Construction of maltose standard curve by DNS method - Amrita University

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Maltose is a disaccharide made up of two sub units of glucose monomers. It is also called malt sugar. It is present in germinating grain, in a small proportion in corn syrup, and is also a product of the partial hydrolysis of starch. Maltose is an important component in the process of creating fermented barley that in turn can be used to brew beer. Adding in a third unit of glucose produces a sugar known as maltotriose, while further units make it possible to produce maltodextrins.
Maltose reduces the alkaline solution of3.5 dinitro salicylic acid (DNS), which is pale yellow, into an orange-red complexof 3-amino --5-nitro salicylic acid. The optical density is measured at 540 nm. The intensity of the color depends on the concentration of maltose.

[@bedrosm]

Thank you so much, this is helpful for my son since he is doing science fair project that will need to develop Glucose standard using DNS.

[@ncs2000]

what does 2N NaOH mean? or does it mean 2M NaOH?

[@sahutchai]

Thank a ton