High throughput sequencing

This DNA sequencing lecture explains High throughput sequencing method of DNA sequencing including illumina sequencing and ion torrent DNA sequencing. www.shomusbiolo...
DNA sequencing is the process of making a choice on the precise order of nucleotides inside a DNA molecule. It involves any system or science that's used to investigate the order of the four bases-adenine, guanine, cytosine, and thymine-in a strand of DNA. The arrival of speedy DNA sequencing ways has extensively accelerated biological and medical research and discovery.
Talents of DNA sequences has end up quintessential for basic biological research, and in numerous utilized fields akin to clinical diagnosis, biotechnology, forensic biology, virology and organic systematics. The fast speed of sequencing attained with ultra-modern DNA sequencing science has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous varieties and species of life, together with the human genome and different whole DNA sequences of many animal, plant, and microbial species.
An example of the results of automatic chain-termination DNA sequencing.
The first DNA sequences had been bought within the early Nineteen Seventies by tutorial researchers utilizing laborious ways headquartered on two-dimensional chromatography. Following the development of fluorescence-headquartered sequencing approaches with automated evaluation, DNA sequencing has turn out to be simpler and orders of magnitude turbo.
Up to date advances in DNA sequencing have revolutionized the area of genomics, making it possible for even single study companies to generate massive amounts of sequence data very quickly and at a appreciably decrease fee. These excessive-throughput sequencing applied sciences make deep transcriptome sequencing and transcript quantification, whole genome sequencing and resequencing available to many more researchers and projects. Nevertheless, whilst the cost and time were broadly decreased, the error profiles and barriers of the new systems vary significantly from these of previous sequencing technologies. The decision of an appropriate sequencing platform for unique types of experiments is an primary consideration, and requires a specified understanding of the technologies to be had; together with sources of error, error rate, as good as the speed and price of sequencing. We overview the crucial concepts and compare the issues raised by means of the current high-throughput DNA sequencing applied sciences. We analyze how future tendencies may overcome these limitations and what challenges stay.
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Thank you for watching

[@jesseleavitt2818]

Thank you for your dedication to open source information! It's amazing how many videos you have done. Would you be able to do a video on metagenomics?

[@shomusbiologyofficial]

+Jesse Leavitt thank you. Glad you liked my lectures

[@ithirstyforknowledge]

Suman, Your previous style of teaching was better in which you write and draw and we could understand better. this type is already there and net i full of it. we dont want this type of tutorial. Please go back to your style so we understand better. You write better. this picture is not even visible. Thank you

[@trasvisharma3628]

Agree

[@manthaneinpolu1450]

So what happens after the DNA thats bound to the beads via the adaptors are sequenced and have their primers trimmed? You mentioned that that after the primers are trimmed, something is broken down into smaller fragments. You referring to the DNA thats been trimmed of its primers? I’m stuck at around 9:24 on this video.

[@mayukhroy5393]

Sir,this video was helpful .

[@rajatranjan974]

How can I make a career in next-generation sequencing data analysis?

[@cuckookids999]

mostly best lectur broo......

[@HewanDemissie]

Thank you so much.

[@shomusbiologyofficial]

You're welcome

[@vijay.nmohakar5533]

why the sanger and maxam and gilbert dna sequencing are genereally not used in these method ?

[@maryamyousaf700]

Because they are more time consuming and costly

[@gunfighter3890]

Subscribed! Amazing guy

[@ap9686]

Thank you❤️

[@shomusbiologyofficial]

You're welcome

[@twinklesinghal6787]

Plz upload this video in detail and in hindi

[@nimraali9737]

super, thanks for sharing this

[@shomusbiologyofficial]

You're welcome

[@kiranburdak1834]

Super

[@dominikad.8571]

Raj is that you?

[@shomusbiologyofficial]

No. Not all Indians sounds alike.

[@souritradas6668]

Ok