I bumped my head and put the blood under the microscope
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- เผยแพร่เมื่อ 19 ก.ย. 2018
- One drop of blood contains billions of blood cells. You can see the red blood cells (erythrocytes) quite well. In this video I show you how I put blood under the microscope and look at it using various contrasting techniques. I bumped my head on the corner of the car door unloading some shopping. [Video: 060]
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Loved that intro bit 😂 Not too many people hurt themselves and instantly think, “Quick! To the microscope!”
I was already about to wash it off, when I remembered that it might make an interesting observation...
When I was bitten by a spider my first thought was 'This is interesting, I hope I can photograph this before it lets go.' Luckily it kept biting me and I was able to get my camera and attach a macro lens and photograph the spider.
Spiders in the UK do not usually bite, so I wanted to identify it.
I sent the photo to a specialist who told me that it was _Segestria_ _ florentina_ a Southern European species that has become naturalised in port cities in the south of England.
Honestly I remember scraping my leg and wanting to check it under my microscope!
XD
Similarly but not even a few people who hurt themselves pick the microscope and film the fucking experience and start the show. That would be a calming experience if all people who would rush in the hospitals think that first along the lines.
You really don’t have to hurt yourself for us XD
*clicks the video*
"just bumped my head on the car door"
I love how you turn moments from your everyday life to microscopy videos!
All the additional information well appreciated. As with every bit of knowledge - the more, the better.
I try to move microscopy and science exploration away from the lab into everyday life. I am a biology teacher and I often feel that the stuff that I have to teach is somehow disconnected from "real life" - a lot of theory. This is one of the reasons, why I deliberately try to dedicate more time in the videos to the environment from which the samples are taken, the "everyday life", so to say.
On the note of "Biology being disconnected from real life", it is so ironic, isn't it? Biology is all about life!
I, too, didn't quite pay a lot of attention to biology and other subjects full of theory in school. It was only eventually, through curiosity and world exploration, when I realized how sciences are applicable to everyday life and related to each other.
What you are doing is great, we need more practical, real world examples to awake curiosity in children and show them how the knowledge can be used and help them understand the world we live in.
@@Microbehunter can we see corona virus under 2000x magnification
MH -- next time, use a blood smear! I believe it is the standard technique and very easy to do! Put the blood towards one side of the slide and then with another slide at an angle, touch the blood drop so that it spreads along the edge of the slide at an angle and then slide the slide (oops) along the surface of the horizontal slide. You will get a nice tongue shaped smear and as a bonus you will find that the larger white blood cells are more abundant at the *tail* of the smear and the platelets and red cells are at the base (starting point of the smear). As an aside, for parasites, such as malaria, a *thick smear* is utilized for which 2 drops of blood are placed and the same slide technique is utilized. Proper staining helps the identification of the different normal cells and parasites, of course! :-)
You banged your head on the the car door, ouch! Another great video though, keep them coming :)
every time you touch this theme, my respect for you drops a notch.
have touched, and it has been that way is more correct. can't know the future😊
Oh wow, you’re a perfect host. This channel is definitely going to take off!
youre the best. please keep being yourself. you are a great person
I love phase microscopy. It just gives such depth without staining requirements! Have you ever used AmScope products?
Not everyone will think the way you think just wow.. you are really committed to teach.. doing everything for science 😊💞
Water should be normal saline or something isotonic. Otherwise...bloated, lysed or shrunken cells!
just what i was thinking! where's the saline!
Your are crazy, very nice channel, what I was looking for. Thank you for the hard work and great stuff.
I love these types of videos 👌 thank you for creating such great content ☺️
- the video started with an open wound
- You sacrifice so much for my knowledge curiosity :-) thank You for creating, this is a relevant topic for me (new starter with microscopy) 😎👍
About bumping your head.. Ouch. Also i love your channel and the theme of your channel about that small world under the microscope.. Amazing!
Love the content❤️❤️❤️
thanks for sharing. I like the phase contract footage.
You may not believe that you can do a diagnosis looking at the blood but you can certainly tell if it and the person are healthy or not!
- very good 👌 when You are telling magnification level each time - this is small but very important information 😎💯
Fascinating thank you for the information
Great video! Cant wait to hear how you're liking the phase contrast set! ;)
An interesting point about rouleaux. The glycocalyx of erythrocytes (red blood cells) is negatively charged, causing them to repel other red blood cells, and this aids the cells to avoid obstructive aggregation and to move freely around each other. This repulsion may be cancelled out by the high presence of positively charged species, such as proteins. For example, if there are higher than normal levels of circulating immunoglobulins, their positive charge can interfere with with RBC-repulsion due to the higher concentration of cations in the blood. This is actually the principle of a test called an erythrocyte sedimentation rate, for in vitro diagnostics, which is a non-specific indicator of inflammation.
When blood is anti-coagulated, rouleaux formation on a blood slide may be due to high cationic concentrations.
Wow, yu good. Great info on blood cells repeling each other. Becasu with covid they are not anymore.
This happens in Multiple Myeloma, thank you for the information :)
Yesm thank you for the clarification. Viewing blood under a microscope DOES have diagnostic value, contrary to what our esteemed host claims. It's part of the toolset of the Meducal Lab Scientist, one they use most every day.
Thanks ☺️
Try Earthing - it will break up the stacked RBC formations and let the RBCs flow independently for much better blood circulation.
Pure devotion. Bliss
Just found your channel.. good job 👍
This man is hilarious. "Ouch I bonked my head... quick to the microscope!"
instant subscriber ahahahah a job well done mate
Every time I hurt myself I instantly go to my microscope! Love that your brain works the same way!
This guy is a seriously hardcore enthusiast 😂
Microbehunter: someone shot me with a shotgun, time for microscope.
Great video, now we have an idea what we can expect to see with a light microscope (and what we can not see). It would be very helpful to give details of equipment you used for shooting the video and how it would compare to the results of these cheaper scopes with dubious 2500x magnification claims. Say, compare a Nikon at 40x to an image from a cheaper AMscope at 40x, etc. Thank you again.
Love that intro.
Nice video ☺️ i
THANKS man that was AWESOME!!!😆😆😆😆😆😆😁😁😁😃😃😃😃😃😄
This dude is a legend
Just love the way you make your videos keep making the videos
Tell me which microscope is budget friendly and best for students 🤔🙏
check out foldscope
Is there any solution or nucleic acid to see white blood cells?
Im really curious about it.
WOW EXCELLENT!!!!!!!!!!!!
My dude went whole another level on research..
:DDDD YOU MADE MY DAY
It's amazing
Wondering what causes the slight oscillations in the dark view once the flow has settled? Brownian motion?
did you just bump yourself to your car door?
*use flex seal to seal that *WOUND!*
Hi friend is this Microscope good for blood cells [ Swift SW380T 40X-2500X]?
Im new, and looking to buy a microscope, is there a cheap but great enough, microscope you can suggest?
Nice... brother
I may have blood also. I’ll check next time I get cut. I may still be running on maple syrup though.
Can you check your blood out now after vaccination and see if there’s any difference?
Lol - I do that too. I wait to get hurt to see it under the microscope
heyy! can you do a blood of an engorged tick? I really want to see it.
what about tick borne infections..you can see the infections within the cells?
hallo how are you I am Ashraf thank you for ur hard work
Most people: ouch I'm bleeding imma get a bandade
People into microscopes:
Yah I'm bleeding let me go get a slide
It will probably clot by the time we get to you. Well perhaps you can section the clot and enjoy a glimpse into how blood cells form a mesh and stop the bleeding. However, if inside a blood vessel, I suggest the victim runs to the ER rather than reaching for a slide and his or her microscope
You should have done a blood smear instead of mixing it with water. That's how we're doing a part of analysis in medical school. And you'll get a nicer view of all blood cells too.
I think he did see something Abby Normal. you can hear a pause in the voice with a silent 'gasp'! as soon as video ended, he calls CDC for consultation
"I just got hit by a bullet so it's a good opportunity to checkout some red blood cells !!" :')
What do you mean here, when you say that observing blood has no diagnostic value? I'm confused? You mean at this magnification? Or without certain stains? Because we all know that observing blood cells can lead to several diagnoses. Such as Anemia, Thrombocytopenia, sickle cell anemia etc.
Also, this brings me to my next question, let's say I wanted to perform a blood count, is that possible with a low-grade microscope? What would I all need to accomplish this other than say a Hemocytometer would I need special stains? Would this be even possible using amateur equipment? The reason I ask is I'm interested in the blood cause I was diagnosed with AML Leukemia now in remission. I've learned a lot about blood just researching and being a patient. Also what magnification and things are needed to see platelets?
In brief, I refereed to the so-called "live blood analysis" using dark-field, which has no diagnostic value. You can use microscopy to observe some parasitic infections, do a count, etc. but this is not what I meant becasue these things are directly related to the blood. In "live blood analysis" they want to be able to diagnose other diseases as well, but this is clearly unscientific. en.wikipedia.org/wiki/Live_blood_analysis
> with a low-grade microscope
If you can see red blood cells then this works. If you can not see red blood cells, then the microscope is so bad that you would not be able to see other things as well! Make sure that the microscope has a condenser. So it should not be too low grade.
Self diagnosis is highly problematic, as standardization required for proper comparison with reference values is difficult. Provided that microscopy is even capable of doing that.
>Also what magnification and things are needed to see platelets?
About 400x and staining necessary. en.wikipedia.org/wiki/Platelet
@@Microbehunter Not quite true, dear friend! How about sickle cell anemia, multitude of blood disorders, including other anemias, blood cancers and so many many other troublesome and even deadly diseases. Even today (2020) despite all the advances, haematologists rely on microscopy for diagnosing what brings them their bread and butter...! And pathologists as well!!
You're completely wrong about blood microscopy having no diagnostic value.
Blood films are one of the common haematological investigations in medicine.
en.wikipedia.org/wiki/Live_blood_analysis
He stated in a comment from a month ago he was referring specifically to this.
@@burz96 His original comment didn't though.
Calling live blood analysis or any other blood microscopy a nonsense is a very reckless statement.
Was this magnified to 1000x with those objective lens? Or digital zoom was used in this video through a phone?
😂😂😂 no answers
I have a question please help I have blood that the black blobs come and start eating the other cells lots of them I don’t understand what it is but I have the video I can send when I look in my microscope I didn’t see them but once I put my phone on the microscope which would be higher magnification you can see them going crazy on eating the cells can you please let me know thanks
Are u cv19 vaxxed?
When you say x4 objective, do you also have a x10 eyepiece, so the magnification is actually x40?
Yes, he certainly does for one 40x magnification is somewhat limited. It has the inadequate magnification to view red blood cells as clear and comprehensive as he did at, 1:33 where he used the 40x magnification objective. And for two, a compound microscope has two different lenses, the objective lens and the eyepiece. The average eyepiece is 10x, so I believe you would be correct in stating that 4x magnification was 40x. He also once declared in another video that he uses a 10x eyepiece which confirms your suspicions.
Microbe hunter , what microscope did you use to do all of that?
😂😂😂no answer.
It is possible to distinguish leucocites in polarized light?
Mistery of life and nature like a matrioska there is life in many dimensions
When do you use a stain with blood slides? And are you just using tap water to dilute. THanks!
Just water. Stains used for making white blood cells visible (especially the nucleus).
One scientist was slightly harmed in the making of this video.
What microscope would you recommend if you want to look at your blood
😂😂😂 no answers
What kind of microscope do I need to observe my blood LDL and HDL, and other types of lipids?
Lipids are molecules and too small to be seen with a microscope. You need to do a blood test. Doctors can do this by sending a blood sample to the lab.
I'm confused by the term 40x magnification. At 3:37 you say you're at 40x magnification but 40x .1mm is a lot smaller than the .1mm bar in the video. What am I missing?
40x objective, not 40x total magnification.
What do you think of "live blood analysis" that some naturopaths do. A scam? Or good info.
Can you please exlpain more about darkfield .
Which strain is used ??👀
1:41 Graph out the screen with 0,0 in the bottom left corner. in the upper left corner, approximately 2,8. You can see two blood cells traveling up(in relation to the video). Watch as one goes under a clear cell, it magnifies even more...............
I'm trying to decipher what the heck this comment means but I'm lost
What magnification is needed to see the indents in the red blood cells? Another 10X? 100X? This would be 6000X or 60000X total?
Recently I was able to see the indents using 600x brightfield microscopy. I was using 10x eyepieces with a 60x dry objective.
Question for you how do you look at virus under the microscope
You need an electron microscope, which I do not have. They are too small for light microscopes.
Rouleau is from not drinking enough water daily.
I have a microscope I purchased for my son almost a year ago sometime after his mother my wife passed. Trying to keep him active. Unfortunately is did not work. Time was the only solution. Anyways, I have this microscope by swift. And also a camera for it. But the camera has never been used and I cannot seem to get it to work. At any rate. You mentioned that observing the red blood cells is not a means of diagnosis. Is observing it over a period of time help in anyway to understand the changes in your body and diet? I thought about doing an experiment. For example, I drink soda and eat all the wrong things. Would looking at my blood now be any different 1, 2, 4, 6 months from now if I change my diet? Thank you for your feedback or anyone else's feedback that's familiar with this subject.
There might be changes in the blood, but these changes are not visible with a microscope. Eg. the blood fat levels might change, cholesterol levels, etc etc. All of these things can be determined by chemical measurements, but not with a microscope. As a matter of fact, these blood parameters are often tested when doing a general health check-up.
@@Microbehunter Thank You for the feedback. I actually look forward to using this microscope I have an seeing and learning about what the naked eye cannot see.
thankuuuuuuuuuuuuuuuuuuu
Why you called red blood cell although RBC do not possess any kind of nucleous?
They had nucleous before complete maduration. So, by convenience biologist or medical professionals call it cell or erythrocyte.
What microscope was used for this ?
😂😂😂 no answers
For Science..!! 😍😍😍
QUESTION?
what kind of water can i use to dilute blood as you dud on the glass slide?
Distilled is the most common I think..? Can i simply use bottled water (mountain spring water) .. i only want to dilute and examine under a scope.. living blood.. not a stained blood side (dry/dead?)..
Thank you.
This is an old video, so i dont not expect a response right away. 🤷♀️
If you add water, then the cells are going to pop due to osmosis. I did not use water. You have to streak out the blood to spread it.
@@Microbehunter in this particular video you did use a clear liquid where the blood was.. it did not mix together but it was a clear liquid @ 0:40 I’d like to try and replicate this..
@@Microbehunter thank you for you replay 👍🏼
phase contrast set guide?
Anemia falciforme sent a hug
:07 Aw man, you poor guy!! But happy times for us, opportunist that you are. :D
For 40x mag...which eye piece should i use? 10x?
He used 10x, although you can go higher or lower. It is your preference.
Would you please show us how to make a stained blood smear to make white blood cells stand out, and if you can, could you show us how to make the stained blood smear slide permanent? And what are the best stains and mountants to use? Is Haematoxylin stain useful for this? Thanks.
Watch this: th-cam.com/video/-4-sg8diBYE/w-d-xo.html
at 2:36 on wards what are the tiny white objects that move like daphnia ?
I don't know. it could be merely some dirt moving due to Brownian Motion.
may i ask you why live blood analysis has no diagnosis value? I have heard of this and since doctors do not help with my symptoms i wanted to get live blood analysis done and i heard it is extremely helpful for many.. but when i saw your video you said blood cells can't tell you anything like that.?
en.wikipedia.org/wiki/Live_blood_analysis
I have been to a live blood analysis and my blood cells was like double circles and they told me it show that my adrenals is working overtime and I need to use stress control drops. What is your opinion about this?
@@Microbehunter I went in sept last year and my life has changed for the better. Truth is in my experience Now I know I’ve been right all this time
I highly recommend live blood analysis
How if cupping blood see in microscop
2:33 what objective?
Blood cells aggregate when you drink unstructured water. As a result they cannot pass through vessels with very tiny diameter. This leads to atherosclerosis and other problems.
Just started watching your videos. Very interested in a good quality microscope that I can view images and video on my iMac. Willing to spend around a $1000. Which looking at your microscope might not be enough money. Lol. You tell me. Thanks
😂😂😂no answers
Glad I saw this and took the advice of adding water to the blood sample.
I was just looking into becoming a live blood analysis student why do you say don't ??? I want to detect lymes in the blood for people with a dark light microscope is this possible??
Both Live blood analysis and Chronic Lyme Disease (not to be confused with Lyme Disease) are two examples of health fraud and there have been court cases over this already:
en.wikipedia.org/wiki/Chronic_Lyme_disease
en.wikipedia.org/wiki/Live_blood_analysis
The Wikipedia articles cite the original sources, if you don't trust Wikipedia. There are molecular biological methods to diagnose Lyme disease, but microscopy is not one of them (concentration of bacteria too low). The things that live blood analysis finds are often artifacts that are interpreted as being something meaningful, which it is not. It is like palm-reading. By promoting such things, one can do more harm than good, because this way people are prevented from seeking genuine medical treatment. You asked me for my opinion, and I gave you my opinion. As microbiologist, I can only suggest that you seek training in a "proper" field, and if a full time study in medicine or biology is not possible, then I suggest some training to become a lab technician, rather than to spend resources on something that has no scientific basis. These things are sometimes used by alternative health practitioners, and people without scientific background are fooled into believing that there is something to it. If things go wrong in evidence-based medicine (the real medicine) then there is a system in place to correct this. If a treatment or diagnosis does not work, then it is thrown out. The problem with LBA and Chronic Lyme is that it has already been shown to be wrong and proponents still adhere to it. This is not a scientific approach. I know that my direct words might be a bit shocking, but you asked me for my opinion.
@@Microbehunter WOOOOOOOOOOOOOOOOOW thank you!!!!!!!!!!!!!!!!!!!!!!!!
i am 1st year college student at 41 i want to do microbiology in that feild how would you suggest i get into it? start as lab tech once i get my two year AA in science??? my fafsa covers me up to my 4 year degree. i am old comparing to others is there a future for me in this really?
@@Microbehunter This lyme blood diagnosis the way you know is not completly like that. There is a way to concentrate the borrelia in the sample and must be kept on 35 celsius. : DualDur technice
@@prettyparadoxicalwoman8285❤
2:53 ~ 3:20 I can see a few micro worms in blood. micro parasite ? I saw many worms in my blood yesterday.
Plot twist: planning another video for bandage under the microscope
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