affect on the microbiome
Really looking forward to the results, good stuff microbial men!!
So where are you going to measure the microbiome -only from the tank water samples, or from samples collected from surfaces (live rock and/or sandbed)? Rwlying on water samples will not answer much.
Hi. Aquabiomics sequences both the water column and a swab sample, which can be utilized to measure biofilm communities. We will have consistent sampling instructions telling participants in the experiment to only swab the sand, rock, etc, to keep things consistent. Additionally, carbon dosing experiments were done in 2010 by Dr. Sanjay Joshi and Dr. Ken Feldman, where they added a carbon source and then quantified the number of bacterial cells in the water column using flow cytometry before and after. It was found that after adding a carbon source, there was a significant increase in the number of planktonic bacteria. Thus, we expect sequencing the shifts in the water alone would give us much information (but the results will also include a surface sample).
Cool, sounds like a good plan.
Would be also really interesting to know if the tank microbiome is a representation of the fish gut microbiome, being a closed system (and a fish toilet) like the tank is. My guess would be that the different types of foods (coral and fish food) might have a different kind of effect on the whole tank microbiome, at least being one of the confounders.
Maybe the most efficient way of getting those beneficial bacteria out in the watercolumn is by disturbing the sandbed during water change (Andy's observation) 😊
Remy, do you run your reef matt 24/7? I only run mine for 12hrs a day so it doesn’t tank my po4/no3
what is the source of carbon you're going to use for this experiment (Qstn for Salem) and what if differant carbon sources effect differently on the bact population
We will be testing the affect of vodka, vinegar and amino acids and how they affect the bacterial populations in our tanks. Once we have the data we can determine if these carbon sources have similar or different effects on the microbiome.
@@salemclemens6148 looking forward to the results
"Maybe theres new science(Biology) here"
When a scientist wants to say its probably bullshit 😅
I truly admire all the knowledge shared, but this is not one of those subjects reefers want to hear, or benefit from. Maybe for the scientific community, or those few reefers involved on the scientific aspect of it. Thanks for all you do for the hobby.
Shame what you've done to this podcast