Enzyme linked antibody can not be called secondary antibody. Rather it's a second antibody (it is also a primary Ab) that can recognise an epitope on the antigen that is different from the epitope recognised by the primary ( 1st Ab).
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Why the number of cells is equal to the number of spots? If many cells produce lots of antigen, it will form complexes very near one of another, therefore forming one large spot, so one spot isn't equal to one cell!
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GREAT EXPLANATION ! Thank you.
Simple and concise explanation, thanks so much!
You are very clear and specific! thank you so much!
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Very nice info sir
Nice explaination sir ! 👌
Enzyme linked antibody can not be called secondary antibody. Rather it's a second antibody (it is also a primary Ab) that can recognise an epitope on the antigen that is different from the epitope recognised by the primary ( 1st Ab).
Greetings from France
Please make a video on ELAST assay, and the difference between ELAST and ELISA. This was a great video, thank you
Sure
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Great explanation 😍. Thank you so much
Please make a vedio about the complement fixation test 😊
Tq sir to clear my all doubt about in this topic 🤗
Brother i recommend u to teach or start life science classes becoz ur ocean of knowledge
Many students will get benefit
Ur way of explaination is totally different and clear
Thanks for this explanation 😀
Very nice
Thank you
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Thank you ❤️❤️❤️❤️
Legend
simplest illustration so far!!! great!
Why the number of cells is equal to the number of spots? If many cells produce lots of antigen, it will form complexes very near one of another, therefore forming one large spot, so one spot isn't equal to one cell!
To eliminate this problem.. sample is serially diluted...
Thank you
thank you!
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Where is ELISA lecture...
U can find it on my channel.. or you can search- ELISA sajid shaikh on youtube