Agar has contains some level of sugar in it which would be a breeding ground for mold or fungus to grow. Especially since these plantlets will be in a moist/humid environment as they try to acclimate in the trays
Guessing game on the hormones remaining too. Auxins and cytokines used in plant tissue culture and not well studied for biological hazards with animal tissue. Indole acetic acid (IAA) most common in this stage of the process.
I’ve been told by my seller I should apply some fungicide in the deflasking process. Do you think it’s necessary? I have some tc’s that I plan to put in a nursery box so try moss
Usually online vendors will sell Tissue Culture (TC) plants. A google search will give you some good results . Jacks Bean Stalks in Florida is where I have purchased my individual Tissue Culture plants
It’s easier to separate the tissue cultures with tweezers I find. Especially if the roots are tangled. It helps to reduce the risk of introducing bacteria to the TC so long as the tweezers are sterile. I also, just don’t like touching jelly materials.
@@leafletco thank you! I had a lot of root rot when I started to be a plant addict but then someone told me to water by the bottom and it's really efficient
why do you need to remove the agar? it's not harmful, is it?
Agar has contains some level of sugar in it which would be a breeding ground for mold or fungus to grow. Especially since these plantlets will be in a moist/humid environment as they try to acclimate in the trays
Guessing game on the hormones remaining too. Auxins and cytokines used in plant tissue culture and not well studied for biological hazards with animal tissue. Indole acetic acid (IAA) most common in this stage of the process.
I’ve been told by my seller I should apply some fungicide in the deflasking process. Do you think it’s necessary? I have some tc’s that I plan to put in a nursery box so try moss
Thank u for your video. What are those black things in your soil mix?
So cool!
Where did you purchase these little babies? Local plant shop or online?
Usually online vendors will sell Tissue Culture (TC) plants. A google search will give you some good results . Jacks Bean Stalks in Florida is where I have purchased my individual Tissue Culture plants
Thanks for the info. 💚 looks so easy.
Please post an update
My notifications are all the way on
Thank you
Did you water your medium before hand?
no I dampened the soil just a touch and would monitor them to make sure they dont get root rot
@@leafletco if they did what were you planning on doing ?
Is there a special reason you use twizzers?
It’s easier to separate the tissue cultures with tweezers I find. Especially if the roots are tangled. It helps to reduce the risk of introducing bacteria to the TC so long as the tweezers are sterile. I also, just don’t like touching jelly materials.
how much do you water the plantlet?
To be honest, I just moisten the soil as I am scared of root rot.
@@leafletco thank you! I had a lot of root rot when I started to be a plant addict but then someone told me to water by the bottom and it's really efficient
But what's the differences?
Saleudo