Microplastic Processing Protocol
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- เผยแพร่เมื่อ 22 เม.ย. 2018
- A protocol video on how to process microplastic samples. Includes digestion methods, density separation methods, filtering methods, and how to identify plastic particles under a dissecting microscope. Can be adapted to fit into high school or college-level classes or for citizen science!
Water and sediment samples can be taken from stream, lake, and ocean ecosystems. You can also test tap water, bottled water, or personal care products. The protocol is highly adaptable!
Methods shown are adjusted from McNeish, Hoellein, and Kim.
Feel free to contact me if you're interested seeing in the written version of these protocols!
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Sieving 0:47
Digestion 1:50
Separation 3:35
Filtration 7:17
Counting 11:05 - วิทยาศาสตร์และเทคโนโลยี
So much educative and easy to understand.
I would be extremely grateful to receive the written version of these protocols.
If you send me an email, I can send you the protocol from this video. You can find my email under the "about" tab on my TH-cam page. In the meantime, the method in this video is similar to the method here marinedebris.noaa.gov/reports-and-technical-memos. The citation is Masura, J., et al. 2015. Laboratory methods for the analysis of microplastics in the marine environment: recommendations for quantifying synthetic particles in waters and sediments. NOAA Technical Memorandum NOS-OR&R-48
@@elizabethberg472 do you have a specific video/written protocol regarding counting of MPs present over filter paper under a microscope?
This video is great. I'm testing for MP's at my local WWTP and the visual demonstrations will make it much easier.
I'm glad it is useful!
Thanks Elizabeth !
I have question, is it okay if i collect sample using net than use 6%H202 for digestion and then pass it to whitman filter paper than oven dry and finally use sedgwick rafter cell.
I am using this procedure to count microplastic in fresh water
Thank you so much
What kind of filters did you use? And why? Thank you for answer!
Great vidoe.
Hi Elizabeth.
I am interested in reading an elaborate protocol for this experiment with details on quantitative information so it can be reproduced.
Where may I be able to find it?
hi, only supernatant use after density separation but you beaker contain more water?please tell ,i am going with soil samples and if i do;t filter it it contain soil particles ?is they are not problematic in next analysis or determination of mps by raman/ftir spectroscopy ...
Hi....Can we go for density filtration first and then go for wet oxidation....I m actually working on laundries....I want to avoid foam formation with peroxide..
Good Morning Maam Elizabeth Berg, May I have a written version of these protocol? Thank you very much.
Can you share the full protocol and what is the mass of soil taken? Please say
Thank you for this video, i want to ask about a counting metode, when we Can directly find a all mikroplastik with mikroskop in fiber glass whatmen? Or can we find the mikroplastik with visual before ?
I have not heard of counting the microplastic in water without filtering it first. I believe it would be hard to get an accurate count that way. Perhaps you could use that for estimating microplastics, but I think it would be difficult. I recommend filtering.
what should be the grade and pore size of glass fiber filter paper ?
Hi, I am a third-year student in the UK, and I'm currently starting my dissertation on Microplastics in the freshwater rivers. What sort of netting would you recommend?
We have used plankton nets. You need a net with a very small pore size.
can this method be used on cream type products? for example sunscreens and such?
Laboratory Protocol: Microplastic Sampling and Analysis
1. Sample Preparation:
In the field, collect samples using a drift net or plankton net, allowing water to flow through. Calculate the volume of water passed through.
Pour the net sample through stacked sieves, with the smallest sieve smaller than the net mesh.
Rinse the collected material with distilled water and transfer to a 300 mL beaker.
Dehydrate the sample at 75°C for 2-3 days.
2. Digestion:
Equip with a hot plate, stir bar, 400 mL beaker, 1000 mL graduated cylinder, heat-resistant gloves, 30% hydrogen peroxide, and 0.05 M iron solution.
Add equal portions of iron solution and hydrogen peroxide to the sample.
Heat the mixture at 75°C until boiling, then simmer for 10-15 minutes.
Repeat the process until no organic matter remains.
3. Density Separation:
Prepare metal stands, glass funnels, tubing, pinch clamps, aluminum tins, salt, and a balance.
Dissolve 6 grams of salt per 20 mL of sample.
Rinse equipment with salt solution to avoid dilution.
Filter the sample through funnels, allowing sediment to settle for 60 minutes.
4. Filtering:
Utilize distilled water, forceps, glass fiber filters, filter stand, aluminum tins, foil, and squirt bottles.
Rinse equipment with distilled water.
Quadrant the glass fiber filter.
Pour sample onto the filter, vacuum filtering, and dry for 24 hours.
5. Counting:
Use probes and a dissecting microscope for identification.
Differentiate plastics from sand, salt, and organic matter based on appearance and behavior under manipulation.
Notes:
Rinse equipment thoroughly between samples to prevent contamination.
Exercise caution and consistency during identification to ensure accurate results.
Do u publish this method? How can i put reference for this method?
I need the written version
which methods that suitable for analyzing microplastic?
Hello thank you for these informations I would like to have the written version if you may as you said.
Hi! Glad you're interested. If you'd like the written version please email me! You can find my email address under the "about" tab on my TH-cam page
What is the formula for 0.05 molar iron solution
Can anyone tell , what is concentration of salt solution used for rinsing
Is this process can be applied to Livestock Feeds?
Great video...may I know what mesh did you use?and may I know about written version?thanks
Thank you! If you send me an email, I can send you the protocol from this video. You can find my email under the "about" tab on my TH-cam page. In the meantime, the method in this video is similar to the method here marinedebris.noaa.gov/reports-and-technical-memos. The citation is Masura, J., et al. 2015. Laboratory methods for the analysis of microplastics in the marine environment: recommendations for quantifying synthetic particles in waters and sediments. NOAA Technical Memorandum NOS-OR&R-48
will you tell me about the how microplastic sampling will be done in the in effluent and and effluent of STPS and WWTPs. How the discharge of microplastics in MP/L or MP meter cubic per day will be determined. What are the appropriate sampling devices used for the microplastics in these units
You could do this by taking a grab sample of the water and measuring the volume of the water you have sampled or by leaving a net with a small pore size in the water for a measured amount of time and calculating the volume of water that traveled through your net. There may be other ways, but these are the ones I am most familiar with. Also, you would need to calculate the discharge of the river in order to calculate how many microplastics move through the river per day.
Hi Elizabeth,
thank you for the very nice and helpful video 🙂
May I ask you, is this protocol an adopted or similar version of the "NOAA Method
for the Analysis of Microplastics in the Marine Environment"?
Cheers, Simone
Yes! It's adapted from that protocol.
Did you actually publish the journal based on this protocol of yours . Can i see the journal or maybe you could give the doi instead.
This exact method is not published in a journal. However, it is adapted from a NOAA method that you can find here marinedebris.noaa.gov/reports-and-technical-memos. The citation is Masura, J., et al. 2015. Laboratory methods for the analysis of microplastics in the marine environment: recommendations for quantifying synthetic particles in waters and sediments.
How to i confirm there is a microplatics or plastic content??
Can I get the written study?
Whats the size of filter paper
Hy mam., I am planning to work on a microplastic project. I would like to know the specifications of the filters (microns, grade etc.) and the microscope (light/ compound/dissecting) used in the video?
We used a dissecting microscope and 0.7 µm glass-fiber filters from MilliporeSigma.
@@elizabethberg472 how to detect microplastic in freshwater
I wanna find the microbeads in cosmetics. And Analysis quantification of the range of microscope techniques SEM and TEM. How to do it? Can someone explain it.
I'm sorry. I have not used these methods, but there are studies that have. Perhaps you could start here doi.org/10.1016/j.marpolbul.2019.07.067
@@elizabethberg472 thank you so much
@@elizabethberg472 but can we use this method to extract microbeads from facial scrub or cleansers?
Can we use this method for extracting microplastics from personal care products?
I haven't, but I would imagine the method could be adapted for personal care products.
man bobs head and snaps fingers... "what did she say? I don't know music's too good... gotta feel that grove man..."
may I ask
what kind of microscope did you used? thanks
A dissecting microscope!
What is the reason behind of the adding iron, hydrogen peroxide, salt and others?
The hydrogen peroxide and Fe(II) solution are used to dissolve organic material. The salt is to separate the microplastics from the sediment, as the microplastic should float in the salt solution.
@@elizabethberg472 thank you so much
Hi, I would like to know about your research publications on microplastics... kindly share the details..
I am still in the process of writing up my papers, but if you look up the Hoellein and Kelly labs at Loyola University Chicago, you can see my work and the work of my collaborators!
Hi Elizabeth, is there any way to contact you
This protocol is based on what research journal?
This exact method is not published in a journal. However, it is adapted from a NOAA method that you can find here marinedebris.noaa.gov/reports-and-technical-memos. The citation is Masura, J., et al. 2015. Laboratory methods for the analysis of microplastics in the marine environment: recommendations for quantifying synthetic particles in waters and sediments. NOAA Technical Memorandum NOS-OR&R-48
Thank you for your nice vedio how can we connection with you please?
Hello, I am working on a project for school on the topic of microplastics. If you could help that would be great. Reply if you want
What is the concentration of your salt solution?
5.13 M NaCl, or 6.0 g per 20 mL water. This was the highest amount of salt we could dissolve.
Is this method suitable for seawater?
Yes!
I wanna do these experiment. Can you please help me?
which sort of help you want
Celsius... must be Canadian...
Could you please send me your Mail Id as I cannot find it under the about TAB on your TH-cam page.
I couldn'nt find your email address.
You can find my email under the "about" tab on my TH-cam page
Hlo maam.. ur email id is not available in your about tab.. kindly provide.. i need the written manual..
If you send me an email, I can send you the protocol from this video. You can find my email under the "about" tab on my TH-cam page. In the meantime, the method in this video is similar to the method here marinedebris.noaa.gov/reports-and-technical-memos. The citation is Masura, J., et al. 2015. Laboratory methods for the analysis of microplastics in the marine environment: recommendations for quantifying synthetic particles in waters and sediments. NOAA Technical Memorandum NOS-OR&R-48
@@elizabethberg472 Thank you maam..i have got the protocol from the given reference.thank you
Wow you did a very wonderful job. Please help and paste your email address for me to contact you. I need the written version of this work. I checked your about but I couldn't found your email address there.
Please help.
Warmest regards!
man bobs head and snaps fingers... "what did she say? I don't know music's too good... gotta feel that grove man..."
I need the written version
Hi! If you send me an email, I can send you the written protocol! You can find my email under the "about" tab on my TH-cam page!
It's under "for business inquiries" under the "about" tab on my page. You can get to my page if you click on my name!
Celsius... must be Canadian...