Hey, heres my anti-thesis, It sure looks safer, but to me the many disadvantages ( time needed between transfer, wiping the blade, having alcohol that will transfer to the agar plates,making a aseptic alcool soup, not having certainty about the sterilization of the blade) weight out against the only advantage which is maintaining sharpness of the blade. And is that whole process really saving money? Scalpel blades are 10ct€ piece in a 100pack, how much alcohol does this consume to save one blade? If one just want to avoid fire hazard i recommend making a DIY induction induction coil sterilizer. This tek goes against all i have learnt (alcool sanitize, heat sterilize). Cheers
Hey Linus. This tek works. I had no contamination issues with it. Here is how I apply the process: 1. The knife is in the closed storage tube, placed in ethanol. 2. When I need it, I put the knife holder into the still air box. 3. There I take it with the magnet out of the storage tube and place it into the shorter working tube. The ethanol mainly stays in the storage tube and only little bit drips on the ground of the working tube. The knife is clean, sterile and good to use. 4. When I cut several agar plates I sometimes have to use both hands. With the working tube I have a defined place for my knife. Whenever I do not need it, I put it there. (I do not put it back in to the ethanol filled storage tube.) Having a defined working place for the knife standardized the process and leads to lower contamination. 5. After finishing the cloning, I clean the working area and the knife. The clean knife is put back into the storage tube with ethanol. The tube is closed. I did share this tek because it works, and I wanted to give and second approach to the one that is mainly used and I also show in my other videos: alcohol sanitize, and heat sterilize. One could of course also use an induction coil heating system or just sterilize the blades at 3 bar and 134°C. But these second two approaches are more expensive to realize. I also wanted to raise awareness about the potential fire hazard that exists during work. I do not think that many people have fire extinguishers in their working area just to be prepared and hopefully never need to use them. If you ever work in a big production plant, you will see that there are many safety measures applied to prevent or minimize any possible bad outcome that could harm a person or the production process. You are right, the blades are cheap, and the fire hazard is also controllable. That means, if you are happy with the way you are working, just continue that way, nothing wrong with it! I find that having a defined place for putting my knife during work and just using it directly without extra heating the blade simplified the process and made mushrooming more fun for me.
@@diy-mushroom-tek @diy-mushroom-tek Hey thank you for the in depth explanation the video was great also, i did not knew why isopropyl was considered best before. Im too stubborn and old fashioned, even tho i believe that the tek works flawlessly, i will follow the herd on this one. I applaud your drive to experiment with novel techniques. And yeah peace of mind really is a value i can get behind, each one their priorities. As i am a begginer i want to start by following fool proof methods.
Thank you. I am absolutely shocked when I see people using fire and alcohol together. Why are they doing this and teaching this dangerous method? There are so many other safe ways.
Hi, good video and very interesting explanation. I have a question about the material you use to prototype the piece. What material do you use to do 3D printing? (Sorry if you mention it at some point in the video, it's because when the video is automatically translated, that information doesn't appear.) I read an article some time ago that certain prototyping materials are more likely to contain microorganisms than others due to their composition, roughness, etc. Well, great video.
Hey Miguel! Thank you for your kind words. I'm really glad to hear that you find value in my content! Regarding your question: I use the cheapest PLA for all my printing needs, which I usually purchase on eBay for about €10 per kilo. I typically use white for prototypes because it doesn’t absorb light, making the contours more visible and giving the model an aesthetic appeal. Additionally, with white, it’s immediately clear which parts are printed and which are not. For practical applications, I prefer black PLA. It stays cleaner for longer, especially in humid environments where white can easily show signs of insufficient air circulation. Black also blocks more light, so I use it for the humidifiers in my plant grow tents. Both colors have proven to be very durable and resilient against water in my experience. However, they don’t handle shock loads well, but since I use them primarily for structural components, this isn’t usually a concern for me. Happy Growing!
Hey, heres my anti-thesis,
It sure looks safer, but to me the many disadvantages ( time needed between transfer, wiping the blade, having alcohol that will transfer to the agar plates,making a aseptic alcool soup, not having certainty about the sterilization of the blade) weight out against the only advantage which is maintaining sharpness of the blade.
And is that whole process really saving money? Scalpel blades are 10ct€ piece in a 100pack, how much alcohol does this consume to save one blade?
If one just want to avoid fire hazard i recommend making a DIY induction induction coil sterilizer.
This tek goes against all i have learnt (alcool sanitize, heat sterilize).
Cheers
Hey Linus. This tek works. I had no contamination issues with it. Here is how I apply the process:
1. The knife is in the closed storage tube, placed in ethanol.
2. When I need it, I put the knife holder into the still air box.
3. There I take it with the magnet out of the storage tube and place it into the shorter working tube. The ethanol mainly stays in the storage tube and only little bit drips on the ground of the working tube. The knife is clean, sterile and good to use.
4. When I cut several agar plates I sometimes have to use both hands. With the working tube I have a defined place for my knife. Whenever I do not need it, I put it there. (I do not put it back in to the ethanol filled storage tube.) Having a defined working place for the knife standardized the process and leads to lower contamination.
5. After finishing the cloning, I clean the working area and the knife. The clean knife is put back into the storage tube with ethanol. The tube is closed.
I did share this tek because it works, and I wanted to give and second approach to the one that is mainly used and I also show in my other videos: alcohol sanitize, and heat sterilize.
One could of course also use an induction coil heating system or just sterilize the blades at 3 bar and 134°C. But these second two approaches are more expensive to realize.
I also wanted to raise awareness about the potential fire hazard that exists during work. I do not think that many people have fire extinguishers in their working area just to be prepared and hopefully never need to use them. If you ever work in a big production plant, you will see that there are many safety measures applied to prevent or minimize any possible bad outcome that could harm a person or the production process.
You are right, the blades are cheap, and the fire hazard is also controllable. That means, if you are happy with the way you are working, just continue that way, nothing wrong with it!
I find that having a defined place for putting my knife during work and just using it directly without extra heating the blade simplified the process and made mushrooming more fun for me.
@@diy-mushroom-tek @diy-mushroom-tek Hey thank you for the in depth explanation the video was great also, i did not knew why isopropyl was considered best before.
Im too stubborn and old fashioned, even tho i believe that the tek works flawlessly, i will follow the herd on this one.
I applaud your drive to experiment with novel techniques.
And yeah peace of mind really is a value i can get behind, each one their priorities.
As i am a begginer i want to start by following fool proof methods.
Hey Linus, thank you for your feedback. I value your thoughts and our discussions!
Thank you. I am absolutely shocked when I see people using fire and alcohol together. Why are they doing this and teaching this dangerous method? There are so many other safe ways.
Hi, good video and very interesting explanation. I have a question about the material you use to prototype the piece. What material do you use to do 3D printing? (Sorry if you mention it at some point in the video, it's because when the video is automatically translated, that information doesn't appear.) I read an article some time ago that certain prototyping materials are more likely to contain microorganisms than others due to their composition, roughness, etc. Well, great video.
Hey Miguel!
Thank you for your kind words. I'm really glad to hear that you find value in my content!
Regarding your question: I use the cheapest PLA for all my printing needs, which I usually purchase on eBay for about €10 per kilo. I typically use white for prototypes because it doesn’t absorb light, making the contours more visible and giving the model an aesthetic appeal. Additionally, with white, it’s immediately clear which parts are printed and which are not.
For practical applications, I prefer black PLA. It stays cleaner for longer, especially in humid environments where white can easily show signs of insufficient air circulation. Black also blocks more light, so I use it for the humidifiers in my plant grow tents. Both colors have proven to be very durable and resilient against water in my experience. However, they don’t handle shock loads well, but since I use them primarily for structural components, this isn’t usually a concern for me.
Happy Growing!