Hello! Thank you for your video this topic is definitely cause for confusion. I am stuck on chapter 8 and hoping you could clarify where my thinking error is? I have an RNASeq exp with 3 time points and 3 treatment types+a control(DMSO). I run DESeq() as below twice, once using the 24hour as a reference point and once for the 48hour. I believe I should be extracting the same result below as you explain in your video (here treatment effect "Ibr" at 48 hours), but the results differ. design
hi, very well explained, thanks. question: I have a set of data which has this condition: 1. severe drought in short time 2. severe drought in long time 3. mild drought in short time 4. mild drought in long time now, I want to make a meta data but I'm not sure whether I should make one single column which contains all the conditions and their controls or I should make separate columns for short + long + severe + mild any suggestion would be appreciated.
Is day 7 where dispersion and other estimates explained uploaded anywhere? I would love to see know what is explained there.
Hello! Thank you for your video this topic is definitely cause for confusion. I am stuck on chapter 8 and hoping you could clarify where my thinking error is?
I have an RNASeq exp with 3 time points and 3 treatment types+a control(DMSO).
I run DESeq() as below twice, once using the 24hour as a reference point and once for the 48hour.
I believe I should be extracting the same result below as you explain in your video (here treatment effect "Ibr" at 48 hours), but the results differ.
design
hi, very well explained, thanks.
question:
I have a set of data which has this condition:
1. severe drought in short time
2. severe drought in long time
3. mild drought in short time
4. mild drought in long time
now, I want to make a meta data but I'm not sure whether I should make one single column which contains all the conditions and their controls
or I should make separate columns for short + long + severe + mild
any suggestion would be appreciated.
Hey! I also have similar case. Did you find a way to design your conditions? Thanks in advance!