Reverse Transcriptase: RNaseH, DNA Polymerase, RNA Virus genome replication

[@vijjumahankali4297]

Thank you sir😊

[@Dr.DNA-Primer]

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[@anahitaaghazadeh]

perfect

[@Dr.DNA-Primer]

Thanks do subscribe for more updates

[@Dr.DNA-Primer]

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[@KrishnaKrish-m8s]

Sir I have seen your two videos on reverse transcriptase.. But I have a doubt reverse transcriptase enzyme need template OR not for synthesis of cDNA. If need template than which template

[@Dr.DNA-Primer]

Hi, During the retroviral infection, by using Reverse transcriptase RNA is converted into double standard DNA and that is integrated into host genome. Eukaryotic mRNA will be used as template to synthesize cDNA by employing Reverse transcriptase.

[@KrishnaKrish-m8s]

@@Dr.DNA-Primersir reverse transcriptase requires a primer,
which in the case of retroviruses, is a tRNA molecule bound at a specific site
(the primer-binding site) close to the 5' terminus of the viral RNA

[@Dr.DNA-Primer]

Yes