Radioimmunoassay technique (RIA)

This lecture explains about radioimmunoassay techniques or RIA. Radioimmunoassay (RIA) test is a very important in vitro assay method used to measure concentrations of antigens (for illustration, hormone levels in blood) through use of antibodies. As such, it may be visible because the inverse of a radiobinding assay, which quantifies an antibody via use of corresponding antigens.
Watch this video lecture to understand the mechanism of Radioimmunoassay also known as RIA.
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Thank you sir. I work in a Regulatory Science division. I needed to understand RIAs and now I do.

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Thank u sir,i really found that process hard to assimilate by just going through the books until I watched this video

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[@abdullahmarwat2714]

according to RIA when radiolabeled Ag combine with Ab ,the reaction will occur and it gives a signal like color graph or whatever so, but only free labeled Ag give no result. So concentration of Ag (in patient sample) is inversly propotional to the radioactivity.

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[@shrutihsingh]

That was great!! Thank u
But what we studied was there are blocking agent which gets displaced & also the supernatent is discarded rather than detected for radioactivity!! Also conc. is inversely proportional to radioactivity. I think i m more confused now!!

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awaesome way to express idea about the concept of ria and elisa no other video can substitute this video its a best video of ria

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[@alokdev6671]

It was a wonderful explanation thank you.My question is ,,the more radioactivity means the more antigen present in patient serum and it is directly proportional to the patient Ag serum but in case of hormone is it the same ...? because i read somewhere that hormone in the serum sample inhibits binding of the radiolabbelled hormone thus more radiactivity =less hormone .I would appreciate if you can reply me back to explain it furter .Once again thank you ...you are awesome

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Thank u for making this technique so easier......concept are well explained

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[@mimo5463]

Hi Shomu. Thanks for the lecture, very easy to understand and informative. I have a question however. I have watched several other videos on TH-cam that say when you have no unlabeled antigen bound to the antibodies, the radiation is at 100%. Here you are saying it's the other way around, that when the labeled antigens are bound to the antibodies it's at 0% (or close to it) and then when the unlabeled antigens compete and bind to the antibodies it is possible to measure radioactivity because of the "free" labeled antigens, meaning the % will increase...
Did I misinterpret something or are there just other ways to measure the radioactivity?

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How do they displace....if they already attached then how would newly added patient serum ag will compete...

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Sir please make video on immunological assay . I can understand your lecture very interestly .

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Okay

[@RitikaVishnoi9]

Sir i want to ask a question that the binding is competitive so why there is more affinity for non labelled antigen as the tagged antigen are removed after non labelled antigen is added. Can there be a case where the untagged antigens are unable to displace the tagged from the antibody.

[@60DeaRpooja]

Competitive binding is coz of conc. If untagged antigen conc. Is more then it ll displace more .otherwise there ll be less displacement.

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