Per usual--great episode. I love listening to you discuss, as a group, topics like T/B cells, plasma cells, antibodies, viruses, RNA SEQ. So much passion. So fun. I love learning. Thank you!
Minor Correction: Single cell RNAseq shows you the transcripts that are present in individual cells, the downside is that doest not show you all the transcripts in a single cell, only the top bunch of genes. Also, it's the surviving subset of cells from the microfluidics required to separate these cells. It is a very powerful technology but it has these caveats.
Many thanks for this analysis. Am I right in saying that we still don't know if inflammation begins in the colon and the immune system response leads to a disease state or if it is a malfunctioning immune system that causes injury to the colon? I have a personal interest as I was diagnosed with UC in 2010 but after a short course of treatment and then a minor flare I have been in remission since 2013. My only medication is a low dose non steroidal anti inflammatory plus I follow a whole foods plant based diet. On diagnosis I was recruited into a research programme which means I follow any developments with interest. Thanks again and sending best wishes from the UK.
As a Crohn's patient of 40 years, I can tell you that when you discuss these related conditions, if your discussion doesn't revolve around the multiple types of dysfunction of the endocannabinoid system (ECS), you will miss the point. Western medicine is in the barbers and leeches stage when it comes to treating these conditions, however traditional Chinese medicine and Vedic medicine have addressed these conditions successfully for multiple thousands of years now through augmentation and/or stimulation of the ECS. I've heard it said the CB1 receptor is the most populous G-protein coupled receptor in human physiology, yet it was only identified in humans in 1992. Please reference arachidonoyl ethanolamide (anandamide) and 2-arachidonoyl glycerol (2AG) for more.
Hi Peter, I've had Crohn's for about the same time as you ... would you have any more information on the above? I'm not medically trained in anyway but I like to keep informed.
@@wattersdr Hi David, I've got a rather long file of bookmarks linking to scientific articles but for somebody just getting acquainted with this end of biochemistry, I would recommend general study of the human or rather the mamillion endocannabinoid system (ECS). It's absolutely fascinating and one of the largest and deepest rabbit holes of human biological investigation you'll find. The ECS is one of, if perhaps not one of the most important bioregulatory system in all vertebrates for the past 600 million years. We have a number of bioregulatory systems that are vital to continued life and health, but the ECS is unique in its role to maintain homeostasis between pro and anti-inflammatory immune response states, not to mention its role in the immune system's elimination of cancers through multiple parallel paths. In addition to a host of genetic neuroreceptor expression issues, dysfunction of the ECS can come in multiple forms ranging from underproduction of endocannabinoids (chiefly anandamide and 2-AG) to an overproduction of the enzyme FAAH (fatty acid amide hydrolase) which is responsible for enzymatic degradation of the endocannabinoids once they have performed their function. Endocannabinoids are transitory signaling lipids with are produced and consumed as necessary, in the ideal case. Chinese medicine was the first of the modern knowledge systems to document the role and maintenance of this bioregulatory system, and now, 5000+ years later, European descendant culture is just starting to look for understanding here. Sadly the science in this area has been long occluded by politics and sociological factors which become clearer once you connect in the only plant based alternative source of this chemistry. Oh my; I've opened the can of worms. Lol. I'll close this message with the acknowledgement that for my health, I moved to Colorado to get easy legal access to lab tested phytocannabinoid extracts which I use in the form of distillate extracts consumed via a vaporizer pen. Dose titration is as simple as metering the number of puffs per day and the feedback is so immediate that there's never a need to use more than necessary. The only downside is the transitory nature of the players in the ECS and thus its inability to be easily targeted by a 'time release' medication, so more active hands-on dose management is necessary but again it's a question of one puff or two in the morning (or 3-4 on a bad day). I do not take any Crohn's pharmaceuticals, and I'm only really feeling unwell in that respect for a few handfuls of days a year. I start every interaction with a new healthcare provider with questioning about their understanding of the ECS, and its role in maintaining health. The CB1 neuroreceptor may be the most populous G-protein coupled receptor in human physiology, but most physicians are without a clue of its existence or function. That last point leaves me flabbergasted considering the implications for health and disease management. Cheers and best wishes from Boulder.
@@JockNmystyle- Puff away at will without worry until you achieve what you want. I'm using a THC distillate pen right now to treat Crohn's Disease and I go through 250mg to 750mg daily these days to remain symptom free. That's about $4 - $12 a day here in Colorado. Dosage is a funny thing with cannabinoids because your system is constantly making them and enzymatically degrading them with fatty acid amide hydrolase (FAAH) so you don't really see dose-dependent reactions like you do with other compounds. The good news is you can't have too much and since the mental effects of CBD are almost zero you can consume away without any worry.
Brianne Barker, "Jeopardy!" Winner.
I missed it. How much did she win?
Per usual--great episode. I love listening to you discuss, as a group, topics like T/B cells, plasma cells, antibodies, viruses, RNA SEQ. So much passion. So fun. I love learning. Thank you!
Thank you for this discussion. Incredibly informative.
I think Brianne is trying to talk but nobody can hear her.
Minor Correction: Single cell RNAseq shows you the transcripts that are present in individual cells, the downside is that doest not show you all the transcripts in a single cell, only the top bunch of genes. Also, it's the surviving subset of cells from the microfluidics required to separate these cells. It is a very powerful technology but it has these caveats.
Many thanks for this analysis. Am I right in saying that we still don't know if inflammation begins in the colon and the immune system response leads to a disease state or if it is a malfunctioning immune system that causes injury to the colon? I have a personal interest as I was diagnosed with UC in 2010 but after a short course of treatment and then a minor flare I have been in remission since 2013. My only medication is a low dose non steroidal anti inflammatory plus I follow a whole foods plant based diet. On diagnosis I was recruited into a research programme which means I follow any developments with interest. Thanks again and sending best wishes from the UK.
As a Crohn's patient of 40 years, I can tell you that when you discuss these related conditions, if your discussion doesn't revolve around the multiple types of dysfunction of the endocannabinoid system (ECS), you will miss the point. Western medicine is in the barbers and leeches stage when it comes to treating these conditions, however traditional Chinese medicine and Vedic medicine have addressed these conditions successfully for multiple thousands of years now through augmentation and/or stimulation of the ECS. I've heard it said the CB1 receptor is the most populous G-protein coupled receptor in human physiology, yet it was only identified in humans in 1992. Please reference arachidonoyl ethanolamide (anandamide) and 2-arachidonoyl glycerol (2AG) for more.
Hi Peter, I've had Crohn's for about the same time as you ... would you have any more information on the above? I'm not medically trained in anyway but I like to keep informed.
@@wattersdr Hi David, I've got a rather long file of bookmarks linking to scientific articles but for somebody just getting acquainted with this end of biochemistry, I would recommend general study of the human or rather the mamillion endocannabinoid system (ECS). It's absolutely fascinating and one of the largest and deepest rabbit holes of human biological investigation you'll find. The ECS is one of, if perhaps not one of the most important bioregulatory system in all vertebrates for the past 600 million years. We have a number of bioregulatory systems that are vital to continued life and health, but the ECS is unique in its role to maintain homeostasis between pro and anti-inflammatory immune response states, not to mention its role in the immune system's elimination of cancers through multiple parallel paths.
In addition to a host of genetic neuroreceptor expression issues, dysfunction of the ECS can come in multiple forms ranging from underproduction of endocannabinoids (chiefly anandamide and 2-AG) to an overproduction of the enzyme FAAH (fatty acid amide hydrolase) which is responsible for enzymatic degradation of the endocannabinoids once they have performed their function. Endocannabinoids are transitory signaling lipids with are produced and consumed as necessary, in the ideal case. Chinese medicine was the first of the modern knowledge systems to document the role and maintenance of this bioregulatory system, and now, 5000+ years later, European descendant culture is just starting to look for understanding here. Sadly the science in this area has been long occluded by politics and sociological factors which become clearer once you connect in the only plant based alternative source of this chemistry. Oh my; I've opened the can of worms. Lol.
I'll close this message with the acknowledgement that for my health, I moved to Colorado to get easy legal access to lab tested phytocannabinoid extracts which I use in the form of distillate extracts consumed via a vaporizer pen. Dose titration is as simple as metering the number of puffs per day and the feedback is so immediate that there's never a need to use more than necessary. The only downside is the transitory nature of the players in the ECS and thus its inability to be easily targeted by a 'time release' medication, so more active hands-on dose management is necessary but again it's a question of one puff or two in the morning (or 3-4 on a bad day). I do not take any Crohn's pharmaceuticals, and I'm only really feeling unwell in that respect for a few handfuls of days a year. I start every interaction with a new healthcare provider with questioning about their understanding of the ECS, and its role in maintaining health. The CB1 neuroreceptor may be the most populous G-protein coupled receptor in human physiology, but most physicians are without a clue of its existence or function. That last point leaves me flabbergasted considering the implications for health and disease management.
Cheers and best wishes from Boulder.
@@Peter_S_ what dosage is good for a CBD vape pen?
@@JockNmystyle- Puff away at will without worry until you achieve what you want. I'm using a THC distillate pen right now to treat Crohn's Disease and I go through 250mg to 750mg daily these days to remain symptom free. That's about $4 - $12 a day here in Colorado. Dosage is a funny thing with cannabinoids because your system is constantly making them and enzymatically degrading them with fatty acid amide hydrolase (FAAH) so you don't really see dose-dependent reactions like you do with other compounds. The good news is you can't have too much and since the mental effects of CBD are almost zero you can consume away without any worry.
Dump ALL incumbents.