Polytene Chromosomes from Salivary glands | Dissection of Chironomus larvae

Giant chromosome - Temporary slide preparation of Polytene Chromosomes from Salivary glands of Chironomus larvae. Video explains the complete procedure of microscope slide preparation to perform practical in the laboratory. preparing a wet mount slide.
For detailed information visit: www.ebiologyla...
Polytene #Chromosome from Salivary glands of Chironomus larvae :
Materials required:
i) 4th instar Drosophila larvae
ii) Two pointed needles
iii) Microscope slide and coverslip
iv) Dissecting microscope
v) Aceto-alcohol (1:1) fixative
vi) Aceto-orcein stain
vii) 45% acetic acid
viii) Compound microscope
ix) Large watch glass or petri dish
x) Blotting papers
xi) 1N HCl
Procedure:
A) Dissection of Chironomus larvae:
1) Dissect the larva with the help of pointed needles as shown in the video.
2) Hold the posterior end of larva with one needle and pull the anterior part with the second needle.
3) Locate the salivary glands and remove other debris.
B) Fixation and staining of Salivary glands of Chironomus larva:
1) Fix the salivary glands with aceto-alcohol fixative for 5 minutes.
2) Remove the excess fixative with blotting paper.
3) Add two drops of 1N HCl over the salivary glands of Chironomus larva to hydrolyze them.
4) Incubate the slide for two minutes at 57 degree Celsius.
5) Remove the excess HCl with blotting paper.
3) Add two to three drops of Aceto-orcein stain over the salivary glands of Chironomus larvae.
4) Cover the slide with a petri dish or large watch glass and wait for 10 minutes.
5) After 10 min. takeout excess stain by washing the slide with 45% acetic acid.
6) Remove the excess stain and acetic acid with blotting paper.
7) Add one drop of Aceto-orcein stain over the salivary glands of Chironomus larvae.
8) Cover it with a coverslip.
C) Squash preparation of salivary glands of Chironomus larvae:
1) To squash, the salivary glands under coverslip place the slide between the fold of blotting papers and apply pressure with the thumb over the coverslip and roll it.
2) seal the edges of coverslip with nail polish or DPX.
D) Observation of squashed salivary glands of Chironomus larvae under a microscope for polytene chromosome.
#POLYTENECHROMOSOME (Giant chromosome) from #SALIVARYGLAND of Chironomus larvae also know as bloodworm (Chironomidae).
this video ( Polytene Chromosomes from Salivary glands) explain complete procedure to perform practical in the laboratory. one can use Drosophila larvae to perform this experiment. Also, we have demonstrated the procedure of salivary glands dissection or harvesting of salivary glands from Chironomus Larvae or Drosophila larvae.
Preparation of Polytene Chromosome from salivary glands of Chironomus Larvae.
E.G.Balbiani (1881) first reported the Polytene chromosomes.
Balbiani rings are exceptionally large puffs on the polytene chromosomes in the dipteran Chironomus salivary glands.

[@NEET2912neet]

I also done it in my lab..and it was very difficult for me to separate the salivary gland of larva..Your work and handling everything is in good and clean way..Thanku for sharing

[@DKBiosolutions]

Hello Anuradha...
I aslo found it difficult before practicing... Believe me at one point you will find it very easy...
Best luck for your practical... 😊👍

[@NEET2912neet]

@@DKBiosolutions Thanku

[@rajatar9568]

Your lab skills are wonderful we tried multiple times but no body was able to get the result I have exam tomorrow hope I get it
Thanks for this wonderful explaination!

[@DKBiosolutions]

I hope it helped you... Thank you 🙏😊

[@Anuradha_567]

Soo clean and Amazingly performed..👍👍

[@DKBiosolutions]

Thank you Anu.... 😊👍

[@labade42]

Wonderful video!
Precisely made chemicals and Skilful hands

[@DKBiosolutions]

Thank you sir 😊

[@Labtechnician_eraa]

Hello, this video is shown by our teacher Wasim sir in our practical before performing practical and it's really helped us👌👌👌

[@DKBiosolutions]

Wasim sir from Poona college???

[@DKBiosolutions]

I am glad to know that it helped you all😊👍

[@uzmashaikh8223]

@@DKBiosolutions Yes from Poona College 😊

[@uzmashaikh8223]

@@DKBiosolutions Thank you Sir this video was helpful for us and very well explained 👍👍

[@DKBiosolutions]

Thank you for appreciation 😊👍

[@Maitri24]

Very useful helpful thanks Sir 🙏👍👍 keep uploading new practical video

[@DKBiosolutions]

Yes... thank you so much for the support 🙏

[@shrikantmali8321]

Dipak Sir
Your techniques are really useful.
Thank you Sir

[@tanvirpathan2129]

You always give unknown information in nice way..

[@DKBiosolutions]

Thank you so much...

[@sw-ot9xj]

Very neat and clean perfect practical handling,👍👍

[@DKBiosolutions]

Thank you 😊

[@thesunilbutiya]

Nice practical video
Sir

[@lying_frog]

i have exam in 1st october.
😢😢😢😢 thank you for your video

[@DKBiosolutions]

All the best

[@krishnakanths4127]

I was excited to see this very thankful

[@DKBiosolutions]

My pleasure 😊👍

[@phidarihunt.makdoh]

A very helpful video... Thanks for uploading Sir 🙏

[@DKBiosolutions]

You are welcome 😊

[@papinandi4975]

It is a very helpful video.. Thank you sooo much

[@DKBiosolutions]

My pleasure 😊👍

[@akshaydabhade7862]

Ty sir .u can make study too easy

[@priyankachaure1348]

Knowledgeable vedio sir thanks for you sir totally clearance vedio

[@DKBiosolutions]

My pleasure 😊... I'm glad to know that it helped you...

[@nehamukherjee2142]

wonderful video ❤

[@DKBiosolutions]

Thank you ☺️👍

[@biotalksolution]

I am seeing just ur first video n only 4 min 46sec, i am going to subscribe u....well capture...very helpful...please upload more more and more videos of practical...spl thanks

[@DKBiosolutions]

Thank you so much for your appreciation 😊... From last 3-4 months I haven't done anything for TH-cam videos because I am about to submit my PhD thesis. In next 2 months I will be completely free. 😊👍

[@bhavikachhablani8146]

Sir can you give some tips for drosophila larva

[@DKBiosolutions]

Practice is the only key to get salivary glands in first attempt...

[@poojachdhry23]

excellent 😊

[@DKBiosolutions]

Thank you ma'am 😊🙏

[@msg3133]

Best video bro 🤗🤗

[@DKBiosolutions]

Thank you 😊👍...

[@TayyabaBasharat-u1w]

Very nice ❤

[@DKBiosolutions]

Thank you 😊

[@raiseyouremaan801]

Excellent

[@DKBiosolutions]

Thank you 😊

[@jasmithamonis8568]

superb

[@DKBiosolutions]

Thank you 😊👍

[@ranipushpalatha4178]

Thankyou

[@ZeeshanAli-bv2rs]

Amazing 😱

[@DKBiosolutions]

Thank you 😊👍

[@lalruatpuiithawmte2188]

in microscope chu tha hle mai 😶😬

[@raicheltheressajohn2060]

well explained

[@DKBiosolutions]

Thank you 😊

[@batistacassiusnongphlang7106]

Thanks

[@DKBiosolutions]

Welcome,😊

[@papiyadas3103]

It was very difficult for me to remove the salivary gland from Chironomous larvae 😓😓😓Sir suggest me some tricks 😓

[@DKBiosolutions]

Hello Papiya Das...
Believe me, it is very easy to remove salivary glands from Chironomous larvae than Drosophila larvae.
Follow these tricks:
1) Observe larvae under dissecting microscope and locate the Head.
2) Use two sharp pointed needle to hold head segment and remaining body.
3) Pull head segment forward with first needle gently. Don't pull second needle with whom you have grabbed the body.
4) You will see along with head segment, gut is coming out. Don't break it by pulling hard and long.
You will see two rings (Salivary glands) attached to the Gut.
Try till you get it. Best luck 😊👍...

[@papiyadas3103]

@@DKBiosolutions thank you so much sir for the reply...I will try my best to do the practical 😊 next time

[@kanizfatma4473]

Thank you so much for this technique
Sir pls.. upload the video of the preparation of lampbrush chromosome

[@DKBiosolutions]

My pleasure 😊... We have midterm holidays here... I will try to prepare that video as early as possible...

[@gmgokul9913]

Thank you sir super

[@DKBiosolutions]

😊👍

[@rohitpankar7412]

Nice 😃

[@yogeshu339]

Please meiosis ki slide banana bhi bataye

[@DKBiosolutions]

Colleges abhi thik se start nahi huye hai... Regular start hua ki bana dunga... 👍😊

[@yogeshu339]

@@DKBiosolutions thanx

[@yogeshu339]

@@DKBiosolutions please cheek cell ki vital stain slide banana or mitochondria ki slide banana bhi bataye

[@DKBiosolutions]

My pleasure 😊👍

[@DKBiosolutions]

Jarur... Thank you for suggestions 😊👍

[@humblee2]

Sir pls do videos on grasshopper meiosis

[@DKBiosolutions]

I will try my best 👍

[@arpita707]

Btt sir..ye toh msc ke practicals hain.

[@DKBiosolutions]

Bsc zoology me hai na...

[@atulsonkamble4854]

Thanku sir

[@yogeshu339]

Aceto alcohol banana bataye

[@DKBiosolutions]

1:1 aceto-alcohol means mix equal parts of each liquid... Example- 1ml of acetic acid mix with 1 ml of alcohol...

[@yogeshu339]

@@DKBiosolutions thanx so much

[@rabikhan5023]

Sir plz 🙏 tell the observation in text form 🙏

[@DKBiosolutions]

Because polytene chromosomes are extended and consist of so much DNA, they are easily visible under the light microscope. A useful feature of these chromosomes is that they have a pattern of dark and light bands, like a bar code, which is unique for each chromosome.

[@rabikhan5023]

@@DKBiosolutions Thanks a lot 🙏 😊 ☺

[@DKBiosolutions]

My pleasure 😊👍

[@joselinares4218]

8:25 the best spreading of arm chromosome

[@DKBiosolutions]

😊👍... Please suggest for improving chromosome arm spreading...

[@atulsonkamble4854]

Ata he practical mi dole zakun pn karu shakto....

[@rohitpankar7412]

Dole ughade teva 😜

[@lalruatpuiithawmte2188]

offline a kan tilo hi ka lawm 😂😂

[@sujaygowda4358]

🔥🔥🔥

[@kingprince0727]

Thnxx😮

[@Wordboost087]

Humne salivary gland hi q liya

[@DKBiosolutions]

Polytene chromosomes baki cells me nahi milenge...

[@lady9265]

الله يسامحك يا دكتورة فهمت nothing

[@DKBiosolutions]

إنه في منهجنا. شعرت بالسوء أيضًا ، لكن ليس لدي أي خيار آخر.😔

[@lalruatpuiithawmte2188]

aw tho te u 😂