Quantify bands with ImageJ | Western blot analysis on SDS-PAGE |Quantify band intensity with ImageJ

In this video we will see how densitometry is done for electrophoresis gel or SDS-PAGE or western blotting with the help of ImageJ software. ImageJ is very useful software and commonly used for the measurement of band intensity. You do not need to download any plugins or macros. Therefore, it is pretty much easy analysis.
This video lecture describes how to quantify western blot using ImageJ/ Fiji.
The steps involved are:
Step 1. How to make bands more visible
Step 2. How to invert image in ImageJ/ Fiji
Step 3. How to subtract background and adjust brightness and contrast of western blot / gel electrophoresis image in ImageJ/ Fiji
Step 4. How to rotate image in ImageJ/ Fiji to make the lanes in the straight line
Step 5. How to select the bands image to quantify
Step 6. How to plot lanes in ImageJ/ Fiji
Step 7. How to make the calculation for western blot after the quantification by calculating ratios and draw graphs.
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This is a preliminary guide undergraduate and postgraduate students and researchers/ scientists.
This video also gives an idea of:
• ImageJ tutorials
• Automated image processing
• ImageJ software
• Image analysis with ImageJ
• Image analysis with Fiji
• Spatial measurement with ImageJ
• Spatial measurement with Fiji
ImageJ is free software. It can be downloaded from:
imagej.nih.gov/ij/download.html
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How to calibrate ImageJ (Fiji) | Scale bar in ImageJ (Fiji) (Urdu/हिंदी)
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#imageJ #Fiji #densitometry #gelbandquantification #bandintensity #Westernblotquantification #SDS-PAGEmeasurement #immunoblotting #imageanalysis #Calibration
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[@mohammadansab8649]

Sir what is the purpose of subtracting background?