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Single Beam- Apparatus:- A spectrophotometer measures the light that passes through the sample, to then strike the detector, where it is measured. Let’s call the amount of light at wavelength λ incident on the sample cuvette Io and the amount of light exiting the sample to hit the detector I. If we now calculate the percent of the ratio these two values (I/Io)*100 we get a percent transmission (%T) value. If the sample transmits all the light at a given wavelength then %T = 100; however, if the sample absorbs light then we will have the case where I < Io and %T will be a number less than 100. If the beam is totally blocked or absorbed by the sample, then %T = 0. One more important item. When a spectrophotometer is turned on it has not been calibrated for the values of 100 %T or 0 %T. This 100 %T calibration is acquired by a process called background correction for a spectrum. it is also sometimes called auto-zero for a single wavelength measurement. A background correction is performed by removing the sample from the instrument and measuring the amount of light that strikes the detector in the scan range of all wavelengths in the spectrum. These 100 %T values (called a background scan) are then stored in memory for use in calculating accurate sample %T values for a sample.
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@@atqasiddiqui3418 ya mutlb ha Respected Students 🙏🙏🙏 Aik video lecture ko final krr k upload krny mein 5 to 6 hours lagty hn Pahly lecture notes ready kro Pher lecture easy krr k samjoy Recording kro Editing kro software sa Rendering kro software sa Uploading kro Pher students share tk Nahii krty Channel subscribers tk nahii krty 81 % viewers subscribe ka button bhe nahii press krty, Aksar students video lectures download krr k hamra nuqsan krr daty hn Mujy aik Pisa bhe Nahii milta Kharcha har har cheez kaa alag sa jaib sa hota Guzara Nahii hota beta Isy liya Photon Online Science Academy shroo k ha Jiss naa jo parhna haa Welcome Ay munaisb fees daa Parh lay Free parha parha krr khatum ho gia hn Jo jo topics jis students na parhny hn topic kaa naam btya, Class conduct karin gy notes provides karin gy recordings send karin gy inshallah topic cover ho Jay gaa Regards
Sorry to hear that.. I am not a chemistry student.. M doing pharm D just needed that for an assignment. May Allah bless you with more. Well i have subscribed when i really liked the lecture. May you succeed.. Amen
, what is a blank solution in spectrophotometry? The 'blank' solution will contain everything that the 'unknown' solution (the one you want to measure) except for the think you wish to measure.
Blank solvent is liya use krty hn k jiss solutions k hum spectroscopy krnaa chaty hn os mein jo solvent haa oss k solvent k effect koo zero krny k liyaa czz humm solute k bary mein information lenaa chaty hn so solvent k effect koo khtm krny k liya blank solvent use krty
@@chemistrybysaadanwar thanks for you respectable replied me from Kashmir India your voice is heart touchable, the ist time I have heard our pure Urdu language
Thank you so much sir!!! Very well explained Masha Allah! You literally saved me few hours before exam..
It Was very difficult and long in books but you make it easy respected Sir g 🌟 🌹 🌹 🌹
Can spectrophotometer can be used as fluorometer?
Don't know
Buhat badiya Allah ap ko khush rakhe
Thank you so much I m really glad to see your comments
@@chemistrybysaadanwar same here dear sir, according to our prophet" To respect your teachers your knowledge ability should be improved"
❤ well explaination Sir G✨
Very help full for everyone thnxu sir for making these video
Momina thank you so much for your feedback comments
&encouraging words
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@@chemistrybysaadanwar yes sir
Thank you so much
@@chemistrybysaadanwar don't say thank you
You deserve that much .
Single Beam- Apparatus:-
A spectrophotometer measures the light that passes through the sample, to then strike the detector, where it is measured. Let’s call the amount of light at wavelength λ incident on the sample cuvette Io and the amount of light exiting the sample to hit the detector I. If we now calculate the percent of the ratio these two values (I/Io)*100 we get a percent transmission (%T) value. If the sample transmits all the light at a given wavelength then %T = 100; however, if the sample absorbs light then we will have the case where I < Io and %T will be a number less than 100. If the beam is totally blocked or absorbed by the sample, then %T = 0.
One more important item. When a spectrophotometer is turned on it has not been calibrated for the values of 100 %T or 0 %T. This 100 %T calibration is acquired by a process called background correction for a spectrum. it is also sometimes called auto-zero for a single wavelength measurement. A background correction is performed by removing the sample from the instrument and measuring the amount of light that strikes the detector in the scan range of all wavelengths in the spectrum. These 100 %T values (called a background scan) are then stored in memory for use in calculating accurate sample %T values for a sample.
Thank you sir your video is very helpfull for me.
You're welcome 🙏💐
Very Nice Explanation sir👌👌
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@@chemistrybysaadanwar Sure Sir👍👍
Sir doble beam spectrometer k notes to mil gy hn single k nhi mile hn uska link share kar den plz
🙏🙏🙏🙏
Graph sy calculations krna b btaden unknown conc. Ki..
🙏🙏
Kya mtlb is emoji k
@@atqasiddiqui3418 ya mutlb ha
Respected Students 🙏🙏🙏
Aik video lecture ko final krr k upload krny mein 5 to 6 hours lagty hn
Pahly lecture notes ready kro
Pher lecture easy krr k samjoy
Recording kro
Editing kro software sa
Rendering kro software sa
Uploading kro
Pher students share tk Nahii krty
Channel subscribers tk nahii krty
81 % viewers subscribe ka button bhe nahii press krty,
Aksar students video lectures download krr k hamra nuqsan krr daty hn
Mujy aik Pisa bhe Nahii milta
Kharcha har har cheez kaa alag sa jaib sa hota
Guzara Nahii hota beta
Isy liya Photon Online Science Academy shroo k ha
Jiss naa jo parhna haa
Welcome
Ay munaisb fees daa
Parh lay
Free parha parha krr khatum ho gia hn
Jo jo topics jis students na parhny hn topic kaa naam btya, Class conduct karin gy notes provides karin gy recordings send karin gy inshallah topic cover ho Jay gaa
Regards
Sorry to hear that.. I am not a chemistry student.. M doing pharm D just needed that for an assignment. May Allah bless you with more. Well i have subscribed when i really liked the lecture. May you succeed.. Amen
I may join that. In my mphil . i.A when i need more practical work
Good explanation. .
Love from Indian occupied kashmir. .
Thank you so much Dear Aadil, I m really glad to know this
Stay blessed
@@chemistrybysaadanwar
I am also very glad to interact with you. .
Aadil thank you 💐🥰
India occupied kashmir😂😂😂😂. Bc Pakistan b pele India m tha sale mulle
Sir link par click kiya
Lekin pata nai chal raha notes kesy nikalny ha
?
Great explanation sir love from India
Explained very well.....
Thank you so much
Great Saad..keep it up!
Bhoot shukriya bhhot shukriya Umer bhii
Thanks for Appreciation
Stay blessed
Yesterday I was thinking about your videos as well ❤️💖
Saad bhai thanks but they are not good enough..i need consistency and improvement
@@Quaidian every one need it Yr,
I think just consistency needed yra
Method of teaching 👍👍👍👌👌👌
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Please support us by sharing the link of these educational videos with your class fellows and other students 🙏
Great video and easy methods ♥️
Thank you so much
Stay blessed
Lajawab lecture sir g
Thank you so much jee
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Sir single beam or double spectrometer ke use kis liye hota he
Spectroscopy techniques hn, uses or applications of spectroscopy or spectrophotometer k naam saa search krrnaa please
concentraion pta chlti h unknown sample ki ..phle spectrum graph bnega fr values ko compare krke hum unknown sample ki value bta skte h
Exactly
জাজাকাল্লাহ খাইরান!
Good explanation
Shukriya
Nice explanation sir thank u
Taimoor thank you so much for your comment
@@chemistrybysaadanwar pleasure Sir
Taimoor can you please support us by sharing the link of these educational videos with your class fellows and other students 💐🙏
@@chemistrybysaadanwar sure sir
Thank you
Numerical Pratice Vdo upload pls
Playlist mein kuch numericals toa hn
Sir iski theory plz
Sir agr blank soln mn se 100% transmitance hone hote ha to wo to us k bgr b ho skte ha....to hum blank soln q use krte hn???
, what is a blank solution in spectrophotometry? The 'blank' solution will contain everything that the 'unknown' solution (the one you want to measure) except for the think you wish to measure.
Blank solvent is liya use krty hn k jiss solutions k hum spectroscopy krnaa chaty hn os mein jo solvent haa oss k solvent k effect koo zero krny k liyaa czz humm solute k bary mein information lenaa chaty hn so solvent k effect koo khtm krny k liya blank solvent use krty
@@chemistrybysaadanwar ok sir
...thnk u so much...
Your welcome
👍👍
Sir g u from?
Saad
Pakistan
Well explained
Thank you
Great sir
arsalan ty
Nice
Thank you
Jai Shree Ram...🚩❤️ Good Explained ...👍
🌹💐❤️👍
9:23
👍
Wsalam
Great
Ustaad ap kaha sa ho aur apna contact number share keejia
Please see about section of this channel we shared all the information there
@@chemistrybysaadanwar thanks for you respectable replied me from Kashmir India your voice is heart touchable, the ist time I have heard our pure Urdu language
Great Sir
Good
Thank you