Theory of Column Selection in HPLC Method Development

Column selection based on Molecular structure and Stationary Phase
London Dispersion Forces
Dipole-Dipole Interaction
Hydrogen Bonding
Polarity vs Functional group
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[@rahulkulkarni3037]

Perfect explanation, will help us to choose right column while method development 👏

[@saikiran-nq2sn]

Good explanation about compound interactions with the columns. Thank you

[@sabarikrish9405]

Your videos are damn handy. Great work sir.

[@vivekchauhan4716]

Very nice explanation with good examples 👍

[@vaithylingam6119]

Useful information, please keep going on ......Thank you

[@bobbalisandhya4861]

Thank you very much sir for your outstanding explanation

[@mkche2036]

Very nice and make one video with hillic column with example

[@Ben_Kaushalesh]

Excellent explanation.

[@hemantsharma7128]

Very informative presentation

[@bharathigangireddy3467]

Why using octylamine as HPLC mobile phase in sodium nitrate content

[@shivareddyg9144]

Good Explanation sir.

[@gray12566]

Do you have a video on the peak area formula?

[@AnnadathaSrinu]

This presentation is very useful, but I have one question in example-1 , even prednisone also having OH group, it can also form hydrogen bond with amide column, but u Said prednisolone will form hydrogen bonding with amide column, can you please give me clarity

[@anushan9580]

Thank you so much sir

[@DipakBhaste-dn2kj]

How to seperate isosorbide 2-mononitrate and isosorbide 5-mononitrate....isomerse

[@bildadonyango354]

Thanks you so much

[@jitendratarmale6310]

Make video on HPLC calibration 🔥🔥

[@joywithsnehal]

Thanks 🙏

[@vadlavallikanakarao3154]

Good information sir

[@apnacoffeebreak7427]

Very Nice Presentation sir,💐
What is the main role of Endcaping reagent in column?

[@pharmagrowthhub3083]

To avoid secondary interaction of analyte with ionized silanol groups

[@buddepumohanarao8309]

Thanks sir and👌👌

[@jaykumarbhatt8841]

Very Very nice 👌 sir.

[@abhishekjoshi9650]

I am daily watching video

[@junimyerainfo6101]

I have a doubt sir what is landan dispersion

[@vishnupatil1345]

Hello sir. My sample is HCl salt of Amine, while doing HPLC developement it is coming in void. Can you help for this issue. How can I retain longer my sample peak.

[@pharmagrowthhub3083]

It looks like your compound is polar. Go for polar columns like amino or diol. If this doesn't work- ion pairing can be a last resort...

[@talluriramesh372]

Put more examples a bout this topic sir pls

[@vikaspundeer7242]

What's effect of RT when mobile phase highly polar and why

[@Pharmauncle]

Early elution bcoz highly polar compound first

[@vadlavallikanakarao3154]

We need system startup procedure slide also sir

[@krishnendukundu6446]

Sir plz refer some good book

[@gopalgirase-01]

Sir,If possible speak in Hindi.

[@milandoshi7640]

be careful, there are so many mistakes in this video

[@mkche2036]

What are the mistakes