I would advise against having a fan, you want to have no air movement, preferably a closet or big box with plastic curtains so no outside air gets in after you spray everything down it stays sterile
Please upload the vdo of next step and please tell me what is the liquid fertilizer and after mixing thae agar with fertilizer will u keep it in refrigerator for make it solid
Home tissue culture medium (Bridgen and Brand, 1985). Medium Constituent Amount1 Table sugar 1/8 cup Tap water 1 cup All-purpose soluble fertilizer 2 1 cup of stock solution Inositol tablet (250 mg)3 ½ tablet Vitamin tablet with thiamine ¼ tablet Agar flakes 2 tablespoons 1 This mixture makes 1 pint of medium. 2 Prepare the fertilizer stock solution by mixing ¼ tablespoon of a balanced, water-soluble fertilizer, such as 10-10-10, in a gallon of water. 3 lnositol is sometimes sold as myo-inositol.
Bukan la xbagus guna bahasa inggeris. Tp dalam menjelaskan pengetahuan pasal sains dan teknologi kita kurang video dlm bahasa melayu. Kalau dlm bahasa Inggeris bersepah. Xkn nk harapkan mat Saleh wat video ckp melayu plk. Fikir2 kan lah.. apa pun video yg baik.. 👍
Terima kasih bang untuk peringatan, video ni saya buat untuk project assignment so memang kena guna bahasa inggeris, maaf kalau kurang jelas, hv a nice day✌️
@@HaiqalYaacob There is NOTHING wrong with your English. If you didn't speak in English, I would not watch this video at all. Please quantify the amounts so that I can carry out the experiment. All very clear and simple. Exactly what I want.
Very nice content ! But it would have been even better if there were not any music in the background. Thanks
Softer or less end of world
Hi! Very interesting video! Is there a follow up video of the plantlets? Was there any contamination? Best regards and keep up the good work! ✨🌿
Very nice
so far the best tissue culture tutorial I've been able to find!! what's the concentration of Clorox bleach in the plant wash?
Thank you! I use 20% of clorox for the plant wash
@@HaiqalYaacob Kuat or Clorox? Because 20% Clorox would very quickly kill a plant.
@@HaiqalYaacob maksudnya, clorox beli kat kedai, kita dilute 20% v/v ker?
Bottle 100%, dilute to 20%
I would advise against having a fan, you want to have no air movement, preferably a closet or big box with plastic curtains so no outside air gets in after you spray everything down it stays sterile
Nice infrmation.
sir, your practical is good .please give quantity of meterial, and name of fertiliser.
Salam bang. Saya lagi fans dari Jawa tengah. Terima kasih untuk info. Semoga abang sukses
Waalaikumussalam, thanks for the support, salam kenal
Please upload the vdo of next step and please tell me what is the liquid fertilizer and after mixing thae agar with fertilizer will u keep it in refrigerator for make it solid
so can you show the result?
Thanks for posting.
If you mention elements like agar chlorofox purchasing link will be great
Do you have a link to purchase the auger?
What is the ratio of agar : fertilizer, if I may?
Also, are there people here that are actually doing tissue culture at home?
I’m about to do my first batch of tissue culture wasabi plants :D
@@ArtyMars That is great to hear. Can you keep us posted? Or, will you be making any blog post or video about it? Would love to follow.
What fertilizer u use...in what typical name or specific condition of fertilizer, since there are so many different fertilizer to chooses
It would be nice if i have the results...
Hi Can you give a list of the items needed please? Saran wrap is cling film???
Very very good information
Can you help me bro
What's your water and fertilizer ratio pls?
Where is the best place to incubate the plant?
Salam bro, terima kasih dengan perkongsian ilmunya. Mantap! 👍👍 This is The Project I'm gonna be working on! Awesome walkthrough, bro! 😉
Waalaikumussalam, thank you so much!
quantity of agar ane fertilizer ???
Pliz tell me name of the fertilizer and Agar and amount of water,agar and fertilizer should we use one time for the necessary
I use NPK green fertilizer
How many grams of NPK Fertilizer? Thank you.
Thank you
what is agar? Is it the thing used in place of gelatin or china grass? and can I use any bleach
Hallo, can you use any kind of filtilezer, because I will try it .and een you make the agar ,you can use it or wait for it is hard
I recommend you to use npk green fertilizer, and for the agar we need to wait until it is hard
how do you make sure the paper towel is sterilised?
Good question, since the experiment was conducted at home, I used a pressure cooker instead of an autoclave
thank you too
can we use corriander or any other?
How do you avoid contamination in open air? Should be done in a still air box or laminar flow hood.
Thank you! Very informative video.
Show us the results please
Bang kalau disinfectant tu pakai hydrogen peroxide ble ke?
How to add hormones naturaly
Where is the result?
How much Clorox?
Does it matter which side of the stick goes in? Like is there an top or bottom
Wow
Hi can you give the amount of solutionised NPK fertilser please?
Salam bro.. Result camner? Jadi x?
Wasalam, contaminated maybe due to improper sterilisation
Results ????
Contaminated, maybe due to improper sterilisation
@@HaiqalYaacob you should not use Fan inside chamber..
@@amtd3808 this is just a part of my assignment so I only use items that are already at home instead of laminar flow etc in a real lab
@@HaiqalYaacob ok...👍👍..Upload more informative videos with results ???
utk plant tissue culture bole ambik dari mna2 part plant ? contoh burle marx variegated guna daun sahaja
Boleh ambik mana mana part depends on mana yang highly differentiated
@@HaiqalYaacob meaning cutting leaf stem pun ada highly chance successful? sbb mostly aroid plant propagate guna node cutting ..
@@faizfitri3553 yupp most aroid plant guna node cutting cuma this time nak try part lain untuk test mana yang lagi cepat tumbuh
@@HaiqalYaacob ooo so mmg bole guna daun sahaja ataupun leaf stem utk kaedah tissue culture?
@@faizfitri3553 boleh but depend on what plant
yg bahasa melayu xde ke
what is chlorox??
Yes quantity.
Very risk contamination
Plant tu tak takut ke tgk abang.
Nasib baik awak takde dalam video, kalau tak orang yang terkejut
Jadi tak pokok tu bercambah?
Media contaminated sebab tak buat proper sterilisation, so tak jadi
@@HaiqalYaacob kalau utk caladium, guna daun boleh tak?
@@ArtfishStudio never tried caladium, ni just follow assignment instructions
👍👍
Thank you
Home tissue culture medium
(Bridgen and Brand, 1985).
Medium Constituent Amount1
Table sugar 1/8 cup
Tap water 1 cup
All-purpose soluble fertilizer 2 1 cup of stock solution
Inositol tablet (250 mg)3 ½ tablet
Vitamin tablet with thiamine ¼ tablet
Agar flakes 2 tablespoons
1 This mixture makes 1 pint of medium.
2 Prepare the fertilizer stock solution by mixing ¼ tablespoon of a
balanced, water-soluble fertilizer, such as 10-10-10, in a gallon of
water.
3 lnositol is sometimes sold as myo-inositol.
Little knowledge is always dangerous for others too.
Palm propagation method🌴🌴🙏🙏🙏🙏
i cannot understand any single word that speaks
Bukan la xbagus guna bahasa inggeris. Tp dalam menjelaskan pengetahuan pasal sains dan teknologi kita kurang video dlm bahasa melayu. Kalau dlm bahasa Inggeris bersepah. Xkn nk harapkan mat Saleh wat video ckp melayu plk. Fikir2 kan lah.. apa pun video yg baik.. 👍
Terima kasih bang untuk peringatan, video ni saya buat untuk project assignment so memang kena guna bahasa inggeris, maaf kalau kurang jelas, hv a nice day✌️
PLEASE REMOVE THE MUSIC
kadang bahasa penjajah ni ad bagus jgk tapi gunakanlah bahasa melayu kita supaya dapat membantu orang lain faham
Betul tu saya setuju. Tak semua orang faham bahasa inggeris ni
Maafkan saya, lepas ni saya janji akan memartabatkan bahasa ibunda tercinta kita!
@@HaiqalYaacob There is NOTHING wrong with your English. If you didn't speak in English, I would not watch this video at all. Please quantify the amounts so that I can carry out the experiment. All very clear and simple. Exactly what I want.
Kadang bahasa penjajah ni ad bagus jgk tapi gunakanlah bahasa melayu kita supaya dapat membantu orang lain faham
Tiberrr
Little knowledge is always dangerous for others too.