Bacterial Transformation
ฝัง
- เผยแพร่เมื่อ 19 มิ.ย. 2024
- Cold Spring Harbor Laboratory’s DNA Learning Center presents this course as a service to help engage teachers and students in China during the coronavirus school closures.
The bacterial transformation experiment illustrates the direct link between an organism's genetic complement (genotype) and its observable characteristics (phenotype). A gene for antibiotic resistance is introduced into the bacterium E. coli. Following overnight incubation, transformed bacteria are compared to unexposed bacteria for their ability to grow in the presence of ampicillin.
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I am 35 years old, and I am learning a lot from this, thank you.
Thank you, thank you, thank you for doing this video. I was so incredibly disappointed that my students are not able to do this wet lab. They love seeing their bacteria glow. I appreciate the considerate attitude you all have to have to film this and make it available to educators like us. Tarrant County College Northeast campus microbiology says thank you.
We appreciate you taking the time to reach out. We miss the classes and look forward to seeing everyone soon! Wishing you all well!
Surely students handling say e-coli would give the disinfectants industry such a boost. And so the pharmaceutical companies for anti -biotics !
Imagine what fun it would be if she "just snipped out " an Antibiotic resistance gene " and shoved that in there !. - or a "covid 19" spike protein " gene. Then call it a "vaccine" and tell all the "mice" that it will "protect them" if injected into the shoulder muscles !. No even better MANDATE that they must do that or everything they own is taken away for public health safety. Yes that's sure to be very popular - with pharma shareholders, CEO's etc. who will then all be worshiped and given enormous pay rises for "saving the world from the terrible Thing from "Wuhan", for instance!
And all the green ladies with horrible falceto voices can tell any survivors how it was done - assuming that they all "vaxxinated" themselves with the Thing resistant gene.
I actually got to do this lab earlier this semester and it was amazing to see glowing bacteria! Had to watch to refresh my memory for my lab final this week and it worked. Thank you. Great video!
My name is Chidera, and I currently work at DNALC, Nigeria as a Research assistant. I can't wait to come over to DNALC NY to meet you and all the amazing people that work there. Meanwhile, thanks a lot, Erin for this amazing video. I did learn a lot.
And, I particularly love the passion with which you teach. So cool!
Amazing Profesor! I was very dissapointed that I could not do this experiment because of the COVID-19. Although I was very exited and the class was amazing!! Hope to see more videos!!
So happy you enjoyed the lab! We appreciate all the support and feedback. Thank you!
@@dnalearningcenterchatmoder1379 Great.
Thank you! As a student that can´t go to a lab right now, I really appreciate this videos.
If you could add a spanish cc, it would really help to get this video to more students or add a contribute translations option so it can be more accurate
This how one should teach perfect combination of practical and theory, mam can you can vedio on RNA interference with its lab experiment also on sequencing of band in the gel electrophoresis
Nice! Made this so easy to follow along. For some reason my Prof thinks not teaching is the best method of learning so I am very grateful for this!
Hi, I'm a Lab Tech in a high school in Australia, and I was wanting to know where you purchased your collapsible DNA modal from? Thanks
Excellent presentation. This is really helpful for all kinds of viewers. Thank you so much. Expecting more videos like tis.
Thank you so much.....this was so perfectly made...
This is so helpful. Thank you. It is a great video.
Thank you very much. This was very informative..
This is very clear. Thank you.
This was best lecture ever 👌 thanks ♥️ alottt teachers !
I had to watch the explanation over and over again in order for it to make sense. Am I completely incapable or is this normal? The teacher is phenomenal btw.
You are definitely normal! Let us know if you would like any clarification, we are happy to help out. Thank you for the comment!
Thank you! if you are teaching it would be great to transmit the importance of wearing gloves in the Lab. Especially when working with 'Live' organisms. :)
I hear an Oscarbuzz for this lab 10/10. I may fall in love a little bit with her 🥺❤️
Thank you for the support!
we performed this experiment and it was very disapointing since we didnt get the result of a glowing bacteria E.coli. we left it overnight and during the morning when we place our petri dish under UV light there where no signs of bacteria culture in LB/AMP and AMP/ARA. what could be the cause of this, since you said that the plasmid that has a GFP genes does not affect the ampicilin.But in pur petridish there were no bacteria?.
Incredible! 💯🥺 Such a great lab!
Great Teacher.
Hey, I'm curious would the UV light harm Ms McKechnie, the cameraman and anyone else in the room?
Thanks.
Thank you for your question! As it turns out, there are three main types of UV light: UV-A, UV-B, and UV-C. UV-A has the longest wavelength, and therefore, has the least amount of energy. UV-A is the type of UV light produced by what we commonly refer to as "blacklight"--and thus is safe for Ms. McKechnie to handle as she does in the video. UV-B and UV-C type UV lights, which have shorter wavelengths, have much more energy, and are viewed as mutagens (they have so much energy that they can damage or mutate your DNA in harmful ways). When we use those higher energy UV lights, we have UV-blocking covers to protect us and others in the lab from the harmful effects of UV exposure. We sometimes also use face shields, or protective eyewear, depending on the light source and how it is being used.
great video!
Sir please put all possible experiments related with biotechnology and genetics
Doing our best and thank you! Love the enthusiasm!
Thank you. Learning together.
Thank you
when is the next live tutorial ?
lol "we've heard about this coronavirus that is impacting you..."
where can we purchase materials
second to none, from university of Nottingham.
DNALC Live: Online educational programming to engage educators, students, and the public. dnalc.cshl.edu/resources/dnalc-live/
Am thinking if bacteria can make milk
Anyone
"Concerned for china" that aged poorly
Jesus loves you ❤
Great video, but her voice sounds like Minnie mouse is teaching the lesson. After awhile I have to give my ears a break, too much Disney!
Not for me, I love her enthusiasm!