I have passed my biochemistry exams thanks to your videos. It would be fantastic to have you cloned and placed in to our lecture halls :) Thank you for all your effort!
AH man, I was so stressed about the lecture before I watch your video. what my prof. try to explain in 50 minutes you did just in 10 minutes. very very thankful.
"AK LECTURES" are wonderful videos. These lectures are continuously helping us to understand the most complex processes of Biology and Biochemistry. We really appreciate this great professor of Biology/Biochemistry. And we are really grateful to him.
Hat off to you sir. I bet many people will find in these videos the enthusiasm and skill to teach that many times is kacking in their classrooms. Brilliant performance, :)
thank you 😊 tomorrow is my test and I was running out of time to prepare it but you explained the topic so well that I am quite confident for tomorrow:)
Super,thanks! A stupid question but how does enhancer sequence just bind to the site as a promoter? Does the DNA just bind to itself? How does it get there?
+AK LECTURES I think you should distribute this video into two, one for prokaryotes while another for eukaryotes. Thank you very much for your lecture! I really appreciated it! #bigfanfromyou
3:30 There is a mistake in the video on the picture there is not a DNA template strand but CODING strand because mRNA is made in 5'-3' end direction and all the names on the DNA strand are given as are on codding strand of DNA not template.
On the whiteboard is a diagram of 5’ to 3’ DNA with the pribnow promoter box shown to the right of the 5’ end of a DNA single strand. Yet you say it is the compliment of this strand that is read by the RNA polymerase starting at the 3’ end of the not shown “template” strand. If so, then what is the nucleotide sequence for our pribnow promoter site? Shouldn’t the nucleotide sequence of pribnow be the compliment of the shown TATAAT? Which is really ATATTA? Can you see the dilemma here?
Great video! Thanks! Quick question. Is 35 and 25 distance from transcription in prokaryotes and 1000, 75 and 25 in eukaryotes hard distances, are they an exact average, or are they rough approximations?
They are only rough approximations because it might differs from different individual not every individual having the same amount of nucleotides in a certain strand of DNA hence when it was being transcript then the amount differs too. For approximations of -35 and -10 nucleotides upstream sequence are for prokaryotes while in eukaryotes are -25 to -35 nucleotides upstream sequence ( these are all just rough approximations ) nobody can give you the exact place for every individual as I mentioned it differs. :)
Really cool video! But I'd like to mention, that when you describe CAAT box for eucariotic cells, it is written on the board, that it is GGNCAATCT, which can not be true, because there is no nucliotide N. According to wikipedia, it starts as GGCCAATCT (N is changed to C).
Sir i follow Biochemistry Book by Donald Voet and Judith Voet . I found lot many details in this book do you have videous that talks about these details ... I found your videous very helpfull .. Thanks for nice lectures.. :)
I was watching your video on eukaryotic gene structure and there you said the RNA Pol II binds to the transcription start site. Now in this video you said it binds to the promoter. im confused....
Says DNA template, however isn't the DNA template strand the complement of RNA transcript? It should be the coding strand, because it is not the strand that is read.
+Jonathan Weiss i guess so yes.. and its promoter sequence ranges from -25 and -75. and its somewhat obvious if there wont be any transcription process then how ur body would be able to produce proteins, as we know that RNA helps in protein synthesis. I hope m clear to you.
hello sir, actually i didn't get what do u mean by bacterial gene>? i mean i know what is gene but how transcription can start from -10 units or -35 units from bacterial gene? i just didn't get that point. :D @aklectures thanks for delivering worthy lectures!
+Misbah Ashraf - I think the answer here is that RNA polymerase spans a large region of DNA before the gene. The core enzyme has a mass of hundreds of kDa... So the complex is large enough, and has enough active sites, to recognise promoter sites. Transcription does not start at these sites. Transcription starts at 0. -10 and -35 are in relation to transcription start.
+Misbah Ashraf The promoter region is just where RNA Polymerase will bind to the template. It does not actually start the transcription process until it gets to the start codon nearby it.
A genius is not someone who does very complicated things. A genius is someone who does very complicated things and make them look very simple.
I agree one hundred percent
when I go for lectures I just write down the topic and come and search for it from your videos. ..I mean u are the best teacher ever
I love how every single lecture he ends with the same face. I know he's just thinking "I slayed that damn lecture"
I have passed my biochemistry exams thanks to your videos. It would be fantastic to have you cloned and placed in to our lecture halls :) Thank you for all your effort!
+Arosya haha! thanks for the kind words, great job on your recent exam!
@@AKLECTURES you're the best, thank you so much for all your videos
AH man, I was so stressed about the lecture before I watch your video. what my prof. try to explain in 50 minutes you did just in 10 minutes. very very thankful.
"AK LECTURES" are wonderful videos. These lectures are continuously helping us to understand the most complex processes of Biology and Biochemistry. We really appreciate this great professor of Biology/Biochemistry. And we are really grateful to him.
Thank you so much, the notes I take during these videos are a thousand times more helpful than the ones I take in class
Your short videos are much more useful than the most of my university lectures!
I just find all your videos so useful and your explanations are so clear.Hat off to you, sir. Thank you for doing all of these videos
Hat off to you sir. I bet many people will find in these videos the enthusiasm and skill to teach that many times is kacking in their classrooms. Brilliant performance, :)
Red Orr thank you for the kind words! :)
thank you 😊 tomorrow is my test and I was running out of time to prepare it but you explained the topic so well that I am quite confident for tomorrow:)
I graduated from university 7 YEARS ago and you channel got me through ❤
Helped me while I was an undergrad and helps me now that I'm a grad looking back at the basics to understand more complex processes :')
splendid, even point out the minor details. i dont know how much grateful i m to your lectures. keeeeeeeeep up the good work. god bless you
You are very articulate...Thank you for creating videos which clarify complex ideas. You're amazing.
very well explained video, even for forein people! It will make me pass my biochemistry exam in one week :) Greetings from Germany!
From syrian Arab Republic thank you man 💙💙💙
You are a excellent teacher! Wish you could come and teach at my university. Thank you so much for your videos!
thank you so much for posting these videos. super helpful and you make subjects easier to tackle. Super appreciative!!
I love you, you make things look so much easier hahaha
You have a special gift.Thank you for sharing!
Dayna B Thank you for your kind words Danya!
Dicção perfeita!!!!!! Amei o vídeo e a explicação
It is written DNA template, isn't it nontemplate stand? Actually 3' to 5' one is template I guess.
you're a genius! Thank you!
I can't stop thanking you thank you doctor
Thank you, Sir, I am your biggest fan. Excellent work.
thank you for sharing, Really very helpful, cant thank you enough.... u r talented
Your my superhero in the medschool
Super,thanks! A stupid question but how does enhancer sequence just bind to the site as a promoter? Does the DNA just bind to itself? How does it get there?
how beautiful your explanation is !
excellent clarity! you are well understood
excellent explanation. you have made it simple to understand
I think I'll pay a lot for such teaching in my country, thank you
CAAT box sequence is wrongly mentioned as GGNCAATCT,rather than GGCCAATCT !
Why can't you be my professor! You explain everything so well, I actually understand it!
Do most eukaryotic transcriptions tend to separate via termination by a stem-loop signaling, or is more thanks to the function of RNA helicase?
Great lectures ever!
+AK LECTURES I think you should distribute this video into two, one for prokaryotes while another for eukaryotes. Thank you very much for your lecture! I really appreciated it! #bigfanfromyou
I thank you so much every single day!
3:30 There is a mistake in the video on the picture there is not a DNA template strand but CODING strand because mRNA is made in 5'-3' end direction and all the names on the DNA strand are given as are on codding strand of DNA not template.
It is written wrong but he is explaining it correctly
Sure
J
On the whiteboard is a diagram of 5’ to 3’ DNA with the pribnow promoter box shown to the right of the 5’ end of a DNA single strand. Yet you say it is the compliment of this strand that is read by the RNA polymerase starting at the 3’ end of the not shown “template” strand. If so, then what is the nucleotide sequence for our pribnow promoter site? Shouldn’t the nucleotide sequence of pribnow be the compliment of the shown TATAAT? Which is really ATATTA? Can you see the dilemma here?
your very helpful thanks your great keep it going
wooow !!
thanx for the amazing explanation
Thank you for the efforts
Pls sir, the strand the promoter regions are on, is it on the 5' to 3' or on the 3' to 5' . Thanks so much
Great video! Thanks! Quick question. Is 35 and 25 distance from transcription in prokaryotes and 1000, 75 and 25 in eukaryotes hard distances, are they an exact average, or are they rough approximations?
They are only rough approximations because it might differs from different individual not every individual having the same amount of nucleotides in a certain strand of DNA hence when it was being transcript then the amount differs too. For approximations of -35 and -10 nucleotides upstream sequence are for prokaryotes while in eukaryotes are -25 to -35 nucleotides upstream sequence ( these are all just rough approximations ) nobody can give you the exact place for every individual as I mentioned it differs. :)
Awesome expalanatin..thank you sir..
neha verma you're welcome :)
Really cool video! But I'd like to mention, that when you describe CAAT box for eucariotic cells, it is written on the board, that it is GGNCAATCT, which can not be true, because there is no nucliotide N. According to wikipedia, it starts as GGCCAATCT (N is changed to C).
N stands for anything
Sir i follow Biochemistry Book by Donald Voet and Judith Voet . I found lot many details in this book do you have videous that talks about these details ... I found your videous very helpfull .. Thanks for nice lectures.. :)
Very useful, thanks a lot🙏
I was watching your video on eukaryotic gene structure and there you said the RNA Pol II binds to the transcription start site. Now in this video you said it binds to the promoter. im confused....
Question
Tata box sequence is located on template strand or coding strand ???
Great Video, keep it up
this video is not inside one of your playlists, is it? If it is, I want to see that playlist!
Says DNA template, however isn't the DNA template strand the complement of RNA transcript? It should be the coding strand, because it is not the strand that is read.
sir you are the best
Thank you very much doctor
Awesome lecture 👌
The BEST TEACHER
Hi thank you for all your videos. Do you have more please especially on biochemistry
check out the website in the description.
Amazing lectures
감사합니다
do you have an RNA transcription for eukaryotes??
+Jonathan Weiss i guess so yes.. and its promoter sequence ranges from -25 and -75.
and its somewhat obvious if there wont be any transcription process then how ur body would be able to produce proteins, as we know that RNA helps in protein synthesis.
I hope m clear to you.
You are a saint
Legend 😍😍😍😇😇😇thanks a ton
which lecture involves the rho?
am so confused where is the promoter located is it on the sense dna or what plz answer me
Can i just know ,what does N stands in CAAT box please.
You are my hero.
*looks at top right corner of whiteboard* - Are all eukaryotic genes called Eugene? hehe. Thank you once again!
You are Fantastic
thank you soo much!!
He literally prints a whole guide on his white board.
hello sir,
actually i didn't get what do u mean by bacterial gene>? i mean i know what is gene but how transcription can start from -10 units or -35 units from bacterial gene? i just didn't get that point.
:D
@aklectures
thanks for delivering worthy lectures!
+Misbah Ashraf - I think the answer here is that RNA polymerase spans a large region of DNA before the gene. The core enzyme has a mass of hundreds of kDa... So the complex is large enough, and has enough active sites, to recognise promoter sites. Transcription does not start at these sites. Transcription starts at 0. -10 and -35 are in relation to transcription start.
+Misbah Ashraf
The promoter region is just where RNA Polymerase will bind to the template. It does not actually start the transcription process until it gets to the start codon nearby it.
Genius!
why transcription factors are in eukaryotic transcription but not in prokaryotes?
Woooooooooow awesome 👌 💕 👌 well explained, thanks so much
Sir can you please give lecture about translation
Why do has the mRNA of prokaryotes no promoter?
pefect.....thank you :)
Mohamed Emad You're welcome!
Well that’s it, this is how I pass Cell Biology.
You are incredibly a nice teacher, kindly improve on your conclusive remarks.
bless you
What does the N denote in the CAAT box
+Cognitive Philosophy Don't quote me on this, but I think it just means nucleotide, as in any base can go there.
+holohedx2 yes it does
why can't you be my biochem professor :(
LOL we look alike, a little bit. only 99.99999999% identical.
boss