HPLC - How to read Chromatogram Easy Explained - Simple Animation HD

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This video explains the concept of the chromatogram. Don't focus on the numbers as the numbers are fiction. It's all about the basic principle of the chromatogram.
Learn the basic concept of High Pressure Liquid Chromatography
01. Introduction
• HPLC - High Perfermanc...
02. The Mobile Phase
• HPLC - What is the fun...
03. The Stationary Phase
• HPLC - The Stationary ...
04. Normal Phase HPLC vs Reverse Phase HPLC
• HPLC - Normal Phase v...
05. HPLC Isocratic vs Gradient analysis
• HPLC - Isocratic vs Gr...
06. HPLC - UV-VIS detection of analytes
• HPLC - UV / VIS detect...
07. HPLC - How to read a chromatogram?
• HPLC - How to read Chr...
08. What is the difference between HPLC and GC?
• HPLC and GC - what is ...

[@HikariKamiya3]

OMG I really love all of these videos 'cause it's helping me a lot for tuesday's final exam

[@Lostpanda123]

For those struggling with calculating the concentration for compound X; notice the ratio difference between 200mg/l and 250mg/l, the pattern is that for 1 mg, the peak area increases with 1000. Therefore its only a matter of multiplying peak area for compound X with 1mg/1000... The answer is as stated in the video: 207,757 mg/l

[@user-cj8zj8sc2g]

So you divide by 1000 in order to get the concentration but the Peak area is = to 200,000? So how has the answer become 207,757?

[@whitealger.9540]

@@user-cj8zj8sc2g No,the peak area is 207757(p.area of compound X).
u have every 1mg increases with1000 p.area,so how many mg in 207757p.area?
So :207757x1/1000=207,757

[@dr.mohammadasifiqubal2455]

Your all videos are well explained. I really enjoyed. Keep it up and upload more

[@TutunChanda]

Lovely.. most easiest explation. Thanks a lot

[@55prasenjit]

Excellent explanation! Thanks!

[@ayeshasyed1038]

Loved the way of explanation....hats off...keep up the good work

[@superluvdub]

Wow! Thank you. I wish you were my science teacher

[@2357y1113]

Yo dat peak area was THE SHIT, bro!
11/10 would analyze again.

[@aposvlah1855]

2357y1113 what is mg/l ?

[@nadiaazman6980]

miligram per litre. a concentration unit.

[@BraKahan]

THAT IS PPM YOU PEASANT

[@1p53gen]

very well explanation. Thank you.

[@Reni8705]

Thank you for the fantastic vid 👍🏽😎😎

[@_clarisse_manishimwe]

Thank you for this!

[@renegade839]

Thanks for the video. 👌

[@minalbhalerao]

Thank you for this video. It is helpful.

[@norsuhadah6402]

thank you very muchh

[@atikaadnan2019]

tqsm for this well explained video. it helps me a lot

[@NitinKumar-ez3cc]

Very good job sir ji
Thank you so much

[@mariareginasoares1662]

amazing, thank you!

[@ahmedibrahim605]

Clearly explanation cheers

[@tugceceyhan9066]

It was so helpful, thanks a lot:)))

[@haroun8332]

I liked to watch more of your videos in other interesting technics

[@phuonggiang1545]

this saves my assignment >< thanks a lot

[@Kirann2487]

good...very well explained..

[@rr3775]

This was very helpful to me. I have a question for you. I need to create a tool (either in R, Excel or python) to calculate and plot the critical peak pair (pair with the lowest resolution) for a given set of parameters. Firstly, what is "resolution" in this context? Also, can you point me to literature that would help me come up with this tool?

[@vanillalatte1010ify]

thank you!!!

[@wanmuhdhatim2958]

LOVE U!!!

[@khalidhakimi4839]

Thank you this is what I want

[@13x97]

Love It .♥

[@vinzky89]

Thank you so much.

[@averymerta9304]

By far the best video I’ve ever seen for explaining this in simple terms, I now get it, Thank you ❤

[@shusilaghimire8354]

Superb!!

[@nagarajuk7494]

well , super ,tq so much

[@nadiaazman6980]

Hi Sir,
In your video, you stated that the peak area represents the amount of compound that has passed the detector. Does this statement apply to the chromatogram with mV as y-axis as well? Looking fwd to your reply. Thanks

[@lawalsuleiman1332]

Great job

[@makayacine9314]

simplicity is the ultimate sophistication

[@ShivaBioTechnical]

अति श्रेष्ठ व्याख्यान धन्यवाद

[@SulaimanAlnaimat]

really
amazing

[@examtime2693]

Explanation is good
But it’s so irritating to watch video without voice

[@aslamhayat8087]

this is good and informative video

[@hamzahamza-cf8qi]

Thank's :)

[@nadeemiqbal4742]

Nice elaboration.

[@RajeshKrRanjan]

I uploaded practical video on HPLC and HPTLC analysis using software OpenLab and Visioncats...

[@Mega-nz9pf]

Thank you for saving my grades at Uni

[@niftypubgmobile2261]

Good luck at uni

[@200heatblast]

super ji I am watching this series of video which is more informative than my book I am preparing for analytical engineering test using this video

[@ajaymedicine]

Very helpful

[@pharmaboy1510]

Excellent

[@emmanuelmichael462]

What else does the peak height indicate if it doesn't guarantee more concentration than any other below jit

[@jebusslaves9297]

What does it mean if the baseline is not straight? Is it an error with sample prep, or an error with detector?

[@helix7564]

+Jebus Slaves try to adjust pH, or other columns. It could be many reasons. The chemicals react with the filling materials, or two components are not separated thoroughly, or it could be isomers, or try to adjust the gradient time of mobile phase.

[@MrFeelxd]

In my case, I had also troubles in not staight baselines, and the reason was preassure, a strong hydrophobic compound in Reversed phase HPLC can be stuck at the begining of the column, one way to solve it is turning around the column. By doing this, they can be eluted easily.

[@lokmanhilmi2627]

easy to understand

[@peterlauridsen8403]

what does it mean that a column has been calibrated?

[@sonison668]

Where do you get the retention times from?

[@sonison668]

as in the reference list of compounds and retention times

[@isiakaowolabi9059]

I did my master's degree in analytical chemistry but synthetic organic chemistry for PhD. how to determine syn:anti from hplc spetra?

[@aposvlah1855]

chem chem so what is mg/l ? how is this concetration? is this ppm?? why the peek 277456 pf compound x is 277,456 mg/l ??

[@isiakaowolabi9059]

kindly help me,
what is the exact formula to calculate syn:anti from hplc spetra? this video explanation not clear to me. from retention time, area, peak and concentration values, how to determine syn:anti of chiral compound?

[@taotao2623]

I think chiral compounds also have different R-time, maybe you can find the explaination from instrumenal analysis

[@fheed-almzbed1744]

Thanks but it will be great with voice

[@intelligentemindforpeace952]

👍🏻

[@xxanimefanxx11]

So how do you calculate the concentration of the unknown sample?

[@alexsenior9385]

Divide the area of unknown by area of known then multiply by the concentration of known. Hey presto!

[@cristhianforero2731]

Y=mx+b

[@roadtophd-innovators5051]

Can you please provide raw hplc data file so that , can learn to interpret them

[@zygaf6252]

1:21 why all samples are named C1?

[@TheIHaZZarD]

A bit late but it's because it's the same sample (Caffeine) but with an increasing concentration with the purpose of making the calibration curve.

[@nassz23]

Which phenomenon couldn explain the bad baseline?

[@ivanmegafanboy1981]

Maybe the mobile phase absorbs some of the light from the detector, so it is set in a bad wavelenght; or the sample contains a wide mixture of compounds.

[@zeeshanqasim8925]

does anyone have audio complications?

[@younesbakache]

Geaaaaaaaaaaaaaaaaaat

[@user-nc5yg3ki3n]

OK

[@Karamojobell94]

Duke city

[@Sun-soilproductions]

I wish there was talking

[@realdamageboy]

not even close to HPLC

[@isiakaowolabi9059]

I did my master's degree in analytical chemistry but synthetic organic chemistry for PhD. how to determine syn:anti from hplc spetra?

[@munirothmanali9461]

Hi sir,
I catch you very nice but my question is to you is you said that higher peaks doesn't mean higher concentration .how?
And if you recall in normal way as compound conc increase its give higher peaks.