Approximation is inherently flawed if your goal is to get a correct answer. However, it can be a very good guide in many instances. The dataset I show here intentionally creates a situation where the approximation is misleading because the dataset is not complete - the measured velocity does not approach Vmax. Consequently it is not possible to accurately estimate Vmax, which means the Km estimate is also flawed. Conversely, the Lineweaver-Burk approach also has some inherent issues that I don't discuss in this video which can result in incorrect Km and Vmax values.
These would come from individual experiments where you are monitoring the rate of the reaction. For example, if you set up an experiment that monitors the absorbance of a product and plot it vs. time, the slope of that line would be related to the rate - if this is done under controlled conditions, that would be your V0
Please show how this is done manually without excel
Does this mean that the approximation method is very wrong? Because the answers for Km and V max are very different
Approximation is inherently flawed if your goal is to get a correct answer. However, it can be a very good guide in many instances. The dataset I show here intentionally creates a situation where the approximation is misleading because the dataset is not complete - the measured velocity does not approach Vmax. Consequently it is not possible to accurately estimate Vmax, which means the Km estimate is also flawed. Conversely, the Lineweaver-Burk approach also has some inherent issues that I don't discuss in this video which can result in incorrect Km and Vmax values.
with using a Bio Rad plate we obtain a number that is absorbance how we can convert the Vo in concentration/time ?
In this video I can't really figure out the calculation of the y intercept from the graph.
Thankyouu,
How did you calculate Vo ?
These would come from individual experiments where you are monitoring the rate of the reaction. For example, if you set up an experiment that monitors the absorbance of a product and plot it vs. time, the slope of that line would be related to the rate - if this is done under controlled conditions, that would be your V0
Thank you, it helped so much now haha
Perché b=0.004?