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Hello, thanks for the explanation But I have a question, how will the detector differentiate between the reference and the sample beam, if they both merge and enter the detector as one single beam?
There is a beam chopper (not pictured) - a rotating disc with sections: an opening, a mirror and a black one. When the disc rotates, it intermittently permits the beam onto the sample, reflects the beam onto the blank or completely blocks the beam from the detector. But I never understood how exactly all that worked.
Hi +arib shaikh. Thanks for watching the video. Hope you have enjoyed watching it. There won't be a problem to use multiple detectors as you suggested. But the reason to use a single detector is to reduce errors arising due to mismatch of the characteristics of detectors. Every detector reacts slightly different to the same input light that falls on it. This can induce errors during final calculations. So it is a better option to use a single detector as it's characteristics will be matched for both the sample and reference signal. One more benefit of using a single detector is that it saves the cost of the equipment and added circuitry. We hope your doubt has been clarified. If still any queries, feel free to comment. Stay calm and Keep learning.
There are losses of incident beam mainly due to absorption of the sample, but also due to the solvent (matrix), but also due to refractions on each interphase: air/cuvette wall/solvent/wall/air. If you keep the blank cuvette empty you have air/wall/air/wall/air infractions. So, no, the blank cuvette should have solvent (and the matrix of the sample).
In double beam spectrophotometer, the same light radiation needs to pass through the sample and the reference simultaneously. This is possible only if both the analyte solutions are kept in the sample holders at the same instance. So, two different positions are used, one for sample and the other one for reference solution. If same position is used for both the sample and reference, it is called single beam spectrophotometer. Hope this clarifies the doubt :)
We will try to make a video on the said instrument. However if you are looking for a video on fluorimeter, you can check the following link th-cam.com/video/B22ZsUOFM98/w-d-xo.html. This link explains spectrofluorimeter. The working of double beam photofluorometer will be almost similar to it however with the change that the sample and the standard will be placed simultaneously and measurements are taken.
Get amazing content and support on Academic Courses, GATE Courses, Professional Courses and Career with Ekeeda. Go to ekeeda.com/ platform and register with the referral code EAPHAR74. Use the coupon code Ninja10 on purchase for special discount!
We have an exciting news for everyone! We have developed a course on PLC basics in collaboration with Internshala where you can get good discounts using the coupon code ISRP16947850. Go check it out here- trainings.internshala.com/plc-programming-training
Dope! The explanation are easy to understand,I really appreciate it🙏🏾🙏🏾
Best explanation of a double beam UV-VIS spectrophotometer...Thank you so much :)
Thanks Rachna Jadhav. Glad you liked the video :)
Hello, thanks for the explanation
But I have a question, how will the detector differentiate between the reference and the sample beam, if they both merge and enter the detector as one single beam?
There is a beam chopper (not pictured) - a rotating disc with sections: an opening, a mirror and a black one. When the disc rotates, it intermittently permits the beam onto the sample, reflects the beam onto the blank or completely blocks the beam from the detector. But I never understood how exactly all that worked.
My doubt is why's it necessary to use two samples simultaneously?? What if a single sample solution is involved??
Because it deals with variation in light intensity better
In reference chamber which item should be put either sample or stard or blank any idea??
the reference , is the blank right?
Can we use two detectors ,one for sample n another for reference or single detector is enough?
Hi +arib shaikh. Thanks for watching the video. Hope you have enjoyed watching it. There won't be a problem to use multiple detectors as you suggested. But the reason to use a single detector is to reduce errors arising due to mismatch of the characteristics of detectors. Every detector reacts slightly different to the same input light that falls on it. This can induce errors during final calculations. So it is a better option to use a single detector as it's characteristics will be matched for both the sample and reference signal. One more benefit of using a single detector is that it saves the cost of the equipment and added circuitry. We hope your doubt has been clarified. If still any queries, feel free to comment. Stay calm and Keep learning.
Reference sample here is the Blank sample, not the Standard sample, right?
Good! Keep it up!
Very helpful... And easy to understand. Thank you
Nice video,
Can we use the "reference" cell with only air inside the cuvette and do a blank with the "sample" cuvette containing our buffer.
There are losses of incident beam mainly due to absorption of the sample, but also due to the solvent (matrix), but also due to refractions on each interphase: air/cuvette wall/solvent/wall/air. If you keep the blank cuvette empty you have air/wall/air/wall/air infractions. So, no, the blank cuvette should have solvent (and the matrix of the sample).
why we use to position one for sample and one for reference if we use same position for sample and reference is that ok or not ?
In double beam spectrophotometer, the same light radiation needs to pass through the sample and the reference simultaneously. This is possible only if both the analyte solutions are kept in the sample holders at the same instance. So, two different positions are used, one for sample and the other one for reference solution. If same position is used for both the sample and reference, it is called single beam spectrophotometer. Hope this clarifies the doubt :)
Amazing ✨✨✨✨
amazing
i mean to easy to get in brain
Saviour🙌
Thanku sir!
Anyone can told me about Half beam spectrophotometer
okayy tysm
😃
plz explain double beam photoflurorimter
We will try to make a video on the said instrument. However if you are looking for a video on fluorimeter, you can check the following link th-cam.com/video/B22ZsUOFM98/w-d-xo.html. This link explains spectrofluorimeter. The working of double beam photofluorometer will be almost similar to it however with the change that the sample and the standard will be placed simultaneously and measurements are taken.
wow
sorry for so many msgs My net was not working properly
sorry for so many msgd My net was not working properly
wow
Glad you liked the video +Gavin Dsouza :)
hey