How to Equilibrate HPLC Column?
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- เผยแพร่เมื่อ 3 ต.ค. 2024
- #hplc #interview #pharma #chemistry
How to Equilibrate HPLC Column?
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Sir please make video on selection of buffer in HPLC
There is one in Channel
Good sir
Sir could you explain why shipping solvent necessary for silica generation.
Thank you sir 🙏🙏
Sir I want skip testing
How to treat sample for acidified force degradation.
You reply force degradation with 1N HCl at 60°c if degradation is less than 5% then go with higher normality
I want to know should I use 0.1 nHCl and 0.1 nNaOH and 3percny H202 for oxidation for sample preparation of impurities generation in force degradation.Lastly how to perform neutralization bfore injecting in hplc
@@shivamtyagi4765 dear I also asked how do I use 0.1 n HCl and NaOH for sample preparation?
I mean if I have diluent in my method is buffer and methanol. So should I replace altogether diluent with 0.1 NHCl and NaOH?
No you don't remove diluent firstly you add 10 ml 0.1 HCl for degradation after that add 10 ml naoh 0.1 then make up with your diluent
You mean 10 ml as defined standard amount for every product for degradation purpose? Or amount of HCl can be change?