Co Immunoprecipitation | Immmunorecipitation | Co IP Assay Principle | Procedure | Technique |

This video lecture describes
1. What is Immunoprecipitation (IP) ? How immunoprecipitation is used for the detection of protein of interest?
2. What is Co immunoprecipitation (Co-immunoprecipitation) (CO-IP)?
3. What is the principle and procedure of immunoprecipitation and co-immunoprecipitation ?
4. How Co immunoprecipitation (Co IP assay) can be used to study protein protein interaction ?
5. What is the difference between immunoprecipitation and co immunoprecipitation ?
6. Co immunoprecipitation steps
7. immunoprecipitation steps
#biologylectures #molecular_biology #biochemistry
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[@quangvinhnguyen114]

As a freshman who just start to read reseach articles, I have to look up and self-study a lot of concepts and methods of technologies that I was rarely taught at lower education level
So I really appreciate videos like this
Thank you!

[@BiologyLectures]

You are most welcome. We are so happy that out videos are useful to the people like you. We wish you all the best with your studies 🤗

[@ishmaellaryeatetteh9357]

When we use magnetic beads, we don't need to spin the mixture. We use a magnet to pull them to the side of the tube. We spin when we use agarose beads.

[@BiologyLectures]

Thanks for your insights 😊

[@educationinfo5080]

Well done 👍

[@BiologyLectures]

Thank you 😊

[@Zhi-u6j]

Great explanation thanks

[@BiologyLectures]

You're welcome!

[@dikshyapanthi7681]

Excellent explanation sir ❤

[@BiologyLectures]

Keep watching

[@investincrypto2859]

Excellent 👌

[@BiologyLectures]

Thank you! Cheers!

[@markrobinson9676]

Excellent explanation 🙂

[@BiologyLectures]

Thank you so much.

[@pmmp2801]

So what is the difference between both techniques? It seems as the same procedure, how do you make sure youre keeping the protein-complexes toghether?

[@conleydickerson5029]

Immunoprecipitation is used to isolate just one protein. We have an antibody for protein X. Protein X will attach to our antibodies which are attached to a bead. We can then pull these down with centrifugation and isolate protein X. Co-immunoprecipitation is used to determine protein protein interaction.s We would have a bead with an antibody for protein A. Once protein A is on our bead, we would then expose it to a solution of proteins. If our hypothesis is that protein A binds protein B, we will put protein B in the solution with the beads which have protein A attached. Then we will pull down these beads and run a something like a western blot to determine if protein B is present. If it is present, we know that it would only be present because of its attachment to protein A.

[@dianaasante8247]

thank you

[@BiologyLectures]

You are most welcome 🤗

[@monikamoitra4531]

What is the difference between immunoprecipitation & ELISA?