Immunohistochemistry in Normal Skin: Cytokeratins

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  • เผยแพร่เมื่อ 29 ธ.ค. 2024

ความคิดเห็น • 17

  • @JMGardnerMD
    @JMGardnerMD  3 ปีที่แล้ว +1

    A complete organized library of all my videos, digital slides, pics, & sample pathology reports is available here: kikoxp.com/posts/5084 (dermpath) & kikoxp.com/posts/5083 (bone/soft tissue sarcoma pathology).

    • @thegood9
      @thegood9 2 ปีที่แล้ว

      Dr. Gardner, you are a lifesaver and a GREAT teacher. As a clinician in this field, I have found your library late, but, all your content has been really helpful, useful, and quite edifying. Great images, sir. Thanks once again. I will support you in all ways possible!

  • @hesamb9135
    @hesamb9135 3 ปีที่แล้ว

    I learn a lot from your lectures. Truly appreciate your time and help🙏

  • @wallendegen1
    @wallendegen1 6 ปีที่แล้ว +4

    6:56 I recently attended a skin seminar where it was demonstrated that those globules you mentioned are amyloid deposits which arise from degenerate keratin in the setting of certain neoplasms. Congo red and EM findings were positive for amyloid in the globules.
    Just thought to share this Jerad Gardner.

  • @rmetal2792
    @rmetal2792 6 ปีที่แล้ว

    I am a histotechnologists. Thank you for teaching me Dr. Gerard. I am learning a lot and i like to learn new things esp immunohistochemistry. I wish you can do more videos about IHC. :D

  • @azgonz56
    @azgonz56 5 ปีที่แล้ว +1

    The diagnosis of Cutaneous Mastocytosis, the underlying Chronic disease thereof (Mast Cell Activation Syndrome [it's Disorder/Disease]), and the Neoplasia (Mastocytoma [very rare incidence for both children and adults]) should not entail a possible misdiagnosis when using immunohistochemistry as the staining mimics high molecular weight of keratinocytes, correct? Although mastocytosis is best diagnosed by a bone marrow biopsy, histopathology can easily indicate the disease's morphology with these characteristic keratin markers. The role of a Mast Cell is immunological and therein carries significant molecular structures such as Immunoglobulin E (IgE) but other mediators, especially Tryptase, could potentially have a similar molecular signature, weightwise, also. This cell is "loaded"....Cheerz AG

  • @wallendegen1
    @wallendegen1 6 ปีที่แล้ว

    Very helpful basics for trainees.

  • @cardiomeglee
    @cardiomeglee 11 หลายเดือนก่อน

    Do the eccrine ducts stain positively for LMCK, or is it limited to the eccrine coils (predominantly the inner apical layer)?

  • @shannonnoseworthy
    @shannonnoseworthy 2 ปีที่แล้ว

    Thanks for this Jerad! What are your thoughts if you see 70 scattered mast cells per millimeter square? That's with CD117 staining from the back.

    • @JMGardnerMD
      @JMGardnerMD  2 ปีที่แล้ว +1

      That sounds higher than usual for normal skin, but I see increased mast cells often in a variety of reactive and inflammatory processes that are not mastocytosis. For mastocytosis, I like to see mast cells clustering around dermal blood vessels and of course in the proper clinical context. But Mast cell disease can be subtle and difficult to diagnose sometimes.

    • @shannonnoseworthy
      @shannonnoseworthy 2 ปีที่แล้ว

      @@JMGardnerMD Thank you so much 😊

  • @loudefeo2510
    @loudefeo2510 6 ปีที่แล้ว

    Thank You

  • @alinazar4928
    @alinazar4928 6 ปีที่แล้ว

    Thank you very much sir

  • @azgonz56
    @azgonz56 5 ปีที่แล้ว

    Immunohistochemistry is dealrly helpful in a diagnosis but rather unspecific and time consuming.. Microarray is superior and abilizes a diagnosis and thereby Treatment,as every disease has a easily recognizable signature...Cheers Asrst Gardner..Es sei unsere Gesundheit
    Thanks
    Hi

  • @sethivrunda
    @sethivrunda 6 ปีที่แล้ว

    Nice...