Transformation Vs Transfection
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- เผยแพร่เมื่อ 29 ส.ค. 2024
- This lecture explains about the differences between transformation and transfection. The three very effective modes of gene transfer Transformation, Transduction and Transfection observed in bacteria fascinated the scientist leading to the development of molecular cloning. The basic principle applied in molecular cloning is transfer of desired gene from donor to a selected recipient for various applications in the field of medicine, research, gene therapy with an ultimate aim of beneficial to the mankind.
Transformation: Transformation is the naturally occurring process of gene transfer which involves absorption of the genetic material by a cell through cell membrane causing the fusion of the foreign DNA with the native DNA resulting in the genetic expression of the received DNA. Transformation is usually a natural method of gene transfer but as a result of technological advancement originated the artificial or induced transformation. Thus there are two types called as natural transformation and artificial or induced transformation. In natural transformation, the foreign DNA attaches itself to the host cell DNA receptor and with the help of the protein DNA translocase it enters the host cell. The presence of nucleases restricts the entry of two strands of the DNA, destroys a single strand thus allowing only one strand to enter the host cell. This single stranded DNA mingles with the host genetic material successfully.
The artificial or induced method of transformation is done under laboratory condition which is either a chemical mediated gene transfer or done by electroporation. In the chemical mediated gene transfer, the cold conditioned cells in calcium chloride solution are exposed to sudden heat which increases the permeability of the cell membrane allowing the foreign DNA. The electroporation method as the name indicates, pores are made in the cell by exposing it to suitable electric field, allowing the entry of the DNA. The opened up portions of the cell are sealed by the ability of the cell to repair. Transfection: One of the methods of gene transfer where the genetic material is deliberately introduced into the animal cell in view of studying various functions of proteins and the gene. This mode of gene transfer involves creation of pores on the cell membrane enabling the cell to receive the foreign genetic material. The significance of creating pores and introducing the DNA into the host mammalian cell contributed to different methods in transfection. Chemical mediated transfection involves use of either calcium phosphate or cationic polymers or liposomes. Electroporation, sonoporation, impalefection, optical transfection, hydro dynamic delivery are some of the non chemical based gene transfer. Particle based transfection uses gene gun technique where a nanoparticle is used to transfer the DNA to host cell or by another method called as magnetofection. Nucleofection and use of heat shock are the other evolved methods for successful transfection.
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Sir your method to teaching is very good....I regularly watched your video tutorial...thanx for giving a such information 👌👏
How the hell do you have a video for literally every topic
I do make them 365 days a year
I think the ideas of your vedios are good for getting the foundation of the concepts. However, one should know that there processes are much complex than that. Eg, bacterial cells transformation requires 2 steps, they need to be made competent and a heat shock is needed to open up the bacteria allowing acquisition of DNA. Over all., the vedio are good for person who wants to get the idea. But further study is required. Thank you sir , your effort is apreciated.
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Great sir
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audio could be betr, but i guess it was the 00's so the technology wasnt thr yet. gr8 vid. _JC
Gooood
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Sir aap kahn the aabtk ..
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Really liked ur way...
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very smart guy , i appreciate your video a lot
Ultimately explain
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besssttttt explainations for life science r here
+chhavi saxena thank you. Glad you liked my lectures
Acording to you these two terms are used as transfection for eukaryotic cel and transformation for prokaryotic cell. Than why wo use the word transformation in case of plant cel i.e. Agrobacterium mediated plant transformation, PEG mediated Plant transformation, Bialistic transformation?
Bro small correction transfer the gene/genetic by using virus is called transduction. Whereas transfer the genetic material by non viral methods either physical or chemical is called transfection
Actually the word of tranfection is the combination of two words 1) transformation and 2) infection so the trans from the 1 and the fection from the 2 combine to form transfection
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This video was helpful Sir
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Very good
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You explained it so nicely.... Thnku
+Kiran Chauhan thank you. Glad you liked my lectures
In the case of Transformation, even if it intakes ssDNA it will eventually be degraded inside the cell. Usually, circular plasmids are being transformed.
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@@shomusbiologyofficial Sir today in my 4th year {Genetic engineering+Biotech } exam 🇧🇩 and a question was What is "Transfection". Yesterday night i watched ur video and it benefit me 😃 ❤️🤝
Ur awesome sir thank u for ur videos
U said about plasmid putting in eukaryotes... Can it replicate the same way as it replicates in prokaryotes
Please make a video of direct and indirect methods of gene transfer
Well explained sir. Tysm❤️💯
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But exactly what make eukaryotic cell difficult to take dna ?
The cell wall and nuclear envolope that protects the nucleas.
Nice 👍
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Explain the process nd why it's need for cell nd whr it used
Is it necessary nd why
DNA receptor on the surface of the bacteria!!
Alan Byron yes sir what is it . actually I don't know any dna receptor on cell membrane that's always wrong.
Sir what is exactly the types of transformation in molecular biology?
tx sir
You're welcome
Is transfection involve vector mediated
Eukaryotes ka liya kaise palsmid vector ho sakta ha ?
sir , what are the causes of transformation
Sir plz explain sonoporation quickly
Ek Vedio calcium phosphate precipitation pe bhi Dalo sir plz plz plz
Okay
transformation is always wrong because there is no cacl2 treatment on cell membrane there is no heat shock .so please give a correct information about this transformation
*3:00** a lipid nano particle which is proprietary!* could it work... (no turns out. real money maker tho.) _JC
Can u please send this man to my college ?😢😢😢
No one will do that.
So why in plant cell we called it transformation?
Because in animal cells, transformation means cancer formation
There goes an hour and half lecture
superbbbb..
Sir u r too much cute 😀
What you are talking about is transduction not transfection😇
Pls hindi mein smjhaya kre
Contain many misinformations.
Kindly urdu m bola kry
Ok
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