Does media and transfer/passage number affect colony morphology? Different media? Should I care? No?
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It's obvious! The mycelium's favorite colour is green.
It seems the concensus is that if your plate is nutritional then you get tomentos growth. If your plate has just enough nutrients then you get rizmorphic growth. Reason being is because it is stretching out to find food versus concentrating in a smaller spot.
Exactly. I stopped caring so much though because my main point is to make spawn and fruit. Growth on agar doesn't seem to affect anything else.
Thanks for answering my question! Super helpful information
@Edward Grand I'm continuing the motivation i got from your live stream today and I have 10 pre poured Petri dishes that been in the refrigerator for about a month (which is longer than I was planning).
I plan on taking them out and put them with the agar at top and let them come to room temp.
I imagine they're going to condensate with the temperature change..
Is there any other tips or advice you can give me for doing this for the very first time?
I hope you have an awesome weekend Ed‼️
Love to hear that people try only for rhizo when all mycelium is good if it’s not contaminated
I'm always trying to keep my agar exactly the same. Trying to eliminate as many variables as possible. It's amusing to hear you say you are fairly haphazard with yours.
ed one of the best out here man !!
After you confirmed the 5th and 6th plate were clean, why don't you recommend also using the 4th, 3rd, and 2nd plate too?
What do you mean with sub and mono culture? I dont understand that part :/. Can you pls explain me that :)) Best greetings
thanks
Morning!!🎉🎉
Hey Ed I noc up a couple bags with LC tonight and I had 11cc in syringe! I wanted to put 5 & 5 in each bag an one CC in an agar slant. But I accidentally put 6 cc in last bag and was only able to basically put what ever was in the syringe which basically looked like water. Do you think I’ll get any growth in that slant?
Yes
Ty Ed appreciate you can’t wait till I get my agar plates. your knowledge has helped me understand so much.
@@edwardgrandDifferent media hard influences colony? For example mae vs dog food
@@MonsterMushhead bro. Don’t buy plates. It’s literally as easy as boiling water.
@@PPP-on3vlThe media recipe definitely has an effect on colony growth and morphology. It is good to change the recipe once in a while, but my go-to is this:
GRYA recipe
2% Glutinous Rice/Whole wheat flour
2% Agar
0.1% Baker's or Nutritional Yeast
Food Coloring (if desired)
FOR 500 ml Total volume
10 g Glutinous Rice/Whole wheat flour
10 g Agar
0.5 g Baker's Yeast
Food Coloring or 1g charcoal if desired.
500 ml tap water
- Should make between 20-25 standard size plates.
Nice “teaching moment”*
I’m also inclined to be intentionally casual with agar recipes/ formulations, also throwing in some rando stuff now and then. My question for you is, if you are doing that and then find a medium that interacts well with a particular culture / cultures in general… it would also be great to repeat it… So I’m asking when you are making up varieties of agar, do you keep a recipe for all your Tweeqs - so you can repeat it if you find a winner? *(unplanned stuff that is instructional if you keep an open mind and apply some critical thinking to understanding the surprise)
🎉🎉 good morning
Hi Ed, is it normal for cultures on agar dishes to smell and have a tan color after a month of starting
Strange smell is almost always a no go
Thanks, and is the tan normal?
@@Kilwa1976 depending on the genetics themselves and the agar dish ingredients, they can end up eating the food coloring resulting in a tan or white plate. Usually that’s a sign that it’s time to transfer again.
Nice Ed , thanks for the info
You're welcome. Thanks for watching
Love this style of video! I get alot of straight forward useful knowledge from you!
Me too, It’s refreshing to listen to someone who knows more than most but doesn’t pretend to be an expert
Might test the pH of the media in each of them. Who knows though.
For sure. The pH and recipe will definitely change morphology. It seems weird, but I am getting to the point where I purposely don't care about the look of the plate. It still bothers me to see an ugly plate, but I suppress my OCD because I know if it's clean, it'll be fine when it gets back on some 'normal' media. It's hard though. :)
Hey! Awesome vid! I got into agar last year and was very excited because I got germination and within 1 transfer had like 3 or 4 clean plates so I went to grain and then monotub. I tried 2 different monotubs and they colonized great and then just sat there for more than a month and never ended up fruiting. I ended up giving up and have not tried since a couple of weeks ago. I got all my stuff out again and poured some DFA plates and decided to go back to spore and try again. I guess my question is, if you know why certain colonies don't seem to fruit? It was from MSS, the mycelium was very tormentose, im wondering if it was my fault for not transferring from the correct areas? Thanks.
Copy/paste from an earlier comment:
GRYA recipe
2% Glutinous Rice/Whole wheat flour
2% Agar
0.1% Baker's or Nutritional Yeast
Food Coloring (if desired)
FOR 500 ml Total volume
10 g Glutinous Rice/Whole wheat flour
10 g Agar
0.5 g Baker's Yeast
Food Coloring or 1g charcoal if desired.
500 ml tap water
- Should make between 20-25 standard size plates.