How to compute EC50 C50 in Dose Response fitting

This video shows how to perform dose-response curve-fitting on the single dataset and on replicate datasets, and compute EC50/IC50
#Originpro

[@user-or5bk4og1t]

Is EC50=IC50 value?

[@mynehc]

yes

[@user-or5bk4og1t]

Why change a1 and a2 to 0 and 100?

[@mynehc]

This is a preassumption the response percentage converges to 0 at a low dose and 100 at a high dose.

[@user-or5bk4og1t]

@@mynehc Can I not change it?

[@user-or5bk4og1t]

所以假如我的數據先算出是10%到80%那ic50是45%的對應值還是50%的對應值?

[@mynehc]

@@user-or5bk4og1t 45%. 计算最高点一半的x值。

[@user-or5bk4og1t]

@@mynehc 了解但是我怎麼看很多都是從50的地方拉線

[@soficlau7159]

th-cam.com/video/fZtFrxb0Z1s/w-d-xo.html because it checks all three boxes