You explain things in a way that is so easy to understand and I love your visuals as well! Your channel has been a go-to for my microbiology class, thank you and keep up the great work ^.^
I just learned more from your video in 10 minute than what I was trying to understand in an hour lecture with my professor. Your awesome, it needs more professors like you.
Superb,really made understand the whole genetic recombination topic,i watched all 3 videos and feel that I'm confident for my final exam on Monday ,Thank you
8:46 so the phage DNA can be excised from the chromosomal DNA, where there it degrades the chrom DNA like in step 2 and that' s how the lytic cycle continues? Also you have the chrom DNA drawn as if its a nucleus but there is no nucleus right since its a prokaryote? is that circle just supposed to signify the compactness of the chrom dna?
How does the Phage perforate the Cell wall and Cell membrane of the bacteria to inject its dna inside the bacteria?In the Lysis cycle what makes the bacterial host cell to burst open?
I've been asked to make updated versions of my Transduction and Transformation videos, but I also don't want to "lose my place" in search results by replacing these older videos that have a lot of views already. If I were going to do another one, what would you want me to update or add to distinguish it from the original?
1- after the recombination of phage and bacterial DNA can the lytic cycle be resumed again ??? 2- are their possibility to formation transfer segment contain genes from three organisms (1st ,2ed bacteria and the phage ) ???
Cool video. I wish you would of mentioned the different types of transduction. For example, 1. generalized transduction and 2. specialized transduction.
Tottaly agree. I have to admit I was looking for a video that can explain me what you've just mentioned due to my misunderstanding these two types of transduction. Anyway, it is a really good video! Biology Professor, keep going!
though these question are not related with this video ,but being 'BIOLOGY PROFESSOR' I HOPE YOU WILL NOT MIND ANSWER THESE 2 QUESTIONS. 1-in some viruses the capsid is surrounded by a phospholipid membrane which is derived from the host cell HOW VIRUS CAN DERIVE PHOSPHOLIPID MEMBRANE FROM HOST CELL? 2-One small fungal thread is a hyphae. Many hyphae bundled together make mycelium then what is hypha?it is singular of hyphae so hyphae is plural but hyphae itself is ONE fungal thread how anything can be one and plural of something at the same time?i mean if one single thread is hyphae then what it's singular hypha is?
mam ,Do all bacteria have different genes?in transduction say foe eg.resistance from antibiotics is transfered from one bacteria to another bacteria? ,so why that bacteria did not have that gene for resistance from antibiotics earlier (so that it gained from another bacteria)whereas other bacteria (doner)have that gene?do all of bacteria have different genes?what if recipient already have that gene it will not take it from other bacteria?
Bacteria of different species have different sets of genes. This is why bacteria like those that cause tetanus, botulism, anthrax, strep throat, scarlet fever, ulcers, etc. are so different from each other and also so different from many of the good symbiotic bacteria in our bodies. Genes can be transferred between different species or different strains of the same species through transduction, as shown in this video, or through transformation (th-cam.com/video/dKD19cXkWBw/w-d-xo.html) or conjugation (th-cam.com/video/YycVGqBs1p0/w-d-xo.html). Bacteria replicate through binary fission (th-cam.com/video/ckAHRAC48nY/w-d-xo.html) and receive an initial set of genes from the parental cell. Sometimes this includes antibiotic resistance genes (th-cam.com/video/yU258z8iftA/w-d-xo.html) or special virulence factors. If not, these genes can be acquired through one of these other mechanisms. It is also possible for some bacteria to gain multiple copies of a gene, but I don't think this is very common.
Those are some good questions. The speed of the bacteria cell's destruction by the phage is dependent on several factors, including the type of phage, how long it takes to replicate, the type of bacterium, and whether the phage is in the lytic or lysogenic cycle. Destruction of the host cell in the lytic cycle is much quicker than in the lysogenic cycle and probably only takes a matter of minutes (I think), but is dependent on those other factors I mentioned. Some phages cannot successfully infect certain types of bacteria because of various host defenses. For example, some bacteria produce restriction enzymes that degrade phage DNA in order to prevent phage replication. Phages also generally enter bacterial host cells by binding to receptors on the cell surface, meaning they can't infect bacteria that lack those receptors.
Biology Professor thank you, in my case there is hemolytic E.coli. I hope phages will kill it fast. One more question, can phages survive in acid environment of human stomach to go to intestines?
Baatr Mandzhiev That is really outside my specialty. According to this article (www.pnas.org/content/109/43/17621.full.pdf), phage can live in the intestines. But I assume this probably isn't true for all types of phage. If you are receiving phage therapy for an E. coli infection, your question is best directed to your doctor who will know the specifics of your case. Sorry I can't help more!
thankyou for this wonderful video and easy explanation i got a perfect score for my test after i watched this video. i am requesting for videos about protists, the mold protists, plants protists and animal protists thankyou :))))
Sort of. In the lysogenic cycle, the virus inserts its DNA (called a prophage) into the bacteria cell, which continues to grow and divide. If the viral DNA is carrying a gene from a previous bacteria cell, that would also be inserted into the new bacteria cell's DNA. It is possible to design a virus that carries a specific gene, so I suppose theoretically it is possible to infect cells with that designed virus carrying your gene of interest. But that would only be possible in laboratory conditions. In natural conditions, you cannot control which bacteria DNA a virus picks up and transfers to a new cell. For more information on the lysogenic cycle, see this link: en.wikipedia.org/wiki/Lysogenic_cycle
Hi Nedariya! I have added a new video about Davis' U-Tube experment based on your request. Thanks for suggesting this as a topic! Check out the video here: th-cam.com/video/YEXMuB4gsu4/w-d-xo.html
Mr. Jones, You are correct that there is a large difference in plasmids and chromosomes. However, you are incorrect in asserting that transduction may only involve plasmid DNA. In fact, transduction primarily results in the transfer of chromosomal DNA. Here is a quote regarding this topic from "An Introduction to Genetic Analysis" (7th edition) by Griffiths et al.: "There are two kinds of transduction: generalized and specialized. Generalized transducing phages can carry any part of the chromosome, whereas specialized transducing phages carry only restricted parts of the bacterial chromosome." I invite you to read more about this topic at: www.ncbi.nlm.nih.gov/books/NBK21760/ (the National Center for Biotechnology Information website). The fact that transduction specifically refers to the transfer of bacterial chromosomal DNA by viruses is also upheld in the 9th edition of "Microbiology: An Introduction" by Tortora, Funke, and Case (pp. 244-245), a commonly used microbiology textbook at many universities. On the other hand, transfer of plasmids between bacteria occurs through a process known as conjugation. If you want, you may view my video on conjugation here: th-cam.com/video/YycVGqBs1p0/w-d-xo.html Best, Biology Professor (I have a Ph.D. in Microbiology & Immunology)
Shehzad Ahmed Idiot, You're here to study so stop embarrassing yourself and others, Respect your Professor, In my Culture a person who conveys knowledge is no less than God, So we respect people who transfer knowledge.
This was such a well done video. the audio quality wasn't the best but the explanation was tip top!
I love the way you're all serious throughout the lecture and at the very end flash that big smile when your done :p
You explain things in a way that is so easy to understand and I love your visuals as well! Your channel has been a go-to for my microbiology class, thank you and keep up the great work ^.^
Thank you! I am not an artist, so I appreciate the compliment on my visuals. :) Good luck in class!
Thank you, this is the most helpful video on this topic that I've found on TH-cam. Great teacher.
Wow - thanks! :)
Can l communicate with you
your explanation is so good but the sound is too noisy and I can't hear you very well
I hope you will fix the sound
I just learned more from your video in 10 minute than what I was trying to understand in an hour lecture with my professor. Your awesome, it needs more professors like you.
You are so good an explaining this topic!! So easy to understand!
you just have no idea how much you helped me thank youuuu
you are the best professor ever ^^
These videos are so helpful and you are so good at explaining these concepts. Thank you!!! P.S. You are absolutely drop dead gorgeous!
Superb,really made understand the whole genetic recombination topic,i watched all 3 videos and feel that I'm confident for my final exam on Monday ,Thank you
Love from India🇮🇳🇮🇳....
8:46 so the phage DNA can be excised from the chromosomal DNA, where there it degrades the chrom DNA like in step 2 and that' s how the lytic cycle continues? Also you have the chrom DNA drawn as if its a nucleus but there is no nucleus right since its a prokaryote? is that circle just supposed to signify the compactness of the chrom dna?
How does the Phage perforate the Cell wall and Cell membrane of the bacteria to inject its dna inside the bacteria?In the Lysis cycle what makes the bacterial host cell to burst open?
Thanks a lot. This video made me to understand this topic better. Really appreciate it.
Great video, biology professor! Could you make an updated version of this video?
I've been asked to make updated versions of my Transduction and Transformation videos, but I also don't want to "lose my place" in search results by replacing these older videos that have a lot of views already. If I were going to do another one, what would you want me to update or add to distinguish it from the original?
1- after the recombination of phage and bacterial DNA can the lytic cycle be resumed again ???
2- are their possibility to formation transfer segment contain genes from three organisms (1st ,2ed bacteria and the phage ) ???
1. Yes
2. Theoretically, yes, this could happen
Mercii beaucoup. Il n y a pas de video en francais comme celle ci donc la votre m a beaucoup aider ^^
Vous êtes les bienvenus! Bonne chance d'étudier!
You are very welcome! Good luck studying!
You seem to have a video on every topic in microbiology
Yes, I’m running out of ideas ;)
Really helpful:)
Thank yew so much for existing
Cool video. I wish you would of mentioned the different types of transduction. For example, 1. generalized transduction and 2. specialized transduction.
Tottaly agree. I have to admit I was looking for a video that can explain me what you've just mentioned due to my misunderstanding these two types of transduction. Anyway, it is a really good video! Biology Professor, keep going!
SUCH A GREAT VIDEO!
Thanks 😊
You're saving me in college genetics
You are welcome. Glad to help!
Thank you, very useful video
thank you for a good explanation! very helpful
though these question are not related with this video ,but being 'BIOLOGY PROFESSOR' I HOPE YOU WILL NOT MIND ANSWER THESE 2 QUESTIONS.
1-in some viruses the capsid is surrounded by a phospholipid membrane which is derived from the host cell HOW VIRUS CAN DERIVE PHOSPHOLIPID MEMBRANE FROM HOST CELL?
2-One small fungal thread is a hyphae. Many hyphae bundled together make mycelium then what is hypha?it is singular of hyphae so hyphae is plural but hyphae itself is ONE fungal thread how anything can be one and plural of something at the same time?i mean if one single thread is hyphae then what it's singular hypha is?
Very helpful
mam ,Do all bacteria have different genes?in transduction say foe eg.resistance from antibiotics is transfered from one bacteria to another bacteria?
,so why that bacteria did not have that gene for resistance from antibiotics earlier (so that it gained from another bacteria)whereas other bacteria (doner)have that gene?do all of bacteria have different genes?what if recipient already have that gene it will not take it from other bacteria?
Bacteria of different species have different sets of genes. This is why bacteria like those that cause tetanus, botulism, anthrax, strep throat, scarlet fever, ulcers, etc. are so different from each other and also so different from many of the good symbiotic bacteria in our bodies. Genes can be transferred between different species or different strains of the same species through transduction, as shown in this video, or through transformation (th-cam.com/video/dKD19cXkWBw/w-d-xo.html) or conjugation (th-cam.com/video/YycVGqBs1p0/w-d-xo.html). Bacteria replicate through binary fission (th-cam.com/video/ckAHRAC48nY/w-d-xo.html) and receive an initial set of genes from the parental cell. Sometimes this includes antibiotic resistance genes (th-cam.com/video/yU258z8iftA/w-d-xo.html) or special virulence factors. If not, these genes can be acquired through one of these other mechanisms. It is also possible for some bacteria to gain multiple copies of a gene, but I don't think this is very common.
thanks for your answer
What is the speed of getting a bacteria destroyed? And why some phages still skip the bacteria they are supposed to eat?
Those are some good questions. The speed of the bacteria cell's destruction by the phage is dependent on several factors, including the type of phage, how long it takes to replicate, the type of bacterium, and whether the phage is in the lytic or lysogenic cycle. Destruction of the host cell in the lytic cycle is much quicker than in the lysogenic cycle and probably only takes a matter of minutes (I think), but is dependent on those other factors I mentioned. Some phages cannot successfully infect certain types of bacteria because of various host defenses. For example, some bacteria produce restriction enzymes that degrade phage DNA in order to prevent phage replication. Phages also generally enter bacterial host cells by binding to receptors on the cell surface, meaning they can't infect bacteria that lack those receptors.
Biology Professor thank you, in my case there is hemolytic E.coli. I hope phages will kill it fast. One more question, can phages survive in acid environment of human stomach to go to intestines?
Baatr Mandzhiev
That is really outside my specialty. According to this article (www.pnas.org/content/109/43/17621.full.pdf), phage can live in the intestines. But I assume this probably isn't true for all types of phage. If you are receiving phage therapy for an E. coli infection, your question is best directed to your doctor who will know the specifics of your case. Sorry I can't help more!
Thank you!!
You are welcome!
precisely what I needed thank you
june 1st gang?
Very poor audio quality
Really helpful mam.. 👌
thanks
you're a good master
Subscribed after 3 seconds
subscribed after 1 second.
thank u so much mam
thankyou for this wonderful video and easy explanation i got a perfect score for my test after i watched this video. i am requesting for videos about protists, the mold protists, plants protists and animal protists
thankyou :))))
my requests meant like tje life cycle of oomycota, acrasiomycota life cycle, myxomycota life cycle and so on
the same issue you need to find a solution to the voice
very good and professional explanation
by using the color I got the point hhhhhh thanks
can a virus be made to transfer a specific gene to a bacteria without killing the bacteria?
Sort of. In the lysogenic cycle, the virus inserts its DNA (called a prophage) into the bacteria cell, which continues to grow and divide. If the viral DNA is carrying a gene from a previous bacteria cell, that would also be inserted into the new bacteria cell's DNA. It is possible to design a virus that carries a specific gene, so I suppose theoretically it is possible to infect cells with that designed virus carrying your gene of interest. But that would only be possible in laboratory conditions. In natural conditions, you cannot control which bacteria DNA a virus picks up and transfers to a new cell. For more information on the lysogenic cycle, see this link: en.wikipedia.org/wiki/Lysogenic_cycle
hello mem something say about u tube experiment by Barnard davis
This is a really cool experiment and I have added it to my list of videos to prepare. Thanks for the suggestion!
Hi Nedariya! I have added a new video about Davis' U-Tube experment based on your request. Thanks for suggesting this as a topic! Check out the video here: th-cam.com/video/YEXMuB4gsu4/w-d-xo.html
thiz vedio so helpfull ...thank u fr thz
can i ask what COTRANSDUCTON is?
The simultaneous transduction of multiple genes, like two or more marker genes.
4:19 the bacterial chromosome is...?
The bacterial chromosome is degraded. Sorry about the sound, that is one of my earliest videos and I didn't have as good of a microphone back then.
@@BiologyProfessor thanks alot
Mam send a lecture on molecular biology of light reaction
I suggest you watch this video instead: th-cam.com/video/GR2GA7chA_c/w-d-xo.html
Ty ma'am
I wish you Come to our university and teach us microbiology 😄❤
Oh thanks thanks thanks ❤❤
Thankyou
Nice explaination but voice is not clear
Mam aap bahut achha parati hai but mam please ye video hindi me aapload kiziye
It is not chromosomal DNA, it is plasmid DNA. There is a large difference between plasmids and chromosomes.
Mr. Jones, You are correct that there is a large difference in plasmids and chromosomes. However, you are incorrect in asserting that transduction may only involve plasmid DNA. In fact, transduction primarily results in the transfer of chromosomal DNA. Here is a quote regarding this topic from "An Introduction to Genetic Analysis" (7th edition) by Griffiths et al.:
"There are two kinds of transduction: generalized and specialized. Generalized transducing phages can carry any part of the chromosome, whereas specialized transducing phages carry only restricted parts of the bacterial chromosome."
I invite you to read more about this topic at: www.ncbi.nlm.nih.gov/books/NBK21760/ (the National Center for Biotechnology Information website).
The fact that transduction specifically refers to the transfer of bacterial chromosomal DNA by viruses is also upheld in the 9th edition of "Microbiology: An Introduction" by Tortora, Funke, and Case (pp. 244-245), a commonly used microbiology textbook at many universities.
On the other hand, transfer of plasmids between bacteria occurs through a process known as conjugation. If you want, you may view my video on conjugation here: th-cam.com/video/YycVGqBs1p0/w-d-xo.html
Best,
Biology Professor
(I have a Ph.D. in Microbiology & Immunology)
Sounds are not good jeez
👍
noiceeeeee
Isn't she cute
pls improve audio its verrrrrrrrrrrrrrrrrry irritating....
This is an old video. I can’t go back in time to fix it. Sorry. 🤷🏻♀️
@@BiologyProfessor remake if possible
she cute tho
figure is awesome
I assume you mean the diagram on the board ;)
Shehzad Ahmed Idiot, You're here to study so stop embarrassing yourself and others, Respect your Professor, In my Culture a person who conveys knowledge is no less than God, So we respect people who transfer knowledge.
😂 Hr jaga bure log hote hy
I didn't mean anything negative to the teacher but, I was praising her and if praising someone is a crime then I'm the criminal.
Professor I apologise.